6,787 research outputs found
CHARACTERIZATION OF THE GROUND STATE OF THE (R=Ne, Ar, Kr) COMPLEXES
No full textAuthor Institution: Laser Spectroscopy Facility, Department of Chemistry, The Ohio State UniversityInformation characterizing the state of the complexes has been obtained from two complimentary experimental techniques. The spin-vibronic energy levels have been determined by wavelength resolved fluorescence subsequent to laser excitation of specific vibronic levels of the state. Both moderate and high resolution laser induced fluorescence has also been used to characterize the state owing to the observation of ``hot'' bands from excited spin-vibronic levels of . The experimental data have been used to construct very simple models for the ground state potential energy surface for each complex. These models show that the most stable conformation for each complex is linear H-bonded, but the barrier to isomerization to the S-bonded complex is quite low. The overall bonding is somewhat weaker and more isotropic than the corresponding hydroxyl complexes
Beijing xi pai pi ying yi zhen
No full text"Beijing xi pai pi ying yi zhen" zhu yao nei rong bao kuo: Jing xi xia wei dian pi ying de bao xiang yu jue se, Beijing xi pai lu shi dang dai pi ying jing xuan, fu lu: pi ying zhuan yong ming ci jie sh
Wellesly Sh. W. to Mr. James Meredith (2 October 1962)
Get PDFSigned by Wellesly Sh. W.https://egrove.olemiss.edu/mercorr_pro/1531/thumbnail.jp
Characterization of essential sulfhydryl groups of rat renal Na(+)-Pi cotransporter
No full text Sulfhydryl (SH) groups are essential for the function of the Na(+)-Pi cotransporter of renal brush-border membrane (BBM) as determined by inhibition of Na(+)-Pi cotransport by HgCl2. Recent studies suggest that essential SH groups may be present on the cytoplasmic side of the BBM. We used various maneuvers to differentiate between external and internal SH groups on the Na(+)-Pi cotransporter in renal BBM vesicles (BBMV). The inhibitory potency of p-chloromercuriphenylsulfonic acid (PCMBS), a poorly permeable SH reagent, was about one-half that of p-chloromercuribenzoic acid (PCMB), a highly permeable reagent (half-maximal inhibitory concentrations of 625 and 350 microM, respectively). 5,5'-Dithio-bis-(2-nitrobenzoic acid) (DTNB) and N-ethylmaleimide (NEM) were additive to HgCl2 for inhibition of Pi transport. The highly permeable NEM gave a more pronounced additive effect (+30%) than the less permeable DTNB (+15%). When the intravesicular pH (pHi) and extravesicular pH (pHo) were varied independently, NEM (which reacts mainly at an alkaline pH) inhibited Pi transport only at pHi = 8.5, regardless of pHo. When internal SH groups were blocked by NEM at pH 8.5, PCMB and PCMBS produced similar additive effects. The binding of 14C-labeled phosphonoformic acid was inhibited by both reagents and to the same extent. Both PCMB and PCMBS increased 32Pi efflux from BBMV. The findings are consistent with the presence of essential SH groups on the cytoplasmic side of the BBM, with possible conformational changes induced by modification of the external SH groups. </jats:p
An initial investigation of S and SH with angle resolved photoelectron spectroscopy using synchrotron radiation
No full textAn initial investigation of the photoionization behaviour of S atoms and SH radicals has been made in the gas-phase using angle resolved photoelectron spectroscopy (PES) with radiation from the Elettra synchrotron as the photon source. Angular distributions and relative photoionization cross-sections have been measured for S and SH as a function of photon energy from threshold (similar to10.3 eV) to 21.64 eV. The ionizations considered are S+(S-4)<--S(P-3), S+(D-2)<--S(P-3) and S+(P-2)<--S(P-3), and SH+(X(3)Sigma(-), v(+) = 0)<--SH(X(2)Pi, v" = 0), SH+(a(1)Delta, v(+) = 0)<--SH(X(2)Pi, v" = 0) and SH+(b(1)Sigma(+), v(+) = 0)<--SH(X(2)Pi, v" = 0). This study of S and SH follows a recent investigation on atomic oxygen where the feasibility of angle resolved PES measurements on reactive intermediates at the Elettra synchrotron source was demonstrated, and further studies on small molecular radicals were proposed
PI influx in dopaminergic cells.
No full text<p>SH-SY5Y cells were treated with aS/DHA oligomers and the number of PI-positive cells was calculated as a percentage of the total counterstained cells. Quantitation (n = 3 cultures, with 5 fields analyzed per culture, 250-300 cells per field; error bars indicated the S.E.) indicated that aS/DHA oligomers increased the percentage of PI-positive cells. Statistical significance was calculated by one-way ANOVA (p < 0.05) compared with mock control or monomeric aS. </p
``ANOMALOUS'' RO-VIBRATIONAL INTENSITIES IN THE BANDS OF
No full text J.B. Burkholder, P.D. Hammer, C.J. Howard, A.G. Maki, G. Thompson, and C. Chackerian, Jr., J. Mol. Spectrosc. 124, 137 (1987). C. Chackerian, Jr., G. Guelachvili, A. Lopez-Pineiro. and R.H. Tipping, J. Chem. Phys. 90. 641 (1989). D.D. Nelson, Jr., A. Schiffman, D.J. Nesbitt and J.D. Yaron, J. Chem. Phys. (to be published).Author Institution: Laboratoire d'Infrarouge, Universite de Paris-Sud.; Laboratoire d'Infrarouge, NASA-Ames Research CenterRelative line Intensities of p- and r- branch transitions of diatomic radical molecules (ClO, NH, OH) have recently1-3 been used along with the theory of the Herman-Wallis effect to obtain estimates of electric dipole vibrational transition moments. Of the first and second row diatomic hydrides, ab initio calculations predict SH to exhibit the largest H.W. effect. Indeed, our spectra confirm a very large effect, and we present here an attempt at a quantitative analysis of the Herman-Wallis intensity perturbation in SH
DISPERSED FLUORESCENCE SPECTRA AND GROUND STATE POTENTIAL OF COMPLEXES (R=Ne, Ar AND Kr)
No full textM. -L. Dubenet, D. Flower and J.M. Hutson, {J. Phys. Chem}. \textbf{94}, 7602(1991)Author Institution: Laser Spectroscopy Facility, Department of Chemistry, The Ohio State UniversityThe dispersed fluorescence spectra of R.SH have been observed following laser excitation of specific excited vibronic levels. There are three possible emission mechanisms; (1)re-emission from the pumped level, (2)relaxation to lower vibrational levels and emission therefrom, and (3) a dissociation mechanism: . This latter mechanism has little contribution because the lifetime of the excited state SH is very short ; therefore more of the SH will dissociate rather then emitting photons. Mechanisms (1)and (2) can be separated by careful comparison of spectra from adjacent vibrational levels. From the resulting analysis much can be learned about the state’s vibrational structure. The ground states of R-SH can be described as near the free rotor limit. Most of the observed emission features are terminated on the internal SH-rotor levels in the ground state but in Ne-SH and Ar-SH, transitions to levels associated with excitation of the R-SH stretch are also observed. Due to the experimental resolution one does not resolve the P structure in the ground state. Using the dispersed flurescence data, the ground state angular potential can be fit to the model developed by Dubernet {et al}. The details of the analysis will be presented
The synthetic chemoattractant peptide, Trp-Lys-Tyr-Met-Val-D-Met, enhances monocyte survival via PKC-dependent Akt activation
No full textPreviously, we showed that Trp-Lys-Tyr-Met-Val-D-Met (WKYMVm) stimulates superoxide generation and chemotactic migration in monocytes and neutrophils. In this study, we examined the effect of WKYMVm on monocyte survival. Serum starvation-induced monocyte death was attenuated in the presence of WKYMVm, which was abated when the cells were preincubated with LY294002, suggesting the involvement of phosphoinositide-3-kinase (PI 3-kinase) in the peptide-induced monocyte survival. WKYMVm stimulated ERK and Akt activity via PI 3-kinase activation in monocytes. We also investigated the signaling pathway of WKYMVm-induced ERK and Akt activation. The WKYMVm-induced ERK activation was PI 3-kinase-dependent but PKC-independent. However, Akt activation by WKYMVm was dependent not only on PI 3-kinase but also on the PKC pathway. When monocytes were incubated with WKYMVm, caspase-3 activity, which is important for cell death, was inhibited. Pretreatment of the cells with LY294002, GF109203X, and Go 6976 but not PD98059 blocked WKYMVm-induced monocyte survival and caspase-3 inhibition. In summary, the novel chemoattractant WKYMVm enhances monocyte survival via Akt-mediated pathways, and in this process, PKC and PI 3-kinase act upstream of Akt.X1111sciescopu
FLZ protected SH-SY5Y (APPwt/swe) cells from apoptosis.
No full text<p>SH-SY5Y (APPwt/swe) cells were grown in “stimulating medium” containing 50% DMEM, 50% Opti-MEM, 0.5% FBS, 200 µg/ml G418 and 10 mM butyric acid sodium salt for 12 h to induce the transgene expression. FLZ (0.1, 1 and 10 µM) were incubated with cells for 24 h. (A) Cell viability of SH-SY5Y (APPwt/swe) cells. (B) Apoptosis of SH-SY5Y (APPwt/swe) cells measured by flow cytometry with Annexin-V/PI staining. Bar chart is the statistical of the sum of early and late cell apoptosis. (C) Caspase-3 activity of SH-SY5Y (APPwt/swe) cells. Results were expressed as mean ± SD from 6 independent experiments. **<i>P</i><0.01 <i>vs</i>. Neo SH-SY5Y cells, <sup>#</sup><i>P</i><0.05, <sup>##</sup><i>P</i><0.01 <i>vs</i>. solvent-treated SH-SY5Y (APPwt/swe) cells.</p
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