169,906 research outputs found
Azygophleps aburae Plotz 1880
No full textAzygophleps aburae (Plotz, 1880) (Fig. 45) Zeuzera aburae Plotz, 1880: 77. Type locality. Bei Aburi [Ghana]. Type material. location unknown; lost? Distribution. Zimbabwe, Kenya, Ghana, Cameroon, Malawi, Sudan (Schoorl 1990; Yakovlev 2011). Material examined. 2 males, Dzalanyama forest, Lilongwe distr., C. Malawi, 1300 m, 21.04. 2006, leg. R. Murphy (RMM); 2 males, Ntchisi forest, C. Malawi, 1600 m, 10 – 17.05.2002, leg. R. Murphy (RMM); 1 male, C. Malawi, Kasungu N.P., Lifupa Lodge, 13 °05' 12 "S 33 °08' 17 "E, h 1035 m, 22.04. 2011, leg. R. Yakovlev (RYB).Published as part of Yakovlev, Roman V. & Murphy, Raymond J., 2013, The Cossidae (Lepidoptera) of Malawi with descriptions of two new species, pp. 371-393 in Zootaxa 3709 (4) on page 390, DOI: 10.11646/zootaxa.3709.4.5, http://zenodo.org/record/24896
C-terminal fluorescent labeling impairs functionality of DNA mismatch repair proteins
No full textThe human DNA mismatch repair (MMR) process is crucial to maintain the integrity of the genome and requires many different proteins which interact perfectly and coordinated. Germline mutations in MMR genes are responsible for the development of the hereditary form of colorectal cancer called Lynch syndrome. Various mutations mainly in two MMR proteins, MLH1 and MSH2, have been identified so far, whereas 55% are detected within MLH1, the essential component of the heterodimer MutLα (MLH1 and PMS2). Most of those MLH1 variants are pathogenic but the relevance of missense mutations often remains unclear. Many different recombinant systems are applied to filter out disease-associated proteins whereby fluorescent tagged proteins are frequently used. However, dye labeling might have deleterious effects on MutLα's functionality. Therefore, we analyzed the consequences of N- and C-terminal fluorescent labeling on expression level, cellular localization and MMR activity of MutLα. Besides significant influence of GFP- or Red-fusion on protein expression we detected incorrect shuttling of single expressed C-terminal GFP-tagged PMS2 into the nucleus and found that C-terminal dye labeling impaired MMR function of MutLα. In contrast, N-terminal tagged MutLαs retained correct functionality and can be recommended both for the analysis of cellular localization and MMR efficiency
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
N‐terminus of hMLH1 confers interaction of hMutLα and hMutLβ with hMutSα
No full textMismatch repair is a highly conserved system that ensures replication fidelity by repairing mispairs after DNA synthesis. In humans, the two protein heterodimers hMutSα (hMSH2‐hMSH6) and hMutLα (hMLH1‐hPMS2) constitute the centre of the repair reaction. After recognising a DNA replication error, hMutSα recruits hMutLα, which then is thought to transduce the repair signal to the excision machinery. We have expressed an ATPase mutant of hMutLα as well as its individual subunits hMLH1 and hPMS2 and fragments of hMLH1, followed by examination of their interaction properties with hMutSα using a novel interaction assay. We show that, although the interaction requires ATP, hMutLα does not need to hydrolyse this nucleotide to join hMutSα on DNA, suggesting that ATP hydrolysis by hMutLα happens downstream of complex formation. The analysis of the individual subunits of hMutLα demonstrated that the hMutSα–hMutLα interaction is predominantly conferred by hMLH1. Further experiments revealed that only the N‐terminus of hMLH1 confers this interaction. In contrast, only the C‐terminus stabilised and co‐immunoprecipitated hPMS2 when both proteins were co‐expressed in 293T cells, indicating that dimerisation and stabilisation are mediated by the C‐terminal part of hMLH1. We also examined another human homologue of bacterial MutL, hMutLβ (hMLH1–hPMS1). We show that hMutLβ interacts as efficiently with hMutSα as hMutLα, and that it predominantly binds to hMutSα via hMLH1 as well
Mitomycin C in highly myopic eyes - Author reply
No full textOphthalmology. 2005 Feb;112(2):208-18; discussion 219.
Mitomycin C modulation of corneal wound healing after photorefractive keratectomy in highly myopic eyes.
Gambato C, Ghirlando A, Moretto E, Busato F, Midena E.
SourceRefractive Surgery Service and Antimetabolite Therapy Research Unit, Department of Ophthalmology, University of Padova, Padova, Italy.
Abstract
PURPOSE: To evaluate the role of topical mitomycin C in corneal wound healing (CWH) after photorefractive keratectomy (PRK) in highly myopic eyes.
DESIGN: Prospective, double-masked, randomized clinical trial.
PARTICIPANTS: Seventy-two eyes of 36 patients affected by high (>7 diopters) myopia.
METHODS: In each patient, one eye was randomly assigned to PRK with intraoperative topical 0.02% mitomycin C application, and the fellow eye was treated with a placebo. Postoperatively, mitomycin C-treated eyes received artificial tears (3 times daily, tapered in 3 months), whereas the fellow eye was treated with fluorometholone sodium 2% and artificial tears (3 times daily, tapered in 3 months).
MAIN OUTCOME MEASURES: Uncorrected visual acuity (UCVA) and best-corrected visual acuity (BCVA), contrast sensitivity, manifest refraction, and biomicroscopy. Contrast sensitivity was determined using the Pelli-Robson chart. Corneal confocal microscopy documented CWH.
RESULTS: Mean follow-up was 18 months (range, 12-36). No side effects or toxic effects were documented. At 12-month follow-up examination, UCVAs (logarithm of the minimum angle of resolution) were 0.4+/-0.48 and 0.5+/-0.53 (P = .03) in mitomycin C-treated eyes and corticosteroid-treated eyes, respectively. At 1 year, corneal haze developed in 20% of corticosteroid-treated eyes, versus 0% of mitomycin C-treated eyes. At 12, 24, and 36 months, corneal confocal microscopy showed activated keratocytes and extracellular matrix significantly more evident in untreated eyes (Ps = 0.004, 0.024, and 0.046, respectively).
CONCLUSION: Topical intraoperative application of 0.02% mitomycin C can reduce haze formation in highly myopic eyes undergoing PRK.
Comment in
Ophthalmology. 2006 Feb;113(2):357; author reply 357-8
Was ist ein geeigneter Maßstab, um die Ergebnisse von Lokalisationsergebnissen interpretieren zu können?
No full textHintergrund: Das Richtungshören ist eine der Grundfunktionen des binauralen Hörens. Durch einen Vergleich der beidohrigen Signale, kann der Ort eines Hörereignisses bezüglich Richtung und Entfernung bestimmt werden. In verschiedenen Alltagssituationen ist die präzise Ortung von Schallquellen von großer Bedeutung. Neben der Ortung von möglichen Gefahrenquellen, spielt die Lokalisationsfähigkeit auch bei der Kommunikation eine grundlegende Rolle. So ist die Lokalisation eines Sprechers für das Verstehen von Sprache im Störgeräusch entscheidend. Im Vergleich zu Normalhörenden, zeigen Patienten mit Hörstörungen zum Teil erhebliche Defizite beim Richtungshören. Trotzdem ist die Überprüfung der Lokalisationsleistung bisher nicht flächendeckend im klinisch-audiologischen Bereich vertreten. In Forschungslaboren gibt es viele unterschiedliche Messmethoden, die jedoch nicht in der Diagnostik und Rehabilitation eingesetzt werden. Ferner ist auch die Darstellung und Auswertung der Ergebnisse entscheidend für die Begutachtung und Interpretation der jeweiligen Lokalisationsleistungen. Gleichwohl wäre eine Analysemethode wünschenswert, die etwaige Faktoren der unterschiedlichen Messsysteme berücksichtigt und die Berechnung eines geeigneten Indexes zulässt.Material und Methoden: Die Messungen finden an einem modifizierten Mainzer Kindertisch (ERKI-Setup, Plotz & Schmidt ) statt. Im Bereich von ±90° werden die Stimuli (300 ms; 65 dB SPL) in 5°-Schritten über 37 Wiedergabewinkel (5 reale, 32 virtuelle Schallquellen) präsentiert. An der Studie nahmen normalhörende Kinder, sowie normalhörende und schwerhörende Erwachsene teil.Ergebnisse: Für den Tagungsbeitrag werden die Ergebnisse aus den ERKI-Messungen mit verschiedenen Analysemethoden ausgewertet und zueinander in Bezug gesetzt. Ein Ansatz ist, neben dem RMS-Wert bzw. den RMS-Fehler auch den "localization sensitivity index (LSI)" nach der Methode von Zheng et al. zu berechnen. Die Arbeitsgruppe von Zheng kritisierte in ihrer Studie die Aussagekraft des RMS-Wertes bezogen auf die Lokalisationsfähigkeit des Probanden. So erhielten sie z.B. für zwei Probanden sehr ähnliche RMS-Werte, obwohl die Muster der Lokalisationen unterschiedlich waren. Zudem gab es erste erfolgreiche Ansätze, die Ergebnisse der Lokalisationsexperimente über antrainierte Algorithmen auszuwerten (Lückehe et al. ). Der Algorithmus sollte entscheiden, ob es sich bei den ERKI-Ergebnissen um die eines Kindes oder eines Erwachsenen handelte
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
A Multi-Language Comparison of Influences on Author Verification using Character N-Grams
No full textWe create a new multi-language corpus for author verification based on Wikipedia talkpages, and evaluate the influence that differences in topic and time have on character n-gram author profiles. Topic alignment between two texts is found to increase author verification precision, and an authors writing style is found to change over time, but not more significantly after 3 years than after 1 year.Information ArchitectureWISElectrical Engineering, Mathematics and Computer Scienc
A 0.12mm<sup>2</sup> Wien-Bridge Temperature Sensor with 0.1°C (3σ) Inaccuracy from -40°C to 180°C
No full text Resistor-based temperature sensors can achieve much higher resolution and energy efficiency than conventional BJT-based sensors [1], but they typically occupy more area (> 0.25 mm 2 ) and have lower operating temperatures (le 125 {circ} {C}) [2]-[4]. This work describes a 0.12mm 2 resistor-based sensor that uses a Wien-bridge (WB) filter to achieve 0.1 {circ} {C} (3 sigma) inaccuracy from - 40 {circ} {C} to 180 {circ} {C}. Compared to a state-of-the-art WB sensor [4], it occupies 6 × less area and achieves comparable relative accuracy over a 76% wider operating range. Session 10.3 Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public.Electronic InstrumentationMicroelectronic
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