1,721,018 research outputs found
Rambach agar and SM-ID medium sensitivity for presumptive identification of Salmonella subspecies I-VI
No full textThe cultural characteristics of 112 Salmonella serovars belonging to subspecies I-VI were examined on Rambach agar and SM-ID medium. Colonies showing the typical red coloration were seen with 100 of 112 serovars assayed on SM-ID, and with 87 of 112 on Rambach agar. Atypical colourless colonies were observed on Rambach agar with ONPG-negative serovars S. Choleraesuis, S. Isangi, S. Typhi S. Worthington and S. Yoff of the subspecies I, S. II 52:d:e,n,x,z, 15 of the subspecies II, S. IV 6,7:Z 4 ,Z 24 :- and S. IV 11:g,Z 51 :- of the subspecies IV, and S. 40:Z 35 :- belonging to S. bongori (V) species. Atypical blue, bluegreen, blue-violet or violet colonies were observed on both media with all the ONPG-positive serovars of the subspecies IIIa (four of four strains) and IIIb (six of six strains) and with one of the two ONPG-positive (out of five) strains of the subspecies VI. Four serovars of S. bongori showed blue-green colonies on Rambach agar and typical red colonies on SM-ID, although they were all ONPG-positive. These results suggest that SM-ID medium is more sensitive than Rambach agar. However, the relatively low sensitivity of both media makes them suitable for use only in association with a traditional selective medium in both medical and environmental bacteriology, as well as for epidemiological purposes
Recent trends in salmonellosis epidemiology
No full textThe taxonomy of the genus Salmonella and the recent trends in the
epidemiology of salmonellosis have been reviewed. Global and Italian
trends in salmonellae infections have been considered focusing
on the application of molecular biology methods to the study
of epidemic isolates, as in the case of S. Wien and the more recent
S. Enteritidis pandemics. Interventions for salmonellosis prevention,
and control measures including new rapid cultural methods
for the detection of salmonellae in foods, have been summarized
[Molecular methods in the epidemiology of gram-negative bacterial infections]
No full textIdentification and typing of bacterial isolates from patients and environment are necessary in order to detect the sources of infections. In recent years different molecular typing methods have been carried out and proved more reliable than methods based on phenotypical characters. We have applied two methods of genotyping, i. e. ribotyping and rrnARDRA (Amplified Ribosomal DNA Restriction Analysis) methods, to the study of different bacterial species. Ribotyping was the first universal method for molecular typing of bacteria. We have succeded both in typing various species of enterobacteria (Salmonella Wien, S. Enteritidis, Shigella sonnei, Proteus spp., Morganella morganii, Providencia spp.) by using different restriction endonucleases and in demonstrating the epidemiological importance of the ribotypes identified in each species. Genotyping by rrnARDRA is an identification method based on the analysis of the electrophoretic patterns obtained after restriction endonucleases digestion of the rrn operon sequences amplified by polymerase chain reaction. This is a universal method, because both the same primers and the same endonucleases are utilized for the identification of Gram-positive as well as Gram-negative bacteria. The identification of a bacterial isolate is performed by comparing its profile (number of bands and molecular weight of the fragments) with those from a data-base of profiles of the various species
Endemic presence of Salmonella bongori 48:z35:- Causing enteritis in children in Sicily
No full textBRIEF NOT
“Attitudini delle studentesse di medicina all’educazione tra pari per la prevenzione dell’AIDS”
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