2,929 research outputs found
The molecular characterisation of type IIA calcium-ATPases in Arabidopsis thaliana
No full textSIGLEAvailable from British Library Document Supply Centre-DSC:DXN031324 / BLDSC - British Library Document Supply CentreGBUnited Kingdo
Dissecting pathways involved in manganese homeostasis and stress in higher plant cells
No full textManganese (Mn) is an essential heavy metal micronutrient in plants having a range of cellular functions. Mn homeostasis involves the coordinated operation of transporters mediating cellular import and export and distribution between cell organelles. Significant progress has been made in identifying transport mechanisms in plants responsible for Mn uptake from the soil, distribution around the plant and compartmentalisation within the cell. These processes are required to ensure that Mn reaches its correct target proteins and also for cellular Mn detoxification. Transporters that have been implicated in Mn transport include members of the P2A-type ATPase, ZIP, CAX, CDF, NRAMP and OPT/YSL families. Here we discuss their roles in the acquisition, distribution and homeostasis of Mn and aspects of their regulation
Two additional type IIA Ca2+-ATPases are expressed in Arabidopsis thaliana: evidence that type IIA sub-groups exist
No full textHigh affinity Ca2+-ATPases play a central role in calcium homeostasis by catalysing the active efflux of calcium from the cytoplasm. This study reports the identification of two additional type IIA (SERCA-type) Ca2+-ATPases from Arabidopsis (AtECA2 and AtECA3), and describes the detailed sequence analysis of these genes in comparison with AtECA1 and other plant and animal Ca2+-ATPases. Southern analysis suggests that each of these genes is present as a single copy and also that there may be a small family of moderately related genes that encode type IIA Ca2+-ATPases in Arabidopsis. Evidence is also provided from RT-PCR that these genes are expressed in Arabidopsis. Hydropathy analysis predicts that the topology of the Arabidopsis type IIA proteins is similar to the animal SERCA proteins. Sequence and phylogenetic analyses suggest that the type IIA Ca2+-ATPases can be further divided into sub-group
Keynote: Jon Gertner
No full textThe symposium will start on the evening of April 16 with a keynote address by Jon Gertner. Jon is a journalist, historian, and feature writer for The New York Times Magazine
as well as the author of the NYTimes bestseller, The Idea Factory. His address will focus on the issue of intellectual property and the ethical questions around the huge amount of human-generated content that large language models use as they are developed
Emerging mechanisms for heavy metal transport in plants
No full textHeavy metal ions such as Cu2+, Zn2+, Mn2+, Fe2+, Ni2+ and Co2+ are essential micronutrients for plant metabolism but when present in excess, these, and non-essential metals such as Cd2+, Hg2+ and Pb2+, can become extremely toxic. Thus mechanisms must exist to satisfy the requirements of cellular metabolism but also to protect cells from toxic effects. The mechanisms deployed in the acquisition of essential heavy metal micronutrients have not been clearly defined although a number of genes have now been identified which encode potential transporters. This review concentrates on three classes of membrane transporters that have been implicated in the transport of heavy metals in a variety of organisms and could serve such a role in plants: the heavy metal (CPx-type) ATPases, the natural resistance-associated macrophage protein (Nramp) family and members of the cation diffusion facilitator (CDF) family. We aim to give an overview of the main features of these transporters in plants in terms of structure, function and regulation drawing on information from studies in a wide variety of organisms. Copyright (C) 2000 Elsevier Science B.V
Jon Mirande eta ironia
No full textLa ironía es un elemento que ha ido siempre unido a la poesía, y especialmente a la poesía moderna.Tras un pequeño repaso a esta en diferentes épocas, se pasa a describir las tres diferentes ironías de Jon Mirande: la intelectual, la social y la filosófica. Todo ello acompañado de ejemplosIrony is an element that has always been united to poetry, and especially to modern poetry. After a small revision of irony in different eras, the author then describes the three different ironies of Jon Mirande: intellectual, social and philosophical irony. All this illustrated with example
Jon Pineda, 32nd Annual ODU Literary Festival
No full textJon Pineda is the author of The Translator\u27s Diary, winner of the Green Rose Prize for Poetry, and BIrthmark, winner of the Crab Orchard Award Series in Poetry Open Competition. His memoir, Sleep in Me, is forthcoming in 2010 from the University of Nebraska Press. He teaches in the low-residency MFA program at Queens University of Charlotte
Increased metal tolerance and bioaccumulation of zinc and cadmium in Chlamydomonas reinhardtii expressing a AtHMA4 C-terminal domain protein
No full textThe use of microalgal biomass for metal pollutant bioremediation might be improved by genetic engineering to modify the selectivity or capacity of metal biosorption. A plant cadmium (Cd) and zinc (Zn) transporter (AtHMA4) was used as a transgene to increase the ability of Chlamydomonas reinhardtii to tolerate 0.2 mM Cd and 0.3 mM Zn exposure. The transgenic cells showed increased accumulation and internalization of both metals compared to wild-type. AtHMA4 was expressed either as the full-length (FL) protein or just the C-terminal (CT) tail, which is known to have metal-binding sites. Similar Cd and Zn tolerance and accumulation was observed with expression of either the FL protein or CT domain, suggesting that enhanced metal tolerance was mainly due to increased metal binding rather than metal transport. The effectiveness of the transgenic cells was further examined by immobilization in calcium alginate to generate microalgal beads that could be added to a metal contaminated solution. Immobilization maintained metal tolerance, while AtHMA4-expressing cells in alginate showed a concentration-dependent increase in metal biosorption that was significantly greater than alginate beads composed of wild-type cells. This demonstrates that expressing AtHMA4 FL or CT has great potential as a strategy for bioremediation using microalgal biomass.</p
Interview with Jon Baskin--May 15, 2015
No full textJon Baskin is co-founder and editor of The Point magazine in Chicago. He is also a graduate student at the University of Chicago's Committee on Social Thought and the author of many essays and works of criticism for venues such as The Los Angeles Review of Books, The Nation, n+1, The New York Observer, BookForum, Salon, and The Point. Earlier in his career he was a fact checker for various magazines, including Popular Science, Inc Magazine, The Atlantic Monthly, and n+1. The interview was conducted at the office of The Point in Chicago on May 15, 2015.1_izzia9z
Jon Sands, 41st Annual ODU Literary Festival
No full textJon Sands is the author of The New Clean (2011), as well as the co-host of The Poetry Gods podcast. His work has been published widely, and anthologized in The Best American Poetry. He’s a youth mentor with Urban Word-NYC, and teaches creative writing for adults at Bailey House in East Harlem (an HIV/AIDS service center). He’s a recent MFA graduate in fiction from Brooklyn College, where his work won the Himan Brown Award for short stories, and he has represented New York City multiple times at the National Poetry Slam. He lives in Brookly
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