1,720,983 research outputs found
Life VAIA: agroforestazione con mirtillo selvatico per il recupero dei territori alpini danneggiati
No full textPotatura su misura: verso una gestione agronomica personalizzata delle nuove varietà di mirtillo
No full textRipening behavior in red flesh ‘Kissabel®’ apple fruit during postharvest
No full textIn the last few years, the cultivation of red flesh apple cultivars received great attention due to their novelty and valuable nutraceutical properties, considering their higher anthocyanin and phenols content than white flesh apple cultivars. Nevertheless, red flesh cultivars can be characterized by a low storability, being therefore prone to develop internal browning disorder. In this preliminary work, we investigated the postharvest ripening behavior of the red flesh apple R201-‘Kissabel®’, a late season cultivar, in comparison with the white pulp apple ‘Golden Delicious’, generally considered as the standard reference cultivar for apple. For this purpose, fruit were collected at commercial harvest and kept for one month at room temperature in regular atmosphere. The cortex of five apple was sampled every two days along this period. Total RNA was extracted, reverse transcribed and RT-qPCR were performed on specific ripening related genes, such as ethylene biosynthetic genes (MdACS3, MdACS1 and MdACO1) and the polygalacturonase-1 (MdPG1). Moreover, proton-transferreaction mass spectrometry (PTR-ToF-MS) was employed to quantify ethylene evolution during the stored period. In ‘Kissabel®’, ethylene started to be accumulated immediately after harvest, and one week earlier than ‘Golden Delicious’. Moreover, both cultivars showed a first peak at 9 and 16 days after harvest (DAH) and a second one at 14 and 21 DHA, respectively. Based on the expression profile of selected genes, the first peak coincided with the ripening onset. In ‘Golden Delicious’, indeed, the highest expression level, for all genes, corresponded with the maximum of ethylene production at 16 DAH. In ‘Kissabel®’ the maximum expression was instead detected two days earlier respect the ethylene burst, while the expression pattern of MdACS3 decreased immediately after harvest in both cultivars, as expected. These results represent the first step to better characterize the ripening behavior and storability of this new apple cultiva
Molecular and functional characterization of endophytic bacteria that colonize Vitis vinifera L cv Glera
No full textCandidate genes transcriptional study in ‘Granny Smith’ apples in response to prolonged storage at different conservation strategies
No full textApple (Malus domestica) is one of the most cultivated fruit crop word wide (86.442.716 tons, FAOSTAT 2020) characterized by a mid-late summer harvesting. To satisfy the continue demand of fresh product ensuring a constant availability of this produce to the market, fruit must be stored for prolonged period. Nowadays this is facilitated by the employment of modern storage technologies based on low temperatures (1-4°C), slowing down the entire metabolisms, and the inhibition of the ripening process. The control of the ripening syndrome can be achieved through a controlled atmosphere characterized by a low-oxygen and high CO2 content, inhibiting the biosynthesis of the hormone ethylene, and/or treatment with the ethylene competitor 1-MCP that acting at the perception level efficiently block the signal transduction pathway. With the aims to improve and optimized the storage strategy reducing the quality loss and avoiding and/or limiting the onset of related physiopathology or post-harvest disorder, a deeply understanding of how these conditions affect the ripening process and the quality of apples becomes essential. In this study, different batches of ‘Granny Smith’ apple fruit were stored in various storage conditions, such as: Dynamic-Controlled-Atmosphere (DCA), static Ultra-Low-Oxygen mode (ULO) and classical Regular-Atmosphere, with and without application of 1-MCP treatment. After six months of storage, apples were transferred at room temperature for 12 hours or 7 days simulating a commercial shelf-life. The postharvest ripening physiology was assessed by analyzing the transcriptional pattern of candidate genes involved in ethylene biosynthesis and ripening (ACO1, ACS1 and PG1), signal transduction pathway in response to low-oxygen (ERF-VIIs), sugars and anaerobic metabolisms (amylase, glucose-6-P-isomerase, pyruvate dehydrogenase and alcohol dehydrogenase). The transcriptional profiles of these elements provided important results about the physiological behavior of the fruit stored at different atmospheric conditions
Resilienza al cambiamento climatico: risposta fisiologica e qualitativa di nuove varietà di mirtillo allo stress idrico
No full text
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Candidate gene transcriptional signature unravels the reprogramming occurring in the peel of apple fruit of ‘Granny smith’ during postharvest storage
Full text linkAfter harvest fruit are stored to preserve the quality features established during the on-tree development and maturation, ensuring thus a continuous availability of fresh fruit on the market. For certain fruit species like apple, storage can last for almost a year, especially when coupled with several strategies, such as the reduction of the oxygen concentration or the application of ethylene competitor molecules, like 1-methylcyclopropene (1-MCP). To guarantee the maintenance of the highest quality, the monitoring of the physiological processes ongoing during the postharvest ripening is compelling. For this purpose, 16 genes belonging to key fruit ripening pathways, such as the ethylene and the sugar/fermentation metabolism, have been chosen as potential markers for the molecular characterization of the major changes occurring in the fruit during storage. Among these genes, ACS, PPO, PG1, RAP2-like, and ADH exhibited the most significant differential expression across the various samples. Based on the transcriptional pattern, this set of genes constitutes a valuable molecular tool for a precise and reliable RNA-based monitoring of the postharvest ripening progression and fermentation process in apples. PPO, together with S6PDH, were furthermore employed to inspect the onset of the superficial scald in apple and resulted to correlate with the evaluation of the incidence of this disorder and the accumulation of the sugar alcohol sorbitol, known to play important protecting roles to chilling injuries. The assessment of the transcriptional signature of these elements can facilitate the development of gene expression markers suitable for a more informed investigation of the physiological progression of the postharvest ripening in apples, ultimately leading to the promotion of high-quality stored apples, extending storage time while minimizing postharvest disorders and fruit los
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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