1,721,421 research outputs found

    The geographic origin of old Italian cypresses in North Italy revealed by nuclear SSR markers

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    The Italian cypress (Cupressus sempervirens L.) has an important role in the characterization of Mediterranean landscape mainly for its aesthetic and ornamental function and also because it is an excellent pioneer specie for reforestation and for timber production. Since antiquity cypress has been cultivated far beyond its natural geographic range for religious and aesthetic reason. At present its geographic distribution is characterized by disjoint populations or single plants. In this study genetic variability of 600 old cypress trees belonging to 26 populations from different part of North and Central Italy and one population from Turkey has been studied using SSR markers. Nine polymorphic SSR primers couples producing a total of 104 alleles. Turkey showed 15 private alleles. The number of observed alleles in all the populations per locus varied from 8 to 22. Values of Nei’ s gene diversity and Shannon information index suggesting that populations are characterized by low genetic variability probably due to the strong anthropic pressure that this species has undergone. AMOVA revealed a very strong differentiation between Turkey and all Italian populations and a good significant differentiation among Italian groups. UPGMA dendrogram shows two main clusters, one with all the North-Estern Italian populations and the second one with Tuscanians and North-Western Italian populations. The only exception is the population from Bozen. These results suggest that the artificial transfer of cypress reproductive materials highlighting the human-mediated manipulation of cypress populations in Italy and the political and commercial barriers that were present before the first world war

    Diabetic retinopathy: are we really doing all we can for our patients?

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    Although if is now possible to significantly reduce the incidence of blindness secondary to diabetes mellitus, large numbers of diabetic patients still experience visual loss due to retinal complications of the disease. This implies that protocols for the diagnosis and treatment of diabetic retinopathy used in the various multicenter trials have not yet been transferred into routine clinical practice. In countries where ocular complications of diabetes have been managed on the basis of well-codified protocols for several years, the incidence of visual loss among diabetic patients has been significantly reduced. It is absolutely essential to introduce screening and treatment protocols for diabetic retinopathy promptly everywhere. Their application is within the reach of every ophthalmologist. Patients must be informed that it is important to have regular full ophthalmological examinations, before any symp toms occur; the guidelines for screening and management of retinopathy must be known and accepted by the medical community. Spreading this information among primary care physicians, ophthalmologists and diabetologists is a fundamental step in improving the health care of the diabetic patient

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Molecular approaches for low-cost point-of-care pathogen detection in agriculture and forestry

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    Early detection of plant diseases is a crucial factor to prevent or limit the spread of a rising infection that could cause significant economic loss. Detection test on plant diseases in laboratory can be laborious, time consuming, expensive and normally requires specific technical expertise. Moreover in the developing Countries it is often difficult to find laboratories equipped for this kind of analysis. Therefore, in the last years a high effort has been made for the development of fast, specific, sensitive and cost-effective tests that can be successfully used in plant pathology directly in the field, by low-specialized personnel using minimal equipment. Nucleic acid-based methods have proven to be a good choice for the development of detection tools in several fields, such as human/animal health, food safety and water analysis and their application in plant pathogen detection is becoming more and more common. In the present review, the more recent nucleic acid-based protocols for point-of care plant pathogen detection and identification are described and analyzed. All these methods have a high potential for early detection of destructive diseases in agriculture and forestry; they should help making molecular detection for plant pathogens accessible to anyone, anywhere and at anytime. We do not suggest that on site methods should replace completely lab-testing, which remains crucial for more complex researches, such as identification and classification of new pathogens or the study of plant defence mechanisms. Instead, POC analysis can provide a useful, fast and efficient preliminary in field screening that is crucial in the struggle against plant pathogens
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