102,046 research outputs found
Chapter 3. PROBABILISTIC TOOLS B. Bagen (Lead Editor), D. Cirio, D. Jacobson, S. Massucco, G. Migliavacca, M. Papic, A. Pitto and S. Sterpu
No full textLa presencia de los estándares del Acuerdo de Escazú sobre acceso a la información, participación y acceso a la justicia ambiental en el derecho comparado europeo
No full textRecensione al volume: Scaffardi L, Formici G (a cura di), Novel Foods and Edible Insects in the European Union: An Interdisciplinary Analysis, Cham, Springer Nature, 2022
No full textCHAPTER 4 THE TRUE NEED FOR PROBABILISTIC AND RISK-BASED APPROACHES FOR PLANNING, AND THE DIRECTION FOR FUTURE WORK R. Boyer, E. Ciapessoni, D. Cirio, B. Gou, S. Guillon, I. Hiskens, S. Massucco, A. Pitto, P. Pourbeik (Lead Editor), W. Sattinger, M. Schläpfer, S. Sterpu and C. Vournas
No full textQuantitative CT-assisted osteodensitometry of femoral adaptive bone remodelling after uncemented total hip arthroplasty
No full textRocco P. Pitto, Aknaksha Bhargava, Salil Pandit, Cameron Walker, Jacob T. Munr
Spatial expression of DNA topoisomerase I genes during cell proliferation in Daucus carota.
No full textThe spatial expression of carrot (Daucus carota L.) top1 genes encoding the two isoforms of the enzyme DNA topoisomerase I (EC 5.99.1.2) was investigated. In situ hybridization analysis performed with a probe recognizing both top1 transcripts provided evidence that in explanted hypocotyls induced to proliferate in vitro by the addition of the growth regulator 2,4-dichlorophenoxyacetic acid (2,4-D), the mRNA accumulation parallels the proliferation of provascular cells of the stelar cylinder. During somatic embryogenesis, the histological distribution of top1 transcripts was strongly evident at the stage of torpedo-shaped embryos, but gene expression was not only restricted to meristematic regions. When the spatial localization was extended to carrot vegetative apices and the investigation was carried out with specific probes for top1alpha and top1beta, both transcripts preferentially accumulated in tissues having mitotic activity
Utilizzo dei fibroblasti in coltura nella diagnostica biochimica delle malattie da accumulo lisosomale
No full textmicroRNA(interference) networks are embedded in the gene regulatory networks
No full textmicroRNAs (miRNAs) are a class of endogenous 22-25 nt single-stranded RNA molecules that regulate gene expression post-transcriptionally. They are highly conserved among species with distinct temporal and spatial patterns of expression, each of them potentially interacting with hundreds of messenger RNAs. Since miRNAs, like transcription factors (TFs), are trans-acting factors that interact with cis-regulatory elements, they potentially generate a complex combinatorial code. Moreover, as TFs and genes containing binding sites for TFs have a high probability of being targeted by miRNAs, the basic interplay miRNA/TF renders miRNAs key components of gene regulatory networks. Several biological processes, including diseases such as cancer, have been causatively associated to disturbances of miRNAs/TF interplay both in vitro and in vivo. These aspects, cumulatively, indicate that miRNAs and transcription factors have a crucial role in determining cellular behaviour, highlighting the role of small RNA molecules in regulatory mechanisms and indicating other routes in the evolutionary path of gene expression
Sialidase in Cerebellar Granule Cells Differentiating in Culture
No full textAbstract: The optimal conditions for the assay of sialidase in cerebellar granule cells cultivated in vitro, established using f3H]GDla and 2′‐(4‐methylumbelliferyl)‐α‐D‐N‐acetyl‐neuraminic acid (MUB‐NeuNAc) as substrates, were the following: pH optimum for both substrates, 3.9; optimal molarity of sodium acetate/acetic acid buffer, 0.05 M with [3HJGDla and 0.1 M for MUB‐NeuNAc; substrate concentration for apparent maximal activity, 0.5 mM for MUB‐NeuNAc and 0.1 mM for [3H]GDla; enzyme activity linear with time up to 30 min with MUB‐NeuNAc and up to 90 min with f3HJGDla; and enzyme activity linear with enzyme protein content up to 80 μg with MUB‐NeuNAc and up to 20 μg with f3H]GDla. The assay with [3H]GDla required the presence of Triton X‐100 in a molar ratio to GDla of 15:1. Poly‐L‐lysine, which was used for plating the cells, was capable of decreasing sialidase activity against [3H]GDla/ Triton X‐100 when added to the incubation mixture. However, it had no effect on the enzyme working on MUB‐NeuNAc. Using no more than 20 μg of cellular protein, the contamination, if any, by poly‐L‐lysine released from the dish was below the concentration limit exhibiting inhibition. Using the above optimal conditions, sialidase activity was measured during cerebellar granule cell differentiation in culture. From day 0 to day 7–8 in culture, the enzyme activity rose from 20 to 130 nmol of product released/h/mg of protein with MUB‐NeuNAc and from 1 to 100 nmol of product released/ h/mg of protein with [3H]GDla. The values of enzyme activity in differentiated granule cells are the highest ever reported for mammalian sialidases in isolated cells or tissue homogenates. In fully differentiated cells, the sialidase activity against endogenous substrates was 4.2 nmol of liberated N‐acetylneuraminic acid/h/mg of protein. The marked increase of sialidase activity in cerebellar granule cells during the process of differentiation with formation of functional synapses suggests that sialidase enrichment is a marker for the same process
- …
