3,217 research outputs found
VECTOR-BORNE DISEASES IN COLONY STRAY CATS OF MILAN CITY
LEISHMANIA INFANTUM INFECTION IN STRAY CATS IN A NON-ENDEMIC AREA IN NORTHERN ITALY
E. Spada, DVM, PhD, Researcher 1, A. Della Pepa, DVM 1,
A. Migliazzo, DVM, PhD 2, G. Bagnagatti De Giorgi, DVM 1,
R. Perego, DVM, PhD 1, D. Proverbio, DVM, PhD, Professor 1
1Dipartimento di Scienze Veterinarie per la Salute, la Produzione Animale e
la Sicurezza Alimentare, Universita degli Studi di Milano, Milan, Italy
2Centro di Referenza Nazionale per le Leishmaniosi,Istituto Zooprofilattico
Sperimentale della Sicilia, Palermo, Italy
Tipologia: Ricerca Originale
Area di interesse: Medicina interna
Purpose of the work.
To assess the prevalence of leishmaniosis in a large representative sample of stray cats from this non-endemic area, and to analyze the results according to clinical, laboratory and infectious data
2. MOLECULAR STUDY ON VECTOR-BORNE INFECTIONS IN URBAN STRAY COLONY CAT IN NORTHENRN ITALY
Eva Spada§, DVM, PhD, Researcher
Daniela Proverbio§, DVM, PhD, Professor
Alessandra Della Pepa§, DVM
Paola Galluzzo*, Biologist
Roberta Perego§, DVM, PhD
Giada Bagnagatti De Giorgi§, DVM
Abstract
Feline vector-borne diseases are caused by a wide range of pathogens, which are transmitted by arthropods. Many of these infections have zoonotic implications and feral cats may potentially act as sentinels of human and pet health. The present study investigated the prevalence of vector-borne infections in feral colony cats in the city of Milan in northern Italy. Blood samples from 260 feral cats were evaluated, with conventional PCR, for the presence of DNA associated with hemoplasmas (Mycoplasma haemofelis and Mycoplasma haemominutum), Rickettsia spp., Anaplasma phagocytophilum, Ehrlichia spp. and Babesia microti. Odd ratios (OR) were calculated to identify risk factors for infection with vector-borne pathogens. Positive PCR was found in 156 out of 260 subjects (60%), with a prevalence of 33.1% for hemoplasmas, 31.9% for Rickettsia spp., 17.7% for A. phagocytophilum , 6.7% for Ehrlichia spp. (out of 30 samples), and 1.2% for B. microti spp (out of 168 samples). Statistical analysis revealed a correlation between infections with Rickettsia spp. and hemoplasmas (OR=1.95, P=0.02). Additionally, Rickettsia spp. infection was associated with ocular infection (OR=2.21, P=0.02). We conclude that vector-borne infections, including zoonotic diseases, are present in feral cats of Milan. Thus, domestic cats exposed to the outdoors should be routinely monitored and treated for ectoparasites to minimize disease onset and potential transmission of zoonotic agents to humans. Moreover, as these vector-borne infections are transmitted through blood, feline blood donors from this area should be screened by PCR
Variations on the Author
“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Prevalence of DEA 1 canine blood group in Italy
Background: DEA 1 canine group is the most immunogenic and it is considered the most clinically important to carry out blood transfusions.
Aims: To establish the prevalence of blood type DEA 1 in dogs belonging to several purebred and mongrel reared in Italy.
Methods: 7,414 pure-breed and mixed dogs retrieved from the database of the web site DogbloodDonors* recorded during the period October 2014-July 2016. DEA 1 blood type was identified mostly using the commercial in-clinic typing test RapidVetH Canine DEA 1.1, Agrolabo (Scarmagno, Turin, Italy).
Results: The DEA 1 frequency in all dogs studied is reported in figure 1. Mongrel dogs showed a distribution as depicted in figure 2.
In purebred dogs high prevalence for DEA 1 positive in Rottweiler (89.5%), Golden Retriever (75.2%), and Dachshund (74.2%) was observed. On the other hand German Shepherd, French Bulldog and West Highland White Terrier (WHWT) had a higher DEA 1 negative prevalence as found in previous studies.
There was a similar prevalence of DEA 1 in females and males as reported in table 1. Few exceptions were seen in Boxer female (DEA1+; 72.2%) and in Cavalier King Charles Spaniel and WHWT males (both with high DEA1-; 70.0%).
Conclusions: This study displays an overview of the prevalence of DEA 1 blood group in Italy that could be useful in transfusion medicine in order to look after breeds with low prevalence of DEA 1 positive dogs. The prevalence of DEA 1 positive and negative dogs agrees with most of the prevalence surveys reported in the literature
Anton Francesco Doni, La Zucca del Doni en Spañol (Venezia, Marcolini, 1551). Transcripción de Daniela Capra
El texto de la Zucca en Spañol que aquí presentamos fue impreso por Marcolini en Venecia en 1551. De la misma obra hemos publicado una edición crítica modernizada y anotada, a cuya introducción remitimos para toda información sobre la obra. Han quedado registradas en esta transcripción paleográfica unas erratas seguramente de imprenta y para que quede clara su atribución al tipógrafo de la oficina marcoliniana adjuntamos al final del texto una lista de erratas de este tipo con relativo número de página. Otras peculiaridades gráficas, como el uso o abuso de h y demás rasgos a veces comunes a otros autores de la época también se mantienen, pero sin señalarlo. Por la misma razón, se mantienen, sin señalar, vacilaciones de varia índole, como aʃi / asʃi / aʃsi / aʃʃi, esto / eʃto, o verʃos / uerʃos, vn / un, entre otras. También se ha respetado la puntuación, el uso de las mayúsculas, la separación o unión entre palabras, la colocación de la tilde que señala la palatalización de la nasal n, que el tipógrafo nunca (exceptuado el título en la portada) pone en la consonante, sino en la vocal que precede o sigue la n misma; asimismo, respetamos el salto de línea y la colocación, centrada, del proverbio y de los títulos de los capítulos
Anton Francesco Doni, La Zucca del Doni en Spañol (Venezia, Marcolini, 1551). Transcripción de Daniela Capra
El texto de la Zucca en Spañol que aquí presentamos fue impreso por Marcolini en Venecia en 1551. De la misma obra hemos publicado una edición crítica modernizada y anotada, a cuya introducción remitimos para toda información sobre la obra. Han quedado registradas en esta transcripción paleográfica unas erratas seguramente de imprenta y para que quede clara su atribución al tipógrafo de la oficina marcoliniana adjuntamos al final del texto una lista de erratas de este tipo con relativo número de página. Otras peculiaridades gráficas, como el uso o abuso de h y demás rasgos a veces comunes a otros autores de la época también se mantienen, pero sin señalarlo. Por la misma razón, se mantienen, sin señalar, vacilaciones de varia índole, como aʃi / asʃi / aʃsi / aʃʃi, esto / eʃto, o verʃos / uerʃos, vn / un, entre otras. También se ha respetado la puntuación, el uso de las mayúsculas, la separación o unión entre palabras, la colocación de la tilde que señala la palatalización de la nasal n, que el tipógrafo nunca (exceptuado el título en la portada) pone en la consonante, sino en la vocal que precede o sigue la n misma; asimismo, respetamos el salto de línea y la colocación, centrada, del proverbio y de los títulos de los capítulos
Imparare le lingue per abbracciare il mondo
L’autrice che si colloca, come Daniela Zorzi, tra i fautori di una linguistica “impegnata”, propone in ricordo della studiosa amica, una riflessione sul ruolo cruciale del multilinguismo e delle lingue- culture, per il successo professionale e il benessere sociale, nella società contemporanea. Il multilinguismo appare come un’urgenza educativa che interpella studiosi e formatori a tutti i livelli al fine di promuovere nei giovani un apprendimento consapevole efficace ed autonomo.Multilingualism constitutes an urgent objective for education, which needs to involve teachers and researchers at all levels if we are to promote effective and autonomous learning in the young. Like Daniela Zorzi, the author considers herself a socially engaged linguist, and in memory of her friend’s work, offers a reflection on the key roles of languages and cultures and of multilingualism for professional success and social welfare today
Effect of leukoreduction by pre-storage filtration on coagulation activity of canine plasma collected for transfusion
Leukoreduction of blood products is a technique used to prevent leukocyte-induced transfusion reactions and is extensively used in human, but rarely in veterinary patients. The concentration of some coagulation proteins can be affected by the processing steps used for the preparation of leuko-reduced plasma units. In this study, we assessed the effect of leukoreduction on coagulation activity of canine plasma collected for transfusion. Ten plasma units, five obtained from non-leuko-reduced (non-LR) whole blood (WB) units and five from leuko-reduced (LR) WB units were evaluated. Prothrombin time (PT), activated partial thromboplastin time (aPTT), coagulation factor activities of factors (F) V, VIII, X, XI, and von Willebrand (vWF), fibrinogen and D-dimers content were assessed at collection (baseline value, D0) and after 7 days of frozen storage at 18 C (D7). Compared to non-LR plasma units, LR units showed a statistically significant prolonged aPTT and reduced FXI activity. Filtration had no significant effect on the other factors and parameters evaluated. Filtration-dependent changes appear to have no impact on the therapeutic quality of plasma obtained from leuko-reduced whole blood, other than for FXI activity. Further studies on a larger sample size comparing the same unit before and after leukoreduction are needed to confirm these findings
Reclamações ambientais em Aveiro, Portugal: atores, preocupações, padrão territorial e resoluções
This paper presents an environmental diagnosis based on public complaints on environmental
issues submitted to the Environmental Department of the Aveiro City Council, Portugal, between
2000 and 2005. It discusses the potential influences of these in local environmental planning
and governance. The paper has been organised into five sections. The first of these introduces
the study. The second section focuses on the conceptual approaches relating to environmental
grassroots movements, the main actors involved in these movements and the role played by
local government. It also contains a brief review of the most recent urban environmental
quality challenges in the European context together with a description of the main features of
the associated political and legal framework in Portugal. The third section describes the case
study and the methodology used. The results of the empirical study are detailed in the fourth
section. The final section critically analyses these results with emphases on the temporal
evolution of the submission of complaints, the actors involved, the local environmental problems
and their associated spatial pattern as well as the responses given by the City Council. This
information may then be used to provide a useful indicator for the perception of environmental
quality as well as a credible instrument for the visualisation and evaluation of local performance
in terms of environmental planning and management.AlBan Programme - n. E05M053040B
The card agglutination test for dog erythrocyte antigen 1 (DEA 1) blood typing in donor dogs: Determining an appropriate cutoff to detect positivity using a receiver operating characteristic curve
Background The appropriate cutoff to define a positive point-of-care card agglutination (CA) test for dog erythrocyte antigen 1 (DEA 1) blood typing depends on whether the test can be used in the donor or recipient. Objectives By screening for CA test positivity, we aimed to evaluate the best cutoff value for DEA 1 blood typing in canine blood donors using a receiver operator characteristic (ROC) curve. Methods Ethylenediaminetetraacetic acid (EDTA) blood samples from 100 canine blood donors were blood-typed in parallel for DEA 1 using both immunochromatographic (IC) and CA tests. The effect of temperature, storage time, and anticoagulant solutions for both methods was evaluated. Unweighted and weighted Cohen's Kappa (K) statistic was calculated to evaluate the agreement between the two testing methods. The overall performance of the CA test was evaluated by generating a ROC curve using the IC test as the reference method. Results Concordant results were obtained for 86% of the samples. Unweighted and weighted K statistics demonstrated good and moderate agreement, respectively. For the CA test, the ROC curve showed an area under the curve (AUC) of 0.910, with the highest sensitivity cutoff values at >= 1+ agglutination. CA- and IC-typed EDTA blood samples stored at room temperature for up to 1 week and refrigerated for up to 1 month were concordant as were the citrate phosphate dextrose adenine 1 (CPDA-1) anticoagulated blood samples stored for up to 1 week at 4 +/- 2 degrees C. Conclusions The overall reliability of the CA method seemed to be lower than that of the IC method. When CA is used as a screening test for canine blood donors, the correct cut off is >= 1+ agglutination is recommended to maximize sensitivity
Critically Appraised Topic on Low-Level Laser Therapy (LLLT) in Dogs: An Advisable Treatment for Skin Diseases?
Low-level laser therapy (LLLT) is a therapeutic option that stimulates cellular function through intracellular photobiological and photochemical reactions, promoting better tissue repair and an anti-inflammatory, antibacterial, and analgesic effect. Previous studies in human and veterinary medicine have shown the clinical efficacy of LLLT in many fields. In this study, the literature was reviewed using the critically appraised topic (CAT) method to determine the canine skin diseases for which LLLT is an advisable treatment. A meticulous literature search revealed 19 significant clinical trials, which were critically analyzed. The evaluation of the best accessible evidence in July 2022 suggests that fluorescence biomodulation (FBM), a type of LLLT, can, in combination with systemic antibiotic therapy, be a promising and effective adjunctive treatment for canine interdigital pyoderma and canine deep pyoderma. Furthermore, the evidence suggests that the use of LLLT is not recommended as a therapy for pedal pruritus secondary to canine atopic dermatitis. For other canine skin diseases included in the CAT, although LLLT appears to be a promising treatment, there is not yet good scientific evidence to recommend its use
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