169,807 research outputs found
MTTV: an interactive trajectory visualization and analysis tool
No full textWe present an interactive visualizer that enables the exploration, measurement, analysis and manipulation of trajectories. Trajectories can be generated either automatically by multi-target tracking algorithms or manually by human annotators. The visualizer helps understanding the behavior of targets, correcting tracking results and quantifying the performance of tracking algorithms. The input video can be overlaid to compare ideal and estimated target locations. The code of the visualizer (C++ with openFrameworks) is open sourc
HIV-1 p17 binds heparan sulfate proteoglycans to activated CD4(+) T cells
No full textWe have previously shown that HIV-1 p17 binds to activated peripheral blood mononuclear cells and enhances secretion of pro-inflammatory
cytokines, but we were unable to define a ligand on activated cells. In this work we evaluate the hypothesis that HIV-1 p17 may be a heparin/heparan
sulfate-binding protein.
HIV-1 p17 contains C- and N-terminal sequences with positively charged residues and a consensus cluster for heparin binding.We demonstrated
by affinity chromatography that HIV-1 p17 binds strongly to heparin-agarose at physiological pH. Soluble heparins and heparan sulfate but not
chondroitin 4-sulfate and dextran sulfate inhibit binding of HIV-1 p17 to heparin solid phase and to activated CD4+ T cells. Furthermore the
inhibition of cell sulfatation by chlorate treatment completely counteracts HIV-1 p17 binding to activated cells.
These results indicate for the first time that HIV-1 p17 can be ascribed to the heparin binding protein family and suggest that this interaction
might play a key role in the ability of the protein to induce an inflammatory effect on activated cells
Determination of morphine in the hair of heroin addicts by high performance liquid chromatography with fluorimetric detection
No full textA procedure has been presented for the quantitative determination of morphine contained in the hair of heroin addicts, by means of heat-acid hydrolysis, pre-column dansyl derivatization, straight phase liquid chromatography, and fluorescence detection. External standardization was used. Intra-assay and day-to-day variation coefficients were 5.6 and 7.8%, respectively (n = 10), when hair containing 1 ng/mg of morphine was assayed. Hair samples of 22 heroin addicts showed positive results in the range 0.08 to 15.7 ng/mg. No false positive results were found in 20 control subjects. A close correlation was shown between high performance liquid chromatography and radioimmunoassay results (y = 0.97x + 0.26)(r = 0.997, n = 15). Morphine hair content results significantly correlated with the grade of heroin use roughly estimated by means of serial determinations of morphine in urines during the last months before hair sampling
HIV p17 reverses the anti-inflammatory activity of IL-4 on IL-15 stimulated monocytes and modulates their ability to secrete MIP-1 alpha
No full textMonocytes play a central role in the immune system by producing and reacting to different soluble factors. Cytokine dysregulation is an hallmark
in HIV-infected individuals and it is one of the most significant factors leading to impaired immunity in HIV/AIDS disease.
This study investigates the possibility of modulation in the secretion of some inflammatory cytokines and chemokines induced by HIV p17 in
monocytes.
The results show that p17, while ineffective on resting monocytes, exerts an inflammatory action on IL-4 mediated inhibition of TNF-alpha and
IFN- gamma production induced by IL-15 stimulation. In addition, p17 is able to reduce MIP-1alpha secretion, but unable to influence IL-6 production.
The ability of HIV p17 to contribute to an altered pattern of secreted soluble factors might imply a key role for this viral protein in the development
of AIDS pathogenesi
Impiego di una formula adattata a base di aminoacidi in bambini con allergia al latte vaccino
No full textHIV-1 p17 matrix protein interacts with heparan sulfate side chain of CD44v3, syndecan-2, and syndecan-4 proteoglycans expressed on human activated CD4+ T cells affecting tumor necrosis factor alpha and interleukin 2 production.
No full textAllergia alle proteine del latte vaccino: tolleranza di una formula adattata a base di aminoacidi.
No full textInhibitory effects of galanin on growth hormone (GH) release in cultured GH-secreting adenoma cells: comparative study with octreotide, GH-releasing hormone, and thyrotropin-releasing hormone.
No full textCapillary electrophoresis for the investigation of illicit drugs in hair: determination of cocaine and morphine
No full textToxicological analysis of hair is becoming a popular method for investigating past, chronic use of illicit drugs. Several analytical methods using immunometry, chromatography and mass spectrometry have been reported. In this work, capillary electrophoresis was first used for the determination of illicit drugs, such as cocaine and morphine, in the hair of heroin and cocaine users. After rapid washing, hair samples were incubated overnight in 0.25 M HCl at 45 degrees C and the mixtures were extracted with ready-to-use Toxi-tubes A. The organic phase was evaporated and the residue dissolved in a suitable amount of electrophoresis buffer. Free zone capillary electrophoretic determinations of morphine, the main heroin metabolite, and cocaine were accomplished in 0.05 M borate buffer (pH 9.2) at a potential of 15,000 V, with UV detection at 214 and 238 nm, respectively. The use of the less selective wavelength of 200 nm allowed the simultaneous detection of both compounds. Efficient separations (up to 350,000 theoretical plates) and accurate and precise determinations (intra-day R.S.D.s in the range 3-5%) of cocaine and morphine in hair extracts were easily achieved. The analytical sensitivity was sufficient to determinate as little as 0.15 ng/mg of cocaine and morphine in hair using 100-mg samples. Interferences from more than 90 therapeutic drugs and drugs of abuse were excluded
Two-dimensional electrophoresis and western-blotting analyses with anti Ara h 3 basic subunit IgG evidence the cross-reacting polypeptides of Arachis hypogaea, Glycine max, and Lupinus albus seed proteomes
No full textThe allergenicity of seed storage proteins, the major components of edible legume seeds, may cause serious reactions in both children and adult population. Updated methodologies for evaluation of the activity of these proteins are needed. In this paper we used two-dimensional (2D) electrophoretic techniques to investigate the immuno-cross-reactivities of anti Ara h 3 basic subunit IgG to the seed proteomes of three legume species, namely, peanut, soybean, and lupin. The seed proteins, extracted with two different procedures, were separated by 2D electrophoresis, and the electrophoretic maps were analyzed by Western blot. In peanut proteome the antibodies strongly reacted with the 23 kDa polypeptides, corresponding to Ara h 3 basic isoforms, the antigen they were raised to, and three unidentified acidic polypeptides near 45 kDa. Remarkable cross-reactivities with lupin and soybean Ara h 3 homologous polypeptides and nonrelated proteins, namely, lupin conglutin γ and soybean Bg7S, were detected. Therefore, these proteins may be regarded as new putative allergens. The present findings show the potentiality of 2D electrophoresis in the identification of food allergens and open the way to the traceability of the new cross-reacting proteins in the food chain
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