94 research outputs found
Role of the JP45-Calsequestrin Complex on Calcium Entry in Slow Twitch Skeletal Muscles
No full textWe exploited a variety of mouse models to assess the roles of JP45-CASQ1 (CASQ, calsequestrin) and JP45-CASQ2 on calcium entry in slow twitch muscles. In flexor digitorum brevis (FDB) fibers isolated from JP45-CASQ1-CASQ2 triple KO mice, calcium transients induced by tetanic stimulation rely on calcium entry via La3+- and nifedipine-sensitive calcium channels. The comparison of excitation-coupled calcium entry (ECCE) between FDB fibers from WT, JP45KO, CASQ1KO, CASQ2KO, JP45-CASQ1 double KO, JP45-CASQ2 double KO, and JP45-CASQ1-CASQ2 triple KO shows that ECCE enhancement requires ablation of both CASQs and JP45. Calcium entry activated by ablation of both JP45-CASQ1 and JP45-CASQ2 complexes supports tetanic force development in slow twitch soleus muscles. In addition, we show that CASQs interact with JP45 at Ca2+ concentrations similar to those present in the lumen of the sarcoplasmic reticulum at rest, whereas Ca2+ concentrations similar to those present in the SR lumen after depolarization-induced calcium release cause the dissociation of JP45 from CASQs. Our results show that the complex JP45-CASQs is a negative regulator of ECCE and that tetanic force development in slow twitch muscles is supported by the dynamic interaction between JP45 and CASQs
The amoebal MAP kinase response to Legionella pneumophila is regulated by DupA
No full textThe amoeba Dictyostelium discoideum can support replication of Legionella pneumophila. Here we identify the dupA gene, encoding a putative tyrosine kinase/dual-specificity phosphatase, in a screen for D. discoideum mutants altered in allowing L. pneumophila intracellular replication. Inactivation of dupA resulted in depressed L. pneumophila growth and sustained hyperphosphorylation of the amoebal MAP kinase ERK1, consistent with loss of a phosphatase activity. Bacterial challenge of wild-type amoebae induced dupA expression and resulted in transiently increased ERK1 phosphorylation, suggesting that dupA and ERK1 are part of a response to bacteria. Indeed, over 500 of the genes misregulated in the dupA(-) mutant were regulated in response to L. pneumophila infection, including some thought to have immune-like functions. MAP kinase phosphatases are known to be highly upregulated in macrophages challenged with L. pneumophila. Thus, DupA may regulate a MAP kinase response to bacteria that is conserved from amoebae to mammals
Guideline for food waste reduction in Hospitality Industry-Study Case: Hotel F6, Helsinki
No full textFood waste remains a problem in the food industry since it is caused by a variety of sources and is difficult to eliminate it in food production. It causes environmental and economic consequences. The hotel and food service industry playing a large part in the process of generating food that is discharged into the environment at the consumption stage. Therefore, it is critical to have a substantial potential for food waste avoidance in this sector.
The objective of this thesis is to provide guidelines for reducing food waste in the hospitality industry based on research of definition of food loss and food waste, the causes of food waste, the impacts of food waste, and current approaches and practices for food waste management in the food and beverage industry. This topic is addressed in this thesis on a limited scale, with the study case of F6 hotel. The commission was given on April 2020 and the thesis is mainly conducted and finished in May 2022
The primary approach for the study was qualitative research. To provide an in-depth analysis of the F6 hotel’s food waste management system, qualitative research was used. The primary data collection approach were observation and theme interviews. Observation is carried on based on structured observation form. Theme interviews were conducted with F6 hotel workers in order to provide an overview of food waste management.
In this thesis, the author will provide systematic instructions on how to minimize food waste in F6 hotel in four distinctive categories: daily food waste tracking, inventory control, staff training and raise customer awareness. One of the suggestions given in the guidelines is to check storage temperatures regularly, use the FIFO method, or improve staffs cutting and trimming skills. Overall, results are given out matching up with the studied theory and the objectives of commissioner
For the Union Dead
No full textNew York, Farrar, Straus & Giroux, 1964
Call #: PS3523.O89 F6
Inscribed by author and contained ephemera found inside front cover: Robert Lowell’s poem, “Waking Early Sunday Morning,” torn from August 5, 1965 issue of the New York Review of Books. Arendt ephemera file; folder 008.
PDF Information: 2 pages – 338 KBhttps://digitalcommons.bard.edu/hapl_marginalia_all/1381/thumbnail.jp
Poor relief and welfare work in Fort Collins, Colorado: with an introduction giving the development of poor relief down to the present
No full textMay 24, 1932.Includes bibliographical references (page 182)
Bioreactor Feeding Strategies to Improve Biogas Production and Pig Slurry Management Flexibility
No full textThis work was supported by Fundação para a Ciência e a Tecnologia (FCT) through the strategic project UID/04129/2020 of LEAF and the PhD grant to the first author (DOI: 10.54499/2023.03381. BD).
Publisher Copyright:
© (2024), (Polskie Towarzystwo Inzynierii Ekologicznej (PTIE)). All rights reserved.Pig slurry (PS) management is a challenge that needs appropriate strategies. Anaerobic digestion (AD) has proved to be an interesting option to follow when it comes to livestock effluent management. Although this technology is well established, it is crucial to investigate different scenarios that demonstrate its suitability to motivate farmers to adopt new strategies for PS management. Previous research, based on a daily feeding regime and a 2-day starvation-induced period, investigated the impact of feast/famine cycles on the AD process. There was evidence of a positive link between AD performance and the starvation regime. From this assumption, new scenarios were designed combining different feeding frequencies: a) one daily feeding (F1) and six feedings per day (F6), b) two days of starvation with one feeding (S1) and with six feedings (S6). The operational parameters were settled in advance: organic loading rate (1.5±0.2 g VS/Lreactor.d), hydraulic retention time (15 days), and mesophilic conditions (37±1 ºC). The results obtained in this work indicate a significant improvement (P < 0.05) of 92% in specific methane production when comparing the trial F1 with F6. VS reduction remained constant in F1 and F6, but the starvation period (S1) led to an increase in VS reduction compared to both F1 (27%) and F6 (33%). The results obtained are in agreement with the previous work conducted by the authors. This study highlights how feeding frequency and starvation affect biogas production, assessing their effectiveness on biogas yield. This tool will give farmers a key decision factor to make an evidence-based decision and can work as a contingency planning strategy, aligning their PS management needs with AD versatility.publishersversionpublishe
Aerodynamic Shape Optimization Using the Discrete Adjoint of the Navier-Stokes Equations: Applications Toward Complex 3D Configutations
No full textWithin the next few years, numerical shape optimization based on high fidelity methods is likely to play a strategic role in future aircraft design. In this context, suitable tools have to be developed for solving aerodynamic shape optimization problems, and the adjoint approach - which allows fast and accurate evaluations of the gradients with respect to the design parameters - is seen as a promising strategy. After describing the theory of the viscous discrete adjoint method and its implementation within the unstructured RANS solver TAU, this paper describes application for aerodynamic shape optimization. First wing and fuselage designs of the DLR-F6 wing-body aircraft are presented. A step forward in complexity is considered by applying the adjoint for flap and slat optimal settings of the DLR-F11 model, a wing-body aircraft in high-lift configuration. On all cases presented, optimization were successfully performed within a limited number of flows evaluations
akym/SX3-Calibration: Teensy program for SX3 calibration control box - Alpha
No full textSX3-Calibration
<p>SX3 Thrust Calibration and measurement Program for Teensy 3.6</p>
Usage Instruction
<p>Download this file and compile 'sx3_cal.ino' using Arduino IDE and upload to Teensy via Teensy loader. Make sure you have the support libraries from <a href="https://zenodo.org/badge/latestdoi/133398390"></a>.</p>
Current Function Mapping
Switch
Function
F1
Lock thrust balance
F2
Decouple thrust balance
F3
Calibrate
F4
Preload Force Sensor
F5
Deload Force Sensor
F6
Stop Preload/Deload
Author
<p>Akash Mankar</p>
The Fourth Kentucky Volunteer Infantry by W. P. Norris (E726 .K37 F6)
No full textThe Fourth Kentucky Volunteer Infantry by W.P. Norris (S.l.: The Author, 19--) outlines the regiment\u27s history and includes a roster of officers and soldiers. A full-text scan of this book provided by Library Special Collections at WKU can be seen by cutting and pasting this url into your browser: https://digitalcommons.wku.edu/cgi/viewcontent.cgi?article=1176&context=dlsc_kl_non_mathttps://digitalcommons.wku.edu/tlw_secondary/1006/thumbnail.jp
Extraction yield optimization of Oleaster (Olea europaea var. sylvestris) fruits using response surface methodology, LC/MS profiling and evaluation of its effects on antioxidant activity and autophagy in HFF cells
No full textOleaster (Olea europaea var. sylvestris) is a wild olive tree distributed in the Mediterranean area. It is known to possess several biological activities due to their richness in polyphenols. However, enhanced autophagic activity in human cells by using this plant has not been studied before. The objective of the present study was to determine the phytochemical composition of the fruits paste from Tunisian Oleaster, and evaluate its antioxidant activity using the DPPH• and ABTS•+ methods as well as the evaluation of its effect on autophagy activity. The extraction conditions were optimized using response surface methodology (RSM) leading to the best phenolic extract yield. It was noted that ethyl acetate extract presented the highest amount of total phenols and the best solvent for extraction of phenolic compounds. After optimization, the ethyl acetate extract was fractionated into 15 fractions by silica gel column chromatography. The most phenolic components in the fifth and sixth fractions (F5 and F6) were identified using UPLC-ESI(-)-HRMS analysis. Accordingly, F6 was the richer fraction in polyphenols. In vitro antioxidant activity evaluation showed that F6 exhibited a strong ability to scavenge DPPH and ABTS radicals. Furthermore, this fraction was also the most effective to induce autophagic activity in Human foreskin fibroblasts (HFF) cells. These results could be presumably due to the presence of oleuropein aglycon in this fraction which is absent in fraction F5. Oleaster fruits paste would be used as a natural source of bioactive compounds useful in various pharmaceutical drugs. © 2021, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature
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