1,064 research outputs found
Membrane protein production and purification from <i>Escherichia coli</i> and <i>Sf9</i> insect cells
Full text linkA major obstacle to studying membrane proteins by biophysical techniques is the difficulty in producing sufficient amounts of materials for functional and structural studies. To overexpress the target membrane protein heterologously, especially an eukaryotic protein, a key step is to find the optimal host expression system and perform subsequent expression optimization. In this chapter, we describe protocols for screening membrane protein production using bacterial and insect cells, solubilization screening, large-scale production, and commonly used affinity chromatography purification methods. We discuss general optimization conditions, such as promoters and tags, and describe current techniques that can be used in any laboratory without specialized expensive equipment. Especially for insect cells, GFP fusions are particularly useful for localization and in-gel fluorescence detection of the proteins on SDS-PAGE. We give detailed protocols that can be used to screen the best expression and purification conditions for membrane protein study
Education and Training in St. Vincent and the Grenadines: A Partially Annotated Bibliography
Full text linkThis bibliography on “Education and Training in St. Vincent and the Grenadines” has been specifically prepared for the UWI School of Continuing Studies’ St. Vincent and the Grenadines Conference. It covers all aspects of education and training in St. Vincent and the Grenadines including: Academic achievement,economics of education, educational infrastructure, literacy and mathematics education
Autoworker and acclaimed author Ben Hamper speaks at the Michigan Writers Series
No full textIn an appearance at the Michigan State University Main Library, autoworker and acclaimed author Ben Hamper talks about his career at the General Motors Truck and Bus Plant in Flint, Michigan and reads from various works, including his forward to the book "Working words: punching the clock and kicking out the jams" by M. L. Liebler and from his most famous work, "Rivethead", a cynical and humorous view of life in an auto plant. A question and answer session follows. Hamper is introduced by Michigan State University Professor John P. Beck for the Michigan State University Libraries' Michigan Writers Series
Jon Frey, Michigan State University professor of Art and Art History talks about the reuse of building materials in late Roman and Byzantine buildings and into the Medieval period
No full textJon Frey, Michigan State University professor of Art and Art History and author of "Spolia in fortifications and the common builder in late antiquity", talks about the reuse of building materials in late Roman and Byzantine buildings and into the Medieval period, especially those elements with Latin or Greek inscriptions. Frey talks about literacy rates during those periods and asks if the elements were actually meant to be read or if it was sufficient to the builder that they be recognized simply as ancient writing. Frey is introduced by the Head of the Fine Arts Library, Terrie Wilson
Stephanie Mathson interviews essayist and memoirist Robert Root
No full textEssayist and memoirist Robert Root, professor of English at Central Michigan University, talks about his book "Recovering Ruth" and the genealogical research research in his work and his role as both a university professor and an author. He also shares his views on creative nonfiction, Michigan as a source of inspiration, and works in progress. Root is interviewed by Stephanie Mathson of the Michigan State University Libraries for the MSU Libraries' Michigan Writers Series
The use of SMALPs as a novel membrane protein scaffold for structure study by negative stain electron microscopy.
Full text linkDespite the great progress recently made in resolving their structures, investigation of the structural biology of membrane proteins still presents major challenges. Even with new technical advances such as lipidic cubic phase crystallisation, obtaining well-ordered crystals remains a significant hurdle in membrane protein X-ray crystallographic studies. As an alternative, electron microscopy has been shown to be capable of resolving >3.5Å resolution detail in membrane proteins of modest (~300kDa) size, without the need for crystals. However, the conventional use of detergents for either approach presents several issues, including the possible effects on structure of removing the proteins from their natural membrane environment. As an alternative, it has recently been demonstrated that membrane proteins can be effectively isolated, in the absence of detergents, using a styrene maleic acid co-polymer (SMA). This approach yields SMA lipid particles (SMALPs) in which the membrane proteins are surrounded by a small disk of lipid bilayer encircled by polymer. Here we use the Escherichia coli secondary transporter AcrB as a model membrane protein to demonstrate how a SMALP scaffold can be used to visualise membrane proteins, embedded in a near-native lipid environment, by negative stain electron microscopy, yielding structures at a modest resolution in a short (days) timeframe. Moreover, we show that AcrB within a SMALP scaffold is significantly more active than the equivalent DDM stabilised form. The advantages of SMALP scaffolds within electron microscopy are discussed and we conclude that they may prove to be an important tool in studying membrane protein structure and function
Membrane Transport Proteins: The Proton-Dependentë±Oligopeptide Transporter Family
No full textClinical governance : the next hype?
Full text linkClinical governance is to be the main vehicle for continuously improving the quality of patient care and developing the capacity to maintain high standards. The World Health Organisation has divided the approach into four principle aspects: professional performance; resource use; risk management; patient involvement. In this article the author seeks to explain the terms and their contribution to the whole scenario of Clinical governance. An overview of the local scene in the light of these concepts is also discussed.peer-reviewe
Single- and Multi-carrier Quadrature Amplitude Modulation: Principles and Applications for Personal Communications, WATM and Broadcasting: 2nd
No full textSingle- and Multi-carrier Quadrature Amplitude Modulation Principles and Applications for Personal Communications, WLANs and Broadcasting L. Hanzo Department of Electronics and Computer Science, University of Southampton, UK W. Webb Motorola, Arlington Heights, USA formerly at Multiple Access Communications Ltd, Southampton, UK T. Keller Ubinetics, Cambridge Technology Centre, Melbourn, UK formerly at Department of Electronics and Computer Science, University of Southampton, UK Motivated by the rapid evolution of wireless communication systems, this expanded second edition provides an overview of most major single- and multi-carrier Quadrature Amplitude Modulation (QAM) techniques commencing with simple QAM schemes for the uninitiated through to complex, rapidly-evolving areas, such as arrangements for wide-band mobile channels. Targeted at the more advanced reader, the multi-carrier modulation based second half of the book presents a research-orientated outlook using a variety of novel QAM-based arrangements. * Features six new chapters dealing with the complexities of multi-carrier modulation which has found applications ranging from Wireless Local Area Networks (WLAN) to Digital Video Broadcasting (DVB) * Provides a rudimentary introduction for readers requiring a background in the field of modulation and radio wave propagation * Discusses classic QAM transmission issues relevant to Gaussian channels * Examines QAM-based transmissions over mobile radio channels * Incorporates QAM-related orthogonal techniques, considers the spectral efficiency of QAM in cellular frequency re-use structures and presents a QAM-based speech communications system design study * Introduces Orthogonal Frequency Division Multiplexing (OFDM) over both Gaussian and wideband fading channels By providing an all-encompassing self-contained treatment of single- and multi- carrier QAM based communications, a wide range of readers including senior undergraduate and postgraduate students, practising engineers and researchers alike will all find the coverage of this book attractive
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