109 research outputs found
Plaza de Toros de Madrid [Material gráfico] : el domingo 12 de junio de 1921 se verificará, si el tiempo no lo impide, una corrida de toros extraordinaria
No full text"Se lidiarán seis toros ... de la acreditada ganadería de Don Andrés Sánchez y Sánchez de Buenabarba (Salamanca), lidiadores, picadores, Eladio Abia, Agustín Ibáñez (Marinero), Pedro Belmonte (Zurito chico); Juan de la Rosa (Azuquita), Fermín Ortega (Cantares); Telesforo González (Anguila) y Maximino Rejón (Cuatro dedos) ... espadas José Roger (Valencia), Juan Luis de la Rosa y Emilio Méndez, banderilleros, Domingo Pons (Chatillo de Valencia), Manuel Lavín y José Paradas; Nicanor Fraga Carranza, José Soto (Sotito) y Francisco Aguilar (Rerre); Ignacio Donoso (Pelucho), Antonio Ferrer (Pastoret II) y José Román Manfredi; José Rufá (Rufaíto) y Antonio Pérez"Incluye los precios de las localidade
Detail-oriented cognitive style and social communicative deficits, within and beyond the autism spectrum: independent traits that grow into developmental interdependence
No full textAt the heart of debates over underlying causes of autism is the "Kanner hypothesis" that autistic deficits in social reciprocity, and a cognitive/perceptual 'style' favouring detail-oriented cognition, co-vary in autistic individuals. A separate line of work indicates these two domains are normally distributed throughout the population, with autism representing an extremity. This realisation brings the Kanner debate into the realm of normative co-variation, providing more ways to test the hypothesis, and insights into typical development; for instance, in the context of normative functioning, the Kanner hypothesis implies social costs to spatial/numerical prowess
(La función del ciclo celular en la auto-renovación y la pluripotencia de las células madre embrionarias humanas)
No full text[eng] Embryonic stem cells (ESC) are derived from the inner cell mass (ICM) of the blastocyst and have the capacity for unlimited proliferation while retaining their potential to differentiate into a wide variety of cell types when cultured in vitro. These properties have made of human embryonic stem cells (hESC) an excellent model on which to study the conditions required for differentiation into specific cell lineages, and consequently the possibility of transplanting specific cell types into damaged tissues.
The continued turn over of ESC while maintaining an undifferentiated state is dependent on unusual cell cycle properties. These unusual proliferative properties are responsible for the generation of tumours when these cells are injected into adult animals. Thus, the study of the unusual proliferative properties of hESC needs to be addressed if their potential is to be realized. To date, most studies of the cell cycle in hESC have been descriptive, lacking functional studies that reveal the mechanisms of how the cell cycle maintains pluripotency and self- renewal of hESC.
In this thesis we sought to understand the mechanisms of cell cycle control of hESC. We asked the question if a single cell cycle gene could regulate the self-renewal or pluripotency properties of hESC using a gain and loss of gene function strategy. We have identified that the protein expression of the p27Kip1 cell cycle inhibitor was low in human pluripotent cells, but its expression increased during differentiation together with changes in the cell cycle structure of pluripotent cells. By adopting a gain and loss of function strategy we increased or reduced its expression in undifferentiating conditions to define its functional role in self-renewal and pluripotency of Hesc, using undifferentiation conditions, overexpression of p27Kip1 in hESC lead to a G1 phase arrest with an enlarged and flattened hESC morphology and consequently loss of self-renewal ability. Loss of p27Kip1 caused an increase of self-renewal while maintaining an undifferentiated phenotype. Moreover, we have shown that a change in the balance of p27Kip1 levels in undifferentiated hESC affects expression of the mesoderm markers: BRACHYURY and TWIST. We have found that expression changes of TWIST are associated with the presence of p27Kip1 protein in the TWIST1 gene promoter.
The results presented in this thesis have interesting implications in stem cell biology. Firstly, these results define that the maintenance of p27Kip1 protein levels at a certain level is essential for self-renewal and pluripotency of hESC. Secondly, p27Kip1 is involved in the regulation of TWIST which is upregulated in several types of tumours and induces an epithelial-mesenchymal transition to facilitate tumor metastasis.[spa] Las células madre embrionarias humanas (conocidas como hESC por sus siglas en inglés de human embryonic stem cells) son derivadas de la masa celular interna de los blastocistos y poseen la capacidad para auto-renovarse ilimitadamente, reteniendo su potencial para diferenciarse hace una amplia variedad de tipos celulares (pluripotencia), cuando son cultivadas in vitro. Estas propiedades permiten el estudio de las condiciones requeridas para la diferenciación hacia linajes específicos y la posibilidad de trasplantar tipos celulares específicos en tejidos dañados.
El continuo recambio de las hESC al mismo tiempo que mantienen un estado de indiferenciación es dependiente de sus inusuales propiedades proliferativas. El objetivo de esta tesis doctoral fue el estudio de los mecanismos de control del ciclo celular de las hESC. Nos preguntamos si una única proteína del ciclo celular podría regular las propiedades de auto-renovación o pluripotencia de las hESC. En esta tesis doctoral identificamos que la expresión proteica del inhibidor del ciclo celular p27Kip1 era baja en diversas líneas celulares humanas pluripotentes pero aumentó durante la diferenciación, al mismo tiempo que la estructura del ciclo celular cambió. Mediante una estrategia de ganancia y pérdida de función, aumentamos o reducimos la expresión de p27Kip1 a fin de definir su función en la auto-renovación y la pluripotencia de las hESC. En condiciones de indiferenciación, la sobreexpresión de p27Kip1 en las hESC resultó en un arresto del ciclo celular en fase G1 y un cambio hacia una morfología más grande y aplanada, y consiguiente pérdida de la propiedad de auto-renovación. La pérdida de p27Kip1 causó un aumento de la auto-renovación manteniendo un fenotipo indiferenciado.
También, hemos demostrado que un cambio en la expresión de p27Kip1 en hESC indiferenciadas afecta la expresión de los reguladores de mesodermo: BRACHYURY y TWIST. Además, hemos descubierto que los cambios en la expresión de TWIST están asociados con la presencia de la proteína p27Kip1 en el promotor de TWIST1.
Estos resultados definen que los niveles de expresión de p27Kip1 son críticos para la auto-renovación y la pluripotencia de las hESC y sugieren una función para p27Kip1 en el control de la transición de epitelio a mesénquima
Impact of tungsten oxidation conditions on the performance of Al2O3/WOx-based CBRAM devices
No full textIn this paper, we assess the impact of different W oxidation conditions on the electrical performance of Cu/AIl(2)O(3)/WON-based CBRAM devices. While HR-TEM characterization carried out on samples with three different oxidation conditions reveals that WON is always in the crystalline phase in our oxidation temperature range, higher oxidation temperature leads to denser and thicker oxides. By performing DC and AC electrical characterization, we demonstrate that a careful engineering of the W oxidation conditions enables to boost the performance of these devices. In particular, we prove that a trade-off between density and thickness must be pursued in order to enlarge the memory window and extend the endurance lifetime with short (10 ns) pulses. (C) 2017 Published by Elsevier B.V.China Scholarship Council [201506010181]SCI(E)ARTICLE56-5917
Corpovoz: ARThivISMO dissidente como estratégia de (r)existência em dança
No full textThis research is based on a theoretical construction that resulted in critical and analytical reflections about artivism Colling (2019), Troi (2018) dance and its political function. In this research, the general purpose is to articulate a discourse on dance, politics and how BODYVOICES (corpovozes) are treated as subversive bodies, in which transgress established normative social stereotypes and still survive. These bodies are living on different surviving instances, and they drive dance manifestations on the interfaces of politics, culture and society. These are some of the proposals and paths to delimitate this research epistemic space with the goal of understanding the BODYVOICES as drivers of subjectivation processes and articulators of their (re)existence discourses as a performative action. Art and life are inseparable in a BODYVOICE, because of subordination processes, effects of dissident social categorization and ills presented in performative actions. In this research, there are elucidations on how political, social and cultural processes are responsible for the condition of abject body, in which Dance takes the task of enunciating these artivisms build from a do-say Setenta (2008) of corposvozes. This research has a theoretical background supported by authors who problematize actions of social paradigms and its effects on attitudinal modifications of dissident bodies of sex, sexuality, gender, class, race and those against the norm in culture-society across history. To emphasize the historical (re)existence of artists bodies in the hiv/aids movement, problematizing and resisting the epidemics of aids discourse Bessa (1997), going beyond hiv virus proposing dissidents works and debating the intersection of art/dance/performance. We seek to understand the political agency that causes tensions in the lives and subjection processes resulted from these bodies, also their resistance discourse taken from semistructured conducted interviews. In this research there is also the artistic/academic trajectory of the author questions about Corpovoz under HIV perspective. The author writes experiences as scribbled narratives (Evaristo,2007) that converse with theoretical assumptions as part of artivism in the arts and dissident action of sexuality in dance.Esta pesquisa parte de uma construção teórica subsidiada numa reflexão crítica e analítica do artivismo Colling (2019), Troi (2018) na Dança. Tem como propósito gerar uma articulação entre Dança e política para discutir corpovozes como subversivos, que sobrevivem e transgridem padrões normativos já estabilizados. Corpos que habitam diferentes instâncias de sobrevivência gerando nas interfaces entre política, cultura e sociedade, manifestos em Dança. Essas são algumas propostas e caminhos que delimitam o espaço epistemológico dessa pesquisa, que propõe o entendimento de Corpovoz como propositor e articulador dos seus discursos de (r)existência como ação performativa. O Corpovoz, em sua ação de proferir a indissociabilidade entre vida e arte, Dança e artivismo expõe estratégias de sustentabilidade, as vezes mínimas, ao mesmo tempo em que dá visibilidade ao seu modo de sobrevivência. Arte e vida no Corpovoz são inseparáveis, pois em sua ação performativa exibe as mazelas e os efeitos da categorização das dissidências e seus processos de subalternização. Elucida que os processos políticos, sociais culturais são responsáveis pela condição de corpo abjeto, no qual a Dança se incube de enunciar os artivismos construídos a partir de um fazer-dizer (Setenta, 2008) dos corposvozes. A pesquisa, dispõe de arcabouço teórico amparado nos autores que problematizam as ações e os efeitos dos paradigmas sociais para a modificação atitudinal em relação aos corpos dissidentes de sexo, sexualidade, gênero, classe e raça contra a norma constituída na cultura-sociedade ao longo da história. A fim de enfatizar a (r)existência histórica dos corpos artísticos que através do movimento hiv/aid para além do vírus hiv, aponta-se trabalhos dissidentes que discutem a intersecção da arte/dança/performance. Para tanto, buscar-se-á compreender os agenciamentos políticos que tencionam a vida e os processos de assujeitamento desses corpos, seus discursos de resistências a partir de entrevistas semiestruturadas. Nessa pesquisa, há, também, minha trajetória artística/acadêmica, vivendo com hiv, implicado nas questões levantadas na perspectiva do Corpovoz, ao trazer no último capítulo minhas experiências como narrativas escrevivenciadas (Evaristo,2007) dialogando com pressupostos teóricos como parte do artivismo no campo das artes dissidentes de sexualidade, classe e cor na dança
The power law relation of spiral waves in the Belousov-Zhabotinsky reaction
No full textThe relationship of the period T-s and the wavelength lambda (s) of spiral waves with the control parameters is systematically studied with the Belousov-Zhabotinksy (BZ) reaction in a spatially extended quasi-two-dimensional system. Our experiments indicate that T-s and lambda (s) not only rely on the concentrations of sulfuric acid and sodium bromate, as indicated in the previous work [A. L. Belmonte, Q. Ouyang, and J. M. Flesselles, J. Phys. II 7, 1425 (1997)] but also have strong relation with the concentration of malonic acid (MA). With the influence of the concentration of MA taken into consideration, a revised power law of spiral waves is suggested, which is qualitatively in agreement with early works of numerical simulations and theoretical analysis. (C) 2000 American Institute of Physics. [S0021- 9606(00)70248-0].Physics, Atomic, Molecular & ChemicalSCI(E)EI1ARTICLE2411280-1128311
Formation of 8-RS-BODIPYs via direct Substitution of 8-MeS-BODIPY by RSH; R = Et, Pr, Bu, tBu, n-C12H25, C6H5, p-MeC6H4, p-MeOC6H4, and 2,6-Me2C6H3
No full textReactions between 8-RS-BODIPY (R = Me, 1) and alkyl and aryl thiols were readily accomplished in dichloromethane (DCM) to provide a synthetic pathway to a range of new 8-organothio-BODIPYs in good yield. The new alkyl 8-RS-BODIPYs, R = Et, 2; Pr, 3; Bu, 4; tBu, 5, n-Dodecyl, 6; exhibit absorption and emission properties essentially unchanged from those of 1 whereas the arylthio analogs, R = Ph, 7; 2,6-Me2C6H3, 8; p-MeC6H4, 9; p-MeOC6H4, 10, exhibit no fluorescence with the exception of 7, and then only in hexane. In common with other related 8-substituted BODIPYs the new 8-alkylthio-BODIPY dyes show decreasing fluorescence intensity as solvent polarity increases. Compounds 2, 3, 7, and 8 were characterized via single crystal X-ray analysis; the alkyl derivatives 2 and 3 exhibited planar BODIPY cores with co-planar organothio- substituents, whereas the aryl derivatives exhibited both BODIPY core deformation and significant twisting about the S-C(8) bond removing co-planarity between the aryl group and the distorted BODIPY core. These deformations coincide with the significantly reduced emission properties.The accepted manuscript in pdf format is listed with the files at the bottom of this page. The presentation of the authors' names and (or) special characters in the title of the manuscript may differ slightly between what is listed on this page and what is listed in the pdf file of the accepted manuscript; that in the pdf file of the accepted manuscript is what was submitted by the author
Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis
No full textWe thank the BBSRC, SULSA BioSKAPE and Pfizer Inc. for funding for a studentship for F.M.R. and the Wellcome Trust (086827, 075470, 099215, 099197 and 101873) and a Wellcome Trust ISSF award (105625), MRC CiC (MC_PC_14114) and MRC Centre for Medical Mycology and University of Aberdeen for funding and a Wellcome Trust Strategic Award (097377) and a Wellcome Trust grant 099197MA to T.F. and FCT Investigator IF/00033/2012 and PTDC/QUI-QUI/112537/2009 to A.S.P. We thank Ian Broadbent, Angus McDonald and Ron Gladue for constructive discussions; Chris Boston and Amanda Fitzgerald for advice on antibody expression and purification; Ed Lavallie and Wayne Stochaj for design and expression of the recombinant Hyr1; Louise Walker for high-pressure freezing of samples for TEM analysis; Jeanette Wagener for endotoxin testing of mAbs for in vivo experiments; Yan Liu of the Glycosciences laboratory for insight in the analysis with N-glycan array; Rebecca Hall and Mark Gresnigt for providing fungal strains; Andrew Limper and Theodore J. Kottom for providing Pneumocystis infected lung tissue extracts; David Williams for C. albicans mannoprotein; Christopher Thornton for A. fumigatus mannoprotein; Katie J. Doores for mAb PGT 128; and Gordon Brown for the murine Fc-Dectin-1. We are grateful to Lucinda Wight, Debbie Wilkinson and Kevin MacKenzie in the Microscopy and Histology Core Facility (Aberdeen University) and Raif Yuecel in the Iain Fraser Cytometry Centre (Aberdeen University) for their expert help with microscopy and cytometry experiments. We are also grateful to the staff at the University of Aberdeen Medical Research Facility for assistance with in vivo experiments and members of the Glycosciences Laboratory for their support of the Carbohydrate Microarray Facility. 18 January 2019 - Author Correction: Single human B cell-derived monoclonal anti-Candida antibodies enhance phagocytosis and protect against disseminated candidiasis F. M. Rudkin, I. Raziunaite, H. Workman, S. Essono, R. Belmonte, D. M. MacCallum, E. M. Johnson, L. Silva, A. S. Palma, T. Feizi, A. Jensen, L. P. Erwig & N. A. R. Gow Nature Communicationsvolume 10, Article number: 394 (2019)Peer reviewe
Polymer-based treatments to control runoff, leachate and erosion from engineered slopes at Simfer Mine, Guinea, Africa
No full textIt is necessary to understand the erodibility and hydrological response of mine-site slope forming materials (SFMs), because of increasing awareness of the environmental impacts of mining. Steep engineered slopes in high intensity rainfall environments present a serious erosion risk. Temporary surface stabilisers, such as polyacrylamides (PAMs) and polyvinylacrylic latex (PVALs) are potentially cost effective erosion control solutions. In this study PAM and PVAL efficacy to reduce runoff, leachate and erosion was assessed at two application rates, with and without gypsum on SFMs from an iron ore mine in Guinea (West Africa). NSPASS (near-surface photogrammetry assessment of slope forming materials’ surface roughness) is a novel method that integrates digital image capture and GIS. It is shown to detect and quantify surface micro-relief changes of 2-3 mm, not visible to the naked eye.
As expected, soil and non-soil SFMs were significantly different in terms of their physical and chemical properties. Phase I of the study investigated the erodibility of ten SFMs, including soil, ore and waste-rock. The results indicate that the hydrological response to rainfall of most SFMs is to generate leachate. Weathered phyllite (PHY-WEA) is the most erodible SFM by both runoff and leachate. Multiple regression analysis demonstrated that magnetic susceptibility, mineralogy and dry aggregate distribution; parameters not commonly assessed in erosion studies, are important in explaining SFM erodibility and hydrological response.
Phase II evaluated critically the effectiveness of three commercially available polymer solutions (two PAMs and one PVAL) at reducing runoff, leachate and erosion from four of the most erodible SFMs identified in Phase I. The results indicate that some PAM and PVAL treatments significantly reduce runoff, leachate and erosion. Polymer efficacy is highly dependent on the physical and chemical properties of the SFM, as well as the mechanism of polymer to SFM adsorption. Increasing the application rate of select treatments lowered leachate volumes, runoff and leachate total sediment loads. Contrary to previous studies, gypsum amendments did not significantly improve polymer efficiency.
This research has added to our understanding of the erodibility and hydrological response of soil and non-soil SFMs. This is the first study to evaluate critically the efficacy of PVALs in controlling erosion from mine-site SFMs. Future studies should continue to optimise NSPASS performance in monitoring changes in surface micro-relief
Mitochondrial replacement in human oocytes carrying pathogenic mitochondrial DNA mutations
No full textErratum in
• Author Correction: Mitochondrial replacement in human oocytes carrying pathogenic mitochondrial DNA mutations.
Kang E, Wu J, Gutierrez NM, Koski A, Tippner-Hedges R, Agaronyan K, Platero-Luengo A, Martinez-Redondo P, Ma H, Lee Y, Hayama T, Van Dyken C, Wang X, Luo S, Ahmed R, Li Y, Ji D, Kayali R, Cinnioglu C, Olson S, Jensen J, Battaglia D, Lee D, Wu D, Huang T, Wolf DP, Temiakov D, Belmonte JCI, Amato P, Mitalipov S.Nature. 2019 Mar;567(7747):E5-E9. doi: 10.1038/s41586-019-0876-1.PMID: 30814738Maternally inherited mitochondrial (mt)DNA mutations can cause fatal or severely debilitating syndromes in children1,2,3, with disease severity dependent on the specific gene mutation and the ratio of mutant to wild-type mtDNA (heteroplasmy) in each cell and tissue4. Pathogenic mtDNA mutations are relatively common, with an estimated 778 affected children born each year in the United States5. Mitochondrial replacement therapies or techniques (MRT) circumventing mother–to–child mtDNA disease transmission involve replacement of oocyte maternal mtDNA6,7,8. Here we report MRT outcomes in several families with common mtDNA syndromes. The mother’s oocytes were of normal quality and mutation levels correlated with those in existing children. Efficient replacement of oocyte mutant mtDNA was performed by spindle transfer8, resulting in embryos containing >99% donor mtDNA. Donor mtDNA was stably maintained in embryonic stem cells (ES cells) derived from most embryos. However, some ES cell lines demonstrated gradual loss of donor mtDNA and reversal to the maternal haplotype. In evaluating donor–to–maternal mtDNA interactions, it seems that compatibility relates to mtDNA replication efficiency rather than to mismatch or oxidative phosphorylation dysfunction. We identify a polymorphism within the conserved sequence box II region of the D-loop as a plausible cause of preferential replication of specific mtDNA haplotypes. In addition, some haplotypes confer proliferative and growth advantages to cells. Hence, we propose a matching paradigm for selecting compatible donor mtDNA for MRT
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