1,721,021 research outputs found
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Erratum: HIV microarray for the mapping and characterization of HIV-specific antibody responses (Lab on a Chip - Miniaturisation for Chemistry and Biology (2015) 15 (1574-1589))
No full textCorrection for 'HIV microarray for the mapping and characterization of HIV-specific antibody responses' by Daniela Gallerano et al., Lab Chip, 2015, 15, 1574-1589
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Production and characterization of hypoallergenic virus-like nanoparticles shielding full-lenght mutated Art v 1 with membrane-bound murine IL-15 for immunomodulation of allergen-specific T cells
No full textBachelorarbeit - Veterinärmedizinische Universität Wien - 2022Mindestens 95 % der Beifusallergiker sind gegen das Hauptallergen des Beifuspollen, Art v 1, sensibilisiert, was es zu einem interessanten Kandidaten für neue Behandlungen im Rahmen allergenspezifischer Immuntherapie (AIT) macht. Hypoallergene Virus ähnliche Nanopartikel (VNP), die modifizierte Peptidliganden (APL) von Art v 1 beinhalten und murines IL-15 (mIL- 15) auf der Oberflache exprimieren, werden in diesem Zusammenhang als neuer Ansatz für eine sichere Durchführung von AIT als Therapie oder Prävention der Beifusallergie untersucht. Die APL können unterschiedliche T-Zell-Rezeptor-Signale stimulieren und Oberflächenexprimiertes mIL-15 induziert einen Wechsel zur Produktion von Th1-Zytokinen. Für die VNPProduktion wurden HEK293T-Zellen mit drei verschiedenen Plasmiden transfiziert: pMD-OGP (enthalt die gag-pol-Sequenz des Moloney Murine Leukemia Virus (MoMLV)), pk12- MA::Art v 1 (Art v 1 fusioniert mit dem MoMLV-Matrixprotein, MAp15) und pk12-mIL-15-GPI (mIL-15 mit einer GPI-Verankerungssequenz für zusätzliches Th1-Priming). Die zellulare Expression wurde mit Immunhistochemie und Durchflusszytometrie in vorübergehend transfizierten HEK293T-Zellen überprüft. Die erhaltenen VNPs wurden durch wiederholte Runden Ultrazentrifugation aufgereinigt. Mit dem BCA-Protein-Assay wurde der Gesamtproteingehalt der VNPs und mit einem “double-sandwich“ ELISA der Prozentsatz an Art v 1 in den VNPs in Relation zu den WT + mIL-15 VNP quantifiziert. Außerdem wurde ein Western Blot mit SDS-PAGE als weitere biochemische Charakterisierung der VNPs durchgeführt. Die Allergenität der VNP-Präparate wurde mit Art v 1-spezifischen IgE sensibilisierten Ratten-Basophilen-Leukämiezellen getestet. Die VNP beinhalteten die APL des His-markierten Art v 1 und exprimierten mIL-15 auf ihrer Oberflache. Die Hypoallergenität einiger VNP sowie die korrekte Expression der Plasmide und Größe der rekombinanten Proteine wurde bestätigt. Zusammenfassend lasst sich sagen, dass VNPs, welche Art v 1 in voller Länge beinhalten und mIL-15 auf ihrer Oberflache exprimieren, erfolgreich hergestellt und biochemisch charakterisiert wurden. Diese neuen Plattformen können nun in vitro und in vivo auf ihre immunmodulatorische Wirkung auf die allergenspezifische Th-Zytokin-Produktion untersucht werden.Bachelor thesis - University of Veterinary Medicine Vienna - 2022At least 95 % of mugwort allergic patients are sensitized against the major mugwort pollen allergen Art v 1, making it an interesting candidate for new treatments based on Allergenspecific immunotherapy (AIT). Hypoallergenic virus-like nanoparticles (VNP) encasing altered peptide ligands (APL) of Art v 1 and surface expressing murine IL-15 (mIL-15) are therefore being examined as a new approach to safely perform AIT. The APLs can cause differential Tcell receptor signals and surface-expressed mIL-15 also induces a shift towards Th1 cytokine production. HEK293T cells were transfected with three different plasmids, pMD-OGP (containing a gag-pol sequence of Moloney Murine Leukemia Virus (MoMLV)), pk12- MA::Art v 1 (Art v 1 fused to the MoMLV matrix protein, MAp15) and pk12-mIL-15-GPI (mIL- 15 with a GPI-anchor attachment sequence, for additional Th1 priming) for VNP production. The proper cellular expression was checked with flow cytometric staining on transiently transfected HEK293T cells. The obtained VNPs were purified with successive rounds of ultracentrifugation washing steps. With the BCA protein assay, the total protein content of the VNPs and with a double-sandwich ELISA, the percentage of Art v 1 inside the VNPs were quantified in relation to the WT + mIL-15 VNP. Further biochemical characterization of the VNPs was performed through Western Blot with SDS-PAGE. The hypoallergenicity of the VNP preparation was tested using Art v 1-specific-IgE sensitized rat basophil leukemia cells as the readout. The VNPs shielded full-length his-tagged APLs of Art v 1 and expressed mIL-15 on their surface. The hypoallergenicity of some VNPs, the proper expression of the plasmids and the size of recombinant proteins were confirmed. In conclusion, the VNPs shielding the fulllength his-tagged APLs of Art v 1 and expressing mIL-15 on their surface, were successfully produced and biochemically characterized. These new platforms can now be studied in vitro and in vivo for their immunomodulatory effects on allergen-specific Th cytokine production
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
HIV microarray for the mapping and characterization of HIV-specific antibody responses
No full textWe used the microarray technology to develop chips containing a comprehensive set of proteins and peptides covering the proteome of HIV-1 clade C, which is the HIV-1 subtype that causes the majority of infections worldwide. We demonstrate that the HIV microarray allows simultaneous, sensitive and specific detection of antibody responses for the major immunoglobulin classes (IgG, IgA, IgM, IgE) and subclasses (IgG1-4) with minute amounts of serum samples towards a large number of HIV antigens and peptides. Furthermore, we show that the HIV chip can be used for the monitoring of antibody responses during the course of the disease and during treatment. The HIV microarray should be useful to study antibody responses to multiple HIV antigens and epitopes in HIV-infected patients to explore pathomechanisms of the disease, for diagnosis and for monitoring of treatment and of vaccine trials
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
- …
