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    Selenium biofortification of three wild species, rumex acetosa l., plantago coronopus l., and portulaca oleracea l., grown as microgreens

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    Microgreens of wild herbs are a source of healthy compounds. Selenium (Se) biofortification of microgreens could help increase the Se content and thus contribute to Se requirements in humans. We evaluated whether three wild herbs, Rumex acetosa L., Plantago coronopus L., and Portulaca oleracea L., were suitable for biofortification in order to obtain products with high nutraceutical value. In the first experiment, the three species were enriched with Na2 SeO4 at 0 and 1.5 mg Se L−1, and the effects of Se on the nutraceutical characteristics of microgreens were evaluated. In the second experiment, using P. oleracea enriched with 0, 1.5, 5, and 10 mg Se L−1, we investigated whether there was a relation between the increasing Se concentrations in the nutrient solution and the Se content in microgreens. The Se added was taken up by roots and accumulated in the aerial part. P. coronopus exhibited the highest ability to accumulate selenium, and the Se-enriched microgreens showed the highest chlorophyll and flavonoid content. The strong correlation between the Se concentration in the growth solution and the Se accumulated in P. oleracea may enable the cultivation of microgreens with the targeted Se content. The resulting Se-biofortified microgreens of wild species could represent a new vegetable product with high nutraceutical value also ensuring a sufficient dietary intake of Se

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Biofortification of Lettuce and Basil Seedlings to Produce Selenium Enriched Leafy Vegetables

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    Selenium (Se) biofortification of plants has been recognized as a good strategy to improve the nutritive value of vegetables and increase Se daily intake in humans. Identifying the most appropriate method to enrich plants is a key issue in the biofortification process. We tested a biofortification technique that produces Se enriched seedlings for transplant, yet barely modifies conventional cultivation techniques. Lettuce (Lactuca sativa L.) and sweet basil (Ocimum basilicum L.) were exposed to selenium by adding 0, 1 and 3 mg L−1 (lettuce) and 0, 2 and 3 mg L−1 (basil) of Se, as sodium selenate, to the growing substrate immediately after sowing. When seedlings reached an appropriate size, they were transplanted into the open field, and plants were grown until maturity. Lettuce and basil seedlings accumulated selenium without any reduction in leaf biomass at maturity. The highest dose of Se induced a higher antioxidant capacity and flavonoid content in both species at both sampling times. At maturity, biofortified plants still showed a higher leaf Se content compared to the control, and would be able to provide from 10% to 17% (lettuce) and from 9% to 12% (basil) of the adequate intake (AI) of Se

    Iodine biofortification of sweet basil and lettuce grown in two hydroponic systems

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    Two hydroponic techniques (floating system and aeroponics) were assessed for iodine (I) biofortification of sweet basil (Ocimum basilicum L.) and baby-leaf lettuce (Lactuca sativa L.). Iodine was supplemented by adding KI into the nutrient solution to achieve a final I concentration of 10 μM. Shoot biomass production and leaf concentration of I, nitrates, total phenols and pigments were measured on the occasion of two successive cuts, 14 and 28 days after transplanting. In both the hydroponic systems, the supplementation of KI represented an effective method for the biofortification of basil as it did not affect the plant growth, while it moderately reduced the biomass production in lettuce. Leaf I accumulation occurred to a greater extent in aeroponics than the floating system in both species. In KI-treated basil plants, leaf I content ranged between 9.76 and 23.58 mg kg− 1 FW. Consequently, 6 g of fresh basil leaves, which is contained in a portion of Italian pesto sauce (12 g), could satisfy 40%–94% of the Recommended Daily Intake (RDI) of iodine for healthy adults (150 μg day− 1). In lettuce, leaf I contents of first-cut leaves were 1.55 and 3.60 mg kg− 1 FW, in the floating system and aeroponics, respectively. Therefore, a serving size of 26 g of lettuce containing I from 1.55 (floating culture) to 3.60 (aeroponics) mg kg− 1 FW could satisfy 27 % to 62 % of the RDI of iodine. Lettuce grew much less in the floating system than aeroponics, probably because of the lower dissolved oxygen level in the nutrient solution as compared to aeroponics. Basil was less sensitive to the oxygen availability in the root zone, since no differences were detected between the two hydroponic systems in terms of fresh and dry biomass

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    Dispelling the Myths Behind First-author Citation Counts

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    We conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more sophisticated methods

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    Biofortification of Ocimum basilicum L. Plants with selenium

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    Sweet basil (Ocimum basilicum L.) is an aromatic annual plant belonging to the Lamiaceae family, cultivated and utilized throughout the world. Basil has a culinary value, and pharmaceutical properties. The aromatic oil extracted from its leaves has antioxidant and antimicrobial activity. Selenium (Se) is an essential element for humans, and acts as antioxidant and anticarcinogenic agent. Since healthy nutrient plant compounds are more bioavailable than compounds contained in food supplements, biofortification of fruiting and leafy vegetables with Se is a good way to increase consumption and supplementation of Se by humans. Further, Se appears to be effective in delaying fruit ripening and plant senescence, thus decreasing postharvest losses due to enhanced antioxidation. We investigated the effect of Se addition on production and quality traits of Ocimum basilicum L. 'Tigullio'. Two experiments were conducted on sweet basil seedlings hydroponically grown under greenhouse conditions at rate of 0 (control), 0.5, 1, 2, 4 mg Se L-1. Selenium was added to the nutrient solution as sodium selenate. In order to evaluate the growth, physiology, and nutritional value of basil plants the following parameters were determined at harvest time: plant biomass, antioxidant capacity, total phenols, rosmarinic acid, nitrate, macro- and micronutrients, total chlorophyll and total carotenoid. Our results showed that the addition of selenium significantly and linearly increased the Se content in basil. In general, selenium did not affect the plant biomass and the quality of product. The amount of Se accumulated in plants could provide the rational Se intake for human nutrition in accordance with the recommended dietary allowance (RDA) guidelines
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