568 research outputs found
Modification of Loop 1 Affects the Nucleotide Binding Properties of Myo1c, the Adaptation Motor in the Inner Ear
No full textMyo1c is one of eight members of the mammalian myosin I family of actin-associated molecular motors. In stereocilia of the hair cells in the inner ear, Myo1c presumably serves as the adaptation motor, which regulates the opening and closing of transduction channels. Although there is conservation of sequence and structure among all myosins in the N-terminal motor domain, which contains the nucleotide- and actin-binding sites, some differences include the length and composition of surface loops, including loop 1, which lies near the nucleotide-binding domain. To investigate the role of loop 1, we expressed in insect cells mutants of a truncated form of Myo1c, Myo1c1IQ, as well as chimeras of Myo1c1IQ with the analogous loop from other myosins. We found that replacement of the charged residues in loop 1 with alanines or the whole loop with a series of alanines did not alter the ATPase activity, transient kinetics properties, or Ca2+ sensitivity of Myo1c1IQ. Substitution of loop 1 with that of the corresponding region from tonic smooth muscle myosin II (Myo1c1IQ-tonic) or replacement with a single glycine (Myo1c1IQ-G) accelerated the release of ADP from A.M 2?3-fold in Ca2+, whereas substitution with loop 1 from phasic muscle myosin II (Myo1c1IQ-phasic) accelerated the release of ADP 35-fold. Motility assays with chimeras containing a single ?-helix, or SAH, domain showed that Myo1cSAH-tonic translocated actin in vitro twice as fast as Myo1cSAH-WT and 3-fold faster than Myo1cSAH-G. The studies show that changes induced in Myo1c via modification of loop 1 showed no resemblance to the behavior of the loop donor myosins or to the changes previously observed with similar Myo1b chimeras
Jugoslovenija: Slavljenje nekdanjih jugoslovanskih praznikov v sodobni Sloveniji, 2013
No full textRaziskava obravnava kulturne prakse in razsežnosti ter ideološke pomene nekdanjih treh najbolj priljubljenih jugoslovanskih praznikov v sodobni Sloveniji. To so Dan mladosti (25. maj), Dan republike (29. november) in obletnica smrti Josipa Broza Tita (4. maj). Namen je bil ugotoviti, v kakšni obliki se te tri nekdanje praznike proslavlja danes ter kakšne pomene in politične posledice imajo ti prazniki v sodobni slovenski družbi. Znotraj tega opisa raziskave je dostopno slikovno in tekstovno gradivo, ki ga je avtor uporabil v svoji raziskavi in služi kot dodatek k poglobljeni analizi drugih vsebin o omenjenih praznikih, ki jih avtor navaja v članku »Jugoslovenija: Slavljenje nekdanjih jugoslovanskih praznikov v sodobni Sloveniji«, kjer temeljito predstavi pojav obeleževanja dogodkov v Sloveniji po osamosvojitvi.The study deals with cultural practices, dimensions, and ideological meanings of the three most popular former Yugoslav holidays in contemporary Slovenia. These are the Youth Day (25 May), the Day of the Republic (29 November) and the Anniversary of the Death of Josip Broz Tito (4 May). The purpose of the study was to determine, in what form these three former holidays are being celebrated today, and what kind of meanings and political consequences they have in the contemporary Slovene society. Within the study description, only a few pictures (n=9) and textual materials (n=29) that were used by the author in his research as a supplement to an in-depth analysis of other content regarding holidays mentioned by the author within the article »Jugoslovenija: Slavljenje nekdanjih jugoslovanskih praznikov v sodobni Sloveniji«, where he thoroughly presents the occurrence of events in Slovenia after its independence, are available
Inovacijske dejavnosti slovenskih podjetij v gozdno-lesni verigi, 2019
No full textSlovenija velja za regijo, ki zaostaja za inovacijami, kar je še posebej opazno v gozdno-lesni verigi. Hkrati pa v okviru slovenske strategije pametne specializacije poudarjajo, da ima ta panoga velik potencial za rast. Da bi lahko kar najbolje izkoristili inovacijski potencial gozdno-lesne verige, je potrebno najprej poglobljeno razumeti obstoječe inovacijske dejavnosti in razloge za njihovo pomanjkanje. Na ravni EU podatke o inovacijskih dejavnostih zbira Eurostatova anketa o inovacijskih dejavnostih v industriji in izbranih storitvenih dejavnostih (Community Innovation Survey, CIS), ki se vsaki dve leti izvaja od leta 2006. Vendar raziskava vključuje samo podjetja z 10 ali več zaposlenimi. Ker mikro podjetja predstavljajo več kot 90 % podjetij v slovenski gozdno-lesni verigi, so za ustrezno razumevanje inovativnosti v tem sektorju nujni podatki o njihovih inovacijskih dejavnostih. Pričujoča raziskava zajema tržne in inovacijske dejavnosti slovenskih podjetij v gozdno-lesnem sektorju, pri čemer so bila zajeta podjetja vseh velikosti. Raziskava se nanaša na obdobje 2016-2018. Raziskava vsebuje dve podatkovni datoteki, in sicer datoteko, ki vsebuje parapodatke in je dostopna pod posebnimi pogoji t. j. na zahtevo preko varne povezave (SUF verzija) in zajema dva vira podatkov - avtorske podatke in podatke iz drugih virov (Bizi.si – poštni okraj, pravna oblika, število zaposlenih in SKD klasifikacija) ter datoteko s podatki na osnovi vprašalnika, ki je dostopna ob predhodni registraciji (ter dodatna datoteka v .xlsx formatu, z anonimiziranimi odgovori dveh odprtih vprašanj).Slovenia was identified as a region lagging in innovation and this is particularly noticeable in the country’s forest-based value chain. However, Slovenia’s Smart Specialisation Strategy identified this industry as having a strong potential for growth. To leverage the innovation and growth potential of the forest-based value chain, we need to gain a deeper understanding of existing innovation activities and reasons for the lack of innovation activities. On the EU level, data on innovation activities are collected by the Community Innovation Survey (CIS), which has been carried out every two years since 2006. However, the survey only includes companies with 10 or more employees. Since micro companies represent more than 90% of forest-based value chain companies in Slovenia, data on their innovation activities is needed to fully understand it. The present research covers the market and innovation activities of Slovenian companies in the forest-timber sector, whereby companies of all sizes were covered. Research covers the period 2016-2018. The research contains two data files, that is file containing the paradadata, accessible under special conditions viz. on request via a secure connection (SUF version), which includes two types of data sources – one created by author and other imported from other sources (Bizi.si registry - postal district, legal form, number of employees and NACE code) and questionnaire-based data file that is accessible upon prior registration (and an additional file in .xlsx format, with anonymized answers to two open questions)
Kakovost bivalnega okolja v izbranih slovenskih mestih, 2014
No full textOsrednji namen raziskave je bil izmeriti kakovost bivalnega okolja v izbranih mestnih območjih izbranih slovenskih mest na začetku drugega desetletja 21. stoletja na subjektiven način, to je z vidika vrednotenja s strani tamkajšnjih prebivalcev. Kakovost bivalnega okolja je bila ocenjena v skupno 15 soseskah različnih morfoloških tipov v petih slovenskih mestih: Ljubljani, Mariboru, Kopru, Jesenicah in Grosuplju, ki ponazarjajo pet različnih tipov mest glede na velikost, družbenogospodarske značilnosti, položaj v urbanem sistemu in naravnogeografske značilnosti. Raziskava je bila izvedena v tamkajšnjih starih mestnih središčih ter izbranih območjih pretežno enodružinskih hiš, starejših blokovskih sosesk in sodobnih večstanovanjskih hiš. Na poti k uresničitvi namena smo si zadali naslednje cilje: opredelitev pojma bivalno okolje, njegovih prostorskih ravni in glavnih vsebin, ocena kakovosti posameznih vsebin bivalnega okolja, ocena kakovosti bivalnega okolja kot celote, razkritje dejavnikov ocen kakovosti bivalnega okolja, ugotovitev potencialne selitvene mobilnosti, prepoznavanje bivalnih preferenc prebivalstva in ovrednotenje pomena bivalnega okolja kot ene izmed vsebin kakovosti življenja. Avtor je analizo izvedel v izbranih soseskah.The main purpose of this research was to estimate the quality of the residential environment in the selected Slovenian cities in the early twenty-first century with regards to the subjective assessment of the inhabitants. The quality of the residential environment was studied in Ljubljana, Maribor, Koper, Jesenice, and Grosuplje, which represent five different types of urban environments with regards to size, socio-economic characteristics, urban centrality, and the physical geographical characteristics, in their fifteen neighborhoods of distinctive morphological types: areas of single-family houses, historical city centres, neighborhoods of post-WWII older apartment buildings, areas of contemporary apartment buildings. In order to realise the purpose, the following goals were set: to define the term residential environment and determine its spatial levels and main elements, to estimate the quality of individual elements of the residential environment, to estimate the quality of the residential environment as a whole, to reveal the most important factors of the quality of residential environment, to estimate the desire to move, to reveal the residential preferences of the inhabitants and to estimate the importance of the residential environment as one of the quality of life domains. The author carried out the analysis by selected neighbourhoods
Odprava administrativnih ovir, 2014
No full textPri pripravi raziskave so bila glavna izhodišča usmerjena v alternativno koncepcijo ideološko obremenjenega konstrukta administrativnih ovir in raziskava percepcije podjetij kot ključnih deležnikov, ki s svojim razumevanjem, vedenjem in zavedanjem lahko prispevajo k oblikovanju ukrepov za izboljšanje zakonodajnega in poslovnega okolja. Namen raziskave »Odprava administrativnih ovir« je bil pridobiti kategorizacijo administrativnih ovir ter ugotoviti seznanjenost z ukrepi za odpravo administrativnih ovir. Cilj raziskave je bila konceptualizacija besedne zveze administrativna ovira, ki je nadalje služila tudi kot pomoč pri terminološki klarifikaciji javnopolitičnega področja. V raziskavi je bila pozornost namenjena tudi razjasnitvi tipa bremena (časovno, finančno in ostale oblike), ki ga administrativne ovire povzročajo podjetjem. Raziskava sledi trendom na področju raziskovanja percepcije podjetij in njihovega dojemanja regulacije ter njenih posledic. V mednarodnem okolju tovrstne raziskave dopolnjujejo surove podatke o (ne)uporabi regulatornih mehanizmov in pomenijo doprinos k identifikaciji realnega stanja na področju določene javne politike. Del vsebine vprašalnika in podatkovne datoteke je vezan na poslovno skrivnost in je na vpogled pri avtorju raziskave.The study is focused on an alternate conception of an ideologically burdened administrative burden concept as well as a perception survey of key stakeholders – business owners. The main idea was to enhance stakeholders comprehension, knowledge and awareness so that they could in effect further improve the public policy of administrative burden reduction. The purpose of the research »Cutting red tape« was to categorize administrative burdens and to establish how key stakeholders are familiar with administrative burden reduction measures. The purpose was also to conceptualize the term administrative burden, which served later as an important determinant in the terminological clarification of a policy area. The research also focused on the different types of burden (temporal, financial and other types) that businesses endure due to the existence of administrative burdens. The research follows trends in the area of business perception surveys and their comprehension of the regulation and its effects. In the international environment such research complement raw data about the (dis)use of regulatory mechanisms and represent a valuable contribution to identification of a certain public policy. Part of the content of the questionnaire and data file is bound to business secret and is available from the author of the survey
Concorso Internazionale ad inviti "MEP Projet Pôle Gare", Losanna
No full textLe programme des CFF Léman 2030 va transformer la gare de Lausanne et ses interfaces afin de doubler sa capacité d’accueil. Cette transformation agira sur le coeur de l’agglomération comme un véritable détonateur du développement urbain. Dans ce contexte, l’aménagement des espaces publics devra être revu au Nord comme au Sud des voies. La première étape porte sur le projet de réaménagement de la Place de la Gare et un concept directeur des espaces publics du Pôle Gare.
Pour développer cette étude, la ville a choisi la procédure du mandat d’études parallèles (MEP). 32 groupements ont répondu à l’appel à candidatures pour ce MEP, lancé en mars 2015. Sur recommandation d’une délégation du jury, présidé par Monsieur Olivier Français, municipal en charge de la Direction des travaux, la Municipalité a finalement retenu les six groupements suivants.
- Caruzo St john Architects - Caruso St John Architects avec Antòn & ghiggi (pilote), E. Ferrari Ingénieure (ing. civil), IBV Hüsler (ing. mobilité) ; Zurich, Suisse.
- TVK / B+S AG / RR&A /BSAU /OLM - Trévelo & Viger-Kohler architectes urbanistes (pilote), B+S AG (ing. civil), Roland Ribi & Associés Sàrl Strasbourg (ing. Mobilité), BSAV / Blaise Sahy (archi-urba), OLM / Philippe Coignet (paysagiste) ; Paris, France.
- Privilieggio Secchi Architecture – Arch. Nicolò Privilieggio (pilote), MG Progetti, studio di ingneria (ing. civil), Ing. Luca della Lucia (ing. mobilité) ; Milan, Italie.
- MSV + B&H – MSV architectes urbanistes (pilote), Basler & Hofmann AG (ing. civil +mobilité), Van de Wetering atelier d’urbanisme (expert urbaniste), François Gschwind (concepteur lumière) ; Les Acacias (GE), Suisse.
- Pool 2b / adP / MFIC / IDTech / mrs – 2b architectes (architecte) (pilote), l’Atelier du paysage Jean-Yves le Baron (paysagiste) (pilote), Muttoni Fernandez ingénieurs conseils (ing. civil), iDTech (ing. civil), mrs Partner (ing. mobilité) ; Lausanne, Suisse.
- Equipe Hüsler & Associés + KCAP – Hüsler & Associés Sàrl (pilote), KCAP Architects & Planers (archi-urba), MCR & Associés Sàrl (ing. civil), Buchhofer AG (ing. Mobilité), d-lite (éclairagiste) ; Lausanne, Suisse
Nicolas Floc'h "Un parcours, une démarche, une séance"
No full textCompte-rendu de séance "Parcours et démarche artistique" de Nicolas FLOC'H (artiste plasticien) du mardi 11 décembre 2012, Centre chorégraphique National de Franche-Comté à Belfort par Jennifer BONN LOMBARD Nicolas Floc’h, Performance painting #2, 2005 © Adagp, Paris 2011 Le parcours artistique de Nicolas Floc'h est un apprentissage multi-directionnel impulsé par une curiosité vive qui le guide de projet en projet. Chaque nouvelle pièce est l'occasion d'une découverte et d'une profonde reche..
Prepoznavanje ravni jezikovne zmožnosti romskih otrok in učencev, 2023
No full textNamen in cilj raziskave je bil razvoj instrumentarija in testiranje ravni jezikovne zmožnosti otrok v romskem in slovenskem jeziku. S testiranjem se je pridobilo osnovni vpogled v jezikovno zmožnost romskih otrok v predšolskem obdobju in v prvi triadi OŠ. V ta namen je bil uporabljen prilagojen (zamenjava pravljice) in preveden (v obe obliki testiranih narečij) instrumentarij (Nećak Lük, A., 1989. Vzgoja in izobraževanje v večjezičnih okoljih. Motivacija za učenje maternega in drugega jezika v dvojezični šoli v Prekmurju. Pedagoški inštitut, Ljubljana), medtem, ko je bil slikovni material pridobljen z CanvaPro licenco (https://www.canva.com/). Jezikovna zmožnost romskih otrok je eden od pogojev za zagotavljanje vključujočega in pravičnega izobraževalnega sistema. S krepitvijo jezikovnih zmožnosti romskih otrok v njihovem maternem jeziku se povečujejo možnosti za ohranjanje njihove lastne kulturne identitete, za doseganje večje uspešnosti v šoli, nadaljevanje šolanja in pridobitev izobrazbe. Z instrumentarijem zbrani podatki bodo služili kot izhodiščno gradivo pri oblikovanju učnih programov za romščino kot J1, potencialno pa tudi kot J2. Poznavanje izhodiščnega jezikovnega repertoarja v romskem J1 pa bo služilo tudi kot podlaga za načrtovanje ustreznih didaktičnih pristopov in postopkov pri pouku slovenščine kot učnega jezika in kot učnega predmeta. Prvič v zgodovini raziskovanja romščine v Sloveniji bo pridobljen bazični jezikovni korpus razvitosti romščine v predformalnem, operativnem stadiju. Prilagojene različice instrumenta bodo uporabne pri preverjanju jezikovne zmožnosti otrok iz drugih manjšinskih ali migrantskih skupnosti. Instrumentarij bo tako osnova za ustrezen monitoring pri jezikovno-političnih ukrepih za razvoj sporazumevalne zmožnosti pripadnikov romske skupnosti. Za razumevanje poteka zbiranja in samih podatkov glej dokument "Prepoznavanje ravni jezikovne zmožnosti romskih otrok in učencev, 2023. Kodirnik". Osnova za pripravo podatkovne datoteke so bili pridobljeni podatki na obrazcih jezikovnega testa in transkripti (obrazec za transkribiranje), narejeni na podlagi avdio posnetkov testiranj. Celotni transkripti so sicer dostopni za raziskovalce, pri čemer se je za dostop potrebno obrniti na avtorja raziskave.The purpose and objective of the research was to develop an instrument and to test the level of linguistic competence in Romani and Slovene language among Romani children. Through testing a basic insight into the linguistic competence of children in the pre-school period and in the first three years of primary school was gained. For this purpose, an adapted (replaced fairy tale) and translated (in both tested dialects) instrument (Nećak Lük, A., 1989. Vzgoja in izobraževanje v večjezičnih okoljih. Motivacija za učenje maternega in drugega jezika v dvojezični šoli v Prekmurju. Pedagoški inštitut, Ljubljana), was used. The image material was obtained with a CanvaPro license (https://www.canva.com/). The linguistic competence of Romani children is one of the conditions for ensuring an inclusive and equitable educational system. Strengthening the linguistic competences of Romani children in their mother tongue increases their chances of preserving their cultural identity, doing better at school, continuing schooling, and obtaining an education. The data collected with the instrument will serve as a basis for the design of educational programmes for Romani as L1 and potentially also as L2. The knowledge of the initial linguistic repertoire in Romani L1 will also serve as a basis for planning the necessary didactic approaches and procedures for teaching Slovene as a language of instruction and as a subject. For the first time in the history of Romani language studies, a baseline linguistic corpus of the development of Romani at the pre-formal, operational stage will be created. Adapted versions of the instrument will be useful in testing the linguistic competence of children belonging to other minority or migrant communities. The instrument will thus serve as a basis for monitoring language-policy measures aimed to develop the communicative competence of members of the Romani community. To understand the collection process and data itself see the document "Prepoznavanje ravni jezikovne zmožnosti romskih otrok in učencev, 2023. Kodirnik" (Coodebook). The basis for preparing the data file was the data obtained from the language test forms and transcripts (transcribing form) made based on audio recordings of the tests. The full transcripts are available to researchers, but for access you must contact the author of the research
Characterization of ADP-glucose transport across the cereal endosperm amyloplast envelope
No full textMost of the carbon used for starch biosynthesis in cereal endosperms is derived from ADP-glucose (ADP-Glc) synthesized by extra-plastidial AGPase activity, and imported directly across the amyloplast envelope. The properties of the wheat endosperm amyloplast ADP-Glc transporter were analysed with respect to substrate kinetics and specificities using reconstituted amyloplast envelope proteins in a proteoliposome-based assay system, as well as with isolated intact organelles. Experiments with liposomes showed that ADP-Glc transport was dependent on counter-exchange with other adenylates. Rates of ADP-Glc transport were highest with ADP and AMP as counter-exchange substrates, and kinetic analysis revealed that the transport system has a similar affinity for ADP and AMP. Measurement of ADP and AMP efflux from intact amyloplasts showed that, under conditions of ADP-Glc-dependent starch biosynthesis, ADP is exported from the plastid at a rate equal to that of ADP-Glc utilization by starch synthases. Photo-affinity labelling of amyloplast membranes with the substrate analogue 8-azido-[α-32P]ADP-Glc showed that the polypeptide involved in substrate binding is an integral membrane protein of 38 kDa. This study shows that the ADP-Glc transporter in cereal endosperm amyloplasts imports ADP-Glc in exchange for ADP which is produced as a by-product of the starch synthase reaction inside the plastid. © 2007 The Author(s)
Synthesis of Cyclic ADP-Carbocylcic-Xylose and its 3′′-OO-Methyl Analogue as Stable and Potent Ca2+-Mobilizing Agents
No full textWe previously showed that 3”-deoxy-cyclic ADP-carbocyclic-ribose (3”-deoxy-cADPcR, 2) is a stable and highly potent analogue of cyclic ADP-ribose (cADPR, 1), a Ca2+-mobilizing second messenger. From these results, we newly designed another 3”-modified analogues of cADPcR and identified the N1-“xylo”-type carbocyclic analogue, i.e., cADPcX (4), as one of the most potent cADPR-related compounds reported so far
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