101,945 research outputs found
30. Fr. di cratere a calice a figure rosse apulo con poppa di nave
No full textscheda del frammento di cratere con scena mitologica e poppa naval
Sawmill operation at Poppa, Wayne County, W.Va., Oct. 1900
No full textSawmill operation at Poppa, Wayne County, W.Va., Oct. 1900, b&w mounted on cardboard back reads: Saw mill owner H. W. Walker on horse Sawing timber for GV RR (Guyan Valley) up Guyandotte River, 1900-1901 In picture left to right: Ramon Dial on ox wagon stock lumber on horse H. W. Walker owner & manager Two men on wagon: 2 Ferguson men W. G. Hensley, Teamster David Wroten, slab former Zona Riggs, log roller Alpha Childers, log roller George Ferguson, log roller Leslie W. Self, with saw measured lumber Daddy (Paddy?) Crossen, sawyer John Crossen, from Kenova, W.Va. sawyer at mill Lee Aliff, gin hand Tobe Riggs, gin hand Roll Chadwick, ox team driver, 4 yolk of oxten Poppa, W.Va. on waters of Beach Fork Creek, Wayne Co. Timber 9 x 16 and 12 x 12 28 ft long used on trestles at Tylers Creek, Lovell Creek, below Salt Rock, W.Va.https://mds.marshall.edu/leslie_self_self_papers/1001/thumbnail.jp
Clinical potential of Extracellular Vesicles in Regenerative and Aesthetic Medicine
No full textExtracellular vesicles (EVs) represent a heterogeneous class of spherical particles released by cells that are known for the essential role they play in cell to cell communication in various processes, both physiological and pathological. Over the years, they have been considered an useful tool for the diagnosis and treatment of various diseases, mainly cancer. Lately, however, their use has also extended to other fields of medicine, since they could be administered through minimally or non-invasive approaches in clinical treatments. Among those novel application fields, we find the aesthetic medicine, where the use of EVs aims to support skin rejuvenation and to improve and correct skin-related cosmetic defects, including wrinkles, hair loss, and acne scars. This review provides a general perspective and the latest findings supporting the efficacy of EVs application in Aesthetic Medicine
Applicazione delle anulazioni di cemento per la determinazione dell'età di resti umani
No full textScopo di tale lavoro è verificare l'applicabilità, l'accuratezza e la precisione di tre differenti metodi per la valutazione dell'età biologica: il conteggio delle anulazioni del cemento radicolare, il metodo di Lamendin e quello di Gustafson-Johanson. Per il metodo delle anulazioni del cemento sono stati utilizzati 194 denti decalcificati e non, colorati istologicamente mediante Ematossilina-Eosina, von Kossa e Tricromica di Masson; tale metodo risulta essere quello con il maggior coefficiente di correlazione ed ha inoltre restituito una maggiore precisione, mentre a suo sfavore gioca un tempo di preparazione dei campioni piuttosto lungo. Il metodo è stato testato anche su materile di natura archeologica. Sia il metodo di Lamendin che il Gustafson-Johanson, tendono a sovrastimare l'età entro i 40 anni ed a sottostimarla al di sopra dei 40, ed entrambi i metodi si dimostrano più accurati in un range d'età compreso tra i 35 ed i 45 anni, quello di Gustafson-Johanson è leggermente più preciso, mentre il Lamendin è certamente più semplice da applicare per i non esperti
"Development of a protocol to isolate extracellular vesicles from 3D cultures" in ISEV2022 Abstract Book
No full textIntroduction: Even if the 3D cultures reflect the tumor’s architecture more realistically, there are currently no standardized protocols to isolate extracellular vesicles (EVs) from tumor cell lines cultured in 3D models. Thus, the goal of our study was to develop a simple, practical, reproducible, and economical protocol to isolate EVs from 3D cell cultures.
Methods: Human ovarian cancer CABA I cells were cultured in RPMI+5% FBS (40 nm-filtered) by hanging drop to form tumor spheroids (30000 cells/spheroid). Spheroids were moved to and cultured (1mlmedium/10 spheroids) in Petri dishes coated with an anti-adhesion solution, commercially available. The supernatant was collected twice daily for 5 days, centrifuged at 4◦C at 600xg (15 min), 1,500xg (30 min), and 100,000xg (Rotor 70Ti, Quick-Seal Ultra-Clear tubes, kadj 221, brake 9, Optima XPN-110 Beckman Ultracentrifuge) for 90 min. Isolated EVs were resuspended in PBS, quantified by measuring the EV-associated proteins level (by Bradford method), and analyzed by Transmission Electron Microscopy (TEM), Nanosight (NTA), Western Blots. Scanning Electron Microscopy (SEM) imaged EVs’ release from spheroids. Cell viability in spheroids was evaluated by XTT.
Results: CABA I cultured by hanging drop form compact and proliferating spheroids (334% viability at 48h vs 100% attributed to 24h); SEM revealed the release of many EVs from their surface. Indeed, the supernatant of cultured spheroids contained EVs; the TEM morphological analysis showed spherical and intact particles, which for the most part have a size attributable to sEVs (size range 55-120 nm), as confirmed by NTA. The identity of EVs was further confirmed by positivity for specific EV markers (CD63, CD9, CD81). The amount of released EVs was 0,17-0,53 μg/spheroid.
Summary/Conclusion: Taking advantage of an anti-adhesion solution, compatible with EVs release and collection, we have set a simple method to isolate EVs from 3D spheroids in an effective and reproducible way
"Evaluation of extracellular vesicle-associated TGF-β as a biomarker in patients with endometriosis" in Meeting abstracts of the 3° EVIta symposium
No full textEndometriosis is a common chronic gynecological disease characterized by the presence of endometrial-like
tissue growing outside the uterine cavity. In many patients, it causes debilitating painful symptoms, thus
worsening the quality of life and causing recourse to chronic medical therapy and, eventually, surgery. To
date, the diagnostic gold standard remains minimally invasive laparoscopic surgery followed by histological
confirmation, but extensive research aims to identify a non-invasive serological marker useful for diagnosis
or prediction of the severity; in this regard, studies are also focusing on plasma extracellular vesicles (EVs).
This work aims to evaluate whether the EV-associated TGF-β (Transforming Growth Factor-β) can be used
as a diagnostic biomarker for this pathology; TGF-β, indeed, appears to play a key role in the development
of endometriosis.
To this end, 8 endometriosis patients and 8 controls were initially recruited, from whom blood samples
were collected; the plasma EVs were isolated by differential centrifugation and ultracentrifugation. EVs were
characterized by transmission electron microscopy (TEM), Nanoparticle Tracking Analysis (NTA), and
western blot for the common EV-associated marker CD63. EV-associated TGF-β levels were quantified by
ELISA.
The EVs characterization confirmed their identity; the NTA analysis did not highlight any differences either
in terms of size or concentration between patients and controls. Nevertheless, the quantification of EVassociated
TGF-β highlighted a trend towards higher levels in patients than controls.
Although the number of subjects analyzed was limited, these preliminary data suggest that, in endometriosis
patients, EVs could be characterized by a higher TGF-β level than in healthy subjects, encouraging further
studies
Cancer Three-Dimensional Spheroids Mimic In Vivo Tumor Features, Displaying “Inner” Extracellular Vesicles and Vasculogenic Mimicry
No full textThe role of extracellular vesicles (EVs) as mediators of cell-to-cell communication in cancer progression is widely recognized. In vitro studies are routinely performed on 2D culture models, but recent studies suggest that 3D cultures could represent a more valid model. Human ovarian cancer cells CABA I were cultured by the hanging drop method to form tumor spheroids, that were moved to low adhesion supports to observe their morphology by Scanning Electron Microscopy (SEM) and to isolate the EVs. EVs release was verified by SEM and their identity confirmed by morphology (Transmission Electron Microscopy, TEM), size distribution (Nanoparticles Tracking Analysis), and markers (CD63, CD9, TSG-101, Calnexin). CABA I form spheroids with a clinically relevant size, above 400 mu m; they release EVs on their external surface and also trap "inner" EVs. They also produce vasculogenic mimicry-like tubules, that bulge from the spheroid and are composed of a hollow lumen delimited by tumor cells. CABA I can be grown as multicellular spheroids to easily isolate EVs. The presence of features typical of in vivo tumors (inner entrapped EVs and vasculogenic mimicry) suggests their use as faithful experimental models to screen therapeutic drugs targeting these pro-tumorigenic processes
Bibliographie Hilarion G. Petzold 1958 – 2009 mit Anhang als Einführung
No full textDieses Archiv enthält die Gesamtbibliographie der Werke des Autors nebst einiger Texte „Über H. G. Petzold“ im Schlussteil der Bibliographie sowie einen Anhang mit einer Einführung in die Architektur des Werkes in seinem wissenslogischen Aufbau als Ausarbeitung seines „Tree of Science Modells“ (2007).This archive contains the complete bibliography of the author and some texts about H. G. Petzold, moreover an epilogue with an introduction to the architecture of the works in its epistemological structure and composition and as an elaborations of Petzold’s „Tree of Science Modell (2007).https://www.fpi-publikation.de/polyloge/01-2009-petzold-h-g-gesamtbibliographie-h-g-petzold-1958-2009-updating-november2009/peerReviewedpublishedVersio
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Overview of Geosciences Applied to Forensic Taphonomy: The Interdisciplinary Approach of the Italian Non-Human Research Facility (Ticino-LEAFs)
No full textThe recent surge in the application of geosciences in forensics has yielded significant insights into taphonomy and decomposition processes, particularly with regard to the identification and interpretation of evidence pertaining to the interaction between the cadaver and the outdoor post-depositional environment. Given that a substantial proportion of current knowledge about decomposition dynamics can be attributed to forensic taphonomy experiments utilizing pigs as human models, a literature review was conducted, focusing on experiments incorporating geosciences into their investigative techniques. The results demonstrated the crucial role of this approach in ensuring accurate interpretation of data, both in the context of forensic cases and in experimental research. Furthermore, this manuscript presents the opening of the first Italian non-human forensic taphonomy facility, which was inaugurated by the LABANOF of the University of Milan in 2009 at the Lombardy Park of the Ticino (PV). The facility strives to integrate the use of non-human animals as human models while upholding a high ethical standard and minimizing the construction of artifacts, thereby striving for forensic realism. Furthermore, the most recent project conducted at the Italian FTF establishes a connection between the outcomes of preceding projects and underscores the significance of a pragmatic and intellectual disposition that embraces interdisciplinary collaboration
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