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    Congruent patterns of lineage diversity in two species complexes of planktonic crustaceans, Daphnia longispina (Cladocera) and Eucyclops serrulatus (Copepoda), in East European mountain lakes

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    Hamrová, Eva, Krajicek, Martin, Karanovic, Tomislav, Černý, Martin, Petrusek, Adam (2012): Congruent patterns of lineage diversity in two species complexes of planktonic crustaceans, Daphnia longispina (Cladocera) and Eucyclops serrulatus (Copepoda), in East European mountain lakes. Zoological Journal of the Linnean Society 166 (4): 754-767, DOI: 10.1111/j.1096-3642.2012.00864.x, URL: http://dx.doi.org/10.1111/j.1096-3642.2012.00864.

    FIGURE 3 in A new Central European species of the Daphnia curvirostris complex, Daphnia hrbaceki sp. nov. (Cladocera, Anomopoda, Daphniidae)

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    FIGURE 3. Comparison of postabdominal claws. Arrows indicate second (middle) pecten of spinules or teeth of postabdominal claw. A. Daphnia hrbaceki, adult female (K). B. D. hrbaceki, adult female from laboratory culture (K). C. D. hrbaceki, adult female (RB). D. D. hrbaceki, adult female from laboratory culture (K); detail of middle pecten. E. D. hrbaceki, adult male from laboratory culture (K). F. Daphnia sp. (morphotype FLO9), adult female (FL). G. D. minnehaha, adult female (ELA).Published as part of Juračka, Petr Jan, Kořínek, Vladimír & Petrusek, Adam, 2010, A new Central European species of the Daphnia curvirostris complex, Daphnia hrbaceki sp. nov. (Cladocera, Anomopoda, Daphniidae), pp. 1-22 in Zootaxa 2718 on page 9, DOI: 10.5281/zenodo.19978

    Natural distribution and co-infection patterns of microsporidia parasites in the Daphnia longispina complex

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    Microsporidia are intracellular parasites, frequently infecting the planktonic crustacean Daphnia. Questioning the ability to detect and identify microsporidia with conventional microscopic techniques, we applied molecular methods in order to investigate the distribution and co-infection patterns of this parasite among 8 communities of the Daphnia longispina hybrid complex. Eight microsporidian taxa were detected, including 3 that previously had not been characterized genetically. Microsporidian communities from nearby lakes were found to be more similar to each other, apparently due to short distance dispersal via secondary hosts. Moreover, we detected seasonal (but not interannual) changes in microsporidian community structure. With some microsporidia being host-specific, these changes might have resulted from seasonal changes in host taxon and clonal composition. The 2 dominant and closely related parasite species were found mainly in single infections, whereas another pair of related microsporidians was found predominantly in co-infections; suggesting species-level differences in the ability to colonize infected hosts. By applying molecular methods, we were not only able to unambiguously identify parasite taxa but also to reveal multiple infections that otherwise would have remained undetected. Given the increased level of accuracy and sensitivity, we highly recommend molecular approaches in future parasite surveys of Daphnia infections

    (Gastropoda: Pulmonata)

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    Figure 1. Comparison of types of (A) Helix lucorum (neotype, Zoological Museum Copenhagen) and (B) H. straminea (probable syntype, MHNG 18135).Published as part of Korábek, Ondřej, Juřičková, Lucie & Petrusek, Adam, 2014, Resurrecting Helix straminea, a forgotten escargot with trans-Adriatic distribution: first insights into the genetic variation within the genus Helix (Gastropoda: Pulmonata), pp. 72-91 in Zoological Journal of the Linnean Society 171 (1) on page 74, DOI: 10.1111/zoj.12122, http://zenodo.org/record/472049

    Figure 1 in Congruent patterns of lineage diversity in two species complexes of planktonic crustaceans, Daphnia longispina (Cladocera) and Eucyclops serrulatus (Copepoda), in East European mountain lakes

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    Figure 1. Map of the sampled Eastern European mountain ranges (Bje, Bjelasica; Dur, Durmitor; Pir, Pirin; Pro, Prokletije; Ret, Retezat; Ril, Rila; Sar, Šar Planina; Tat, Tatra Mountains; Tre, Treskavica; Zel, Zelengora). The main mountain regions are differentiated by shading: the Carpathians in dark grey, Macedonian-Thracian massif in white, and Dinaric Alps in light grey. Countries are indicated by two-letter codes (see Table 1).Published as part of Hamrová, Eva, Krajicek, Martin, Karanovic, Tomislav, Černý, Martin & Petrusek, Adam, 2012, Congruent patterns of lineage diversity in two species complexes of planktonic crustaceans, Daphnia longispina (Cladocera) and Eucyclops serrulatus (Copepoda), in East European mountain lakes, pp. 754-767 in Zoological Journal of the Linnean Society 166 (4) on page 758, DOI: 10.1111/j.1096-3642.2012.00864.x, http://zenodo.org/record/540925

    Figure 3 in Congruent patterns of lineage diversity in two species complexes of planktonic crustaceans, Daphnia longispina (Cladocera) and Eucyclops serrulatus (Copepoda), in East European mountain lakes

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    Figure 3. Relationship of eight clades of the Eucyclops serrulatus complex, assessed by Bayesian inference of phylogeny, and haplotype variation of the 12S rRNA gene within clade I. The phylogenetic tree was based on the 1299-bp-long alignment consisting of fragments of mitochondrial genes for 12S rRNA and cytochrome b, and the nuclear gene for 18S rRNA. The scale bar represents genetic distance; numbers at nodes indicate branch support (as posterior probabilities). Haplotype network representing the variation within clade I is based on 43 sequences of the 383-bp-long 12S rDNA fragment. Individuals from the three main mountain regions are indicated by different shading (as in Figs 1, 2) in both tree and network: the Carpathians in dark grey (N = 24), Macedonian-Thracian massif in white (N = 9), and Dinaric Alps in light grey (N = 26). Mountain range abbreviations: Bje, Bjelasica; Dur, Durmitor; Pir, Pirin; Pro, Prokletije; Ret, Retezat; Ril, Rila; Sar, Šar Planina; Tat, Tatra Mountains; Tre, Treskavica; Zel, Zelengora. Countries are indicated by two-letter codes (see Table 1).Published as part of Hamrová, Eva, Krajicek, Martin, Karanovic, Tomislav, Černý, Martin & Petrusek, Adam, 2012, Congruent patterns of lineage diversity in two species complexes of planktonic crustaceans, Daphnia longispina (Cladocera) and Eucyclops serrulatus (Copepoda), in East European mountain lakes, pp. 754-767 in Zoological Journal of the Linnean Society 166 (4) on page 762, DOI: 10.1111/j.1096-3642.2012.00864.x, http://zenodo.org/record/540925

    Figure 2 in Congruent patterns of lineage diversity in two species complexes of planktonic crustaceans, Daphnia longispina (Cladocera) and Eucyclops serrulatus (Copepoda), in East European mountain lakes

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    Figure 2. Sequence variation of the 528-bp-long fragment of the 12S rRNA gene within the Daphnia longispina complex from lakes of the studied East European mountain ranges. This is shown in a maximum likelihood tree (A) consisting only of sequences from the studied region (each haplotype represented once per lake), and in a parsimony network (B) of haplotypes of D. longispina s.s., amongst which 63 reference sequences from other European localities were also included. Three main mountain regions from this study are differentiated by shading: the Carpathians in dark grey, Macedonian-Thracian massif in white, and Dinaric Alps in light grey. Haplotypes from other localities, only included in the network, are enclosed by dashed lines. Mountain range abbreviations: Bje, Bjelasica; Dur, Durmitor; Pir, Pirin; Pro, Prokletije; Ret, Retezat; Ril, Rila; Tat, Tatra Mountains; Tre, Treskavica; Zel, Zelengora. Countries are indicated by two-letter codes (see Table 1).Published as part of Hamrová, Eva, Krajicek, Martin, Karanovic, Tomislav, Černý, Martin & Petrusek, Adam, 2012, Congruent patterns of lineage diversity in two species complexes of planktonic crustaceans, Daphnia longispina (Cladocera) and Eucyclops serrulatus (Copepoda), in East European mountain lakes, pp. 754-767 in Zoological Journal of the Linnean Society 166 (4) on page 760, DOI: 10.1111/j.1096-3642.2012.00864.x, http://zenodo.org/record/540925

    Genetic variation in North American crayfish species introduced to Europe and the prevalence of the crayfish plague pathogen in their populations

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    - ABSTRACT (IN ENGLISH) - Biological invasions by crustaceans represent a serious threat for native species in Europe. In my thesis I focus on non-indigenous freshwater crayfish introduced to Europe and their parasite Aphanomyces astaci, the pathogen of the crayfish plague. The thesis consists of four already published first-author papers (chapters I, II, IV and V), two first-author manuscripts (chapters III and VI), and one paper which I co-authored (chapter VII). The first part (chapters I-V) focuses on genetic variation in North American crayfish introduced to Europe. We showed that in two crayfish species, both successful invaders in Europe, genetic variation differs significantly, reflecting their different colonization histories on the continent. The spiny-cheek crayfish Orconectes limosus was likely introduced to Europe just once, in small numbers (90 individuals). Variation at the mitochondrial DNA (mtDNA) level in the spiny-cheek crayfish in Europe is much lower compared to North America (chapter I), although some variation was revealed by nuclear markers in its Central European populations (chapter II). In contrast, the signal crayfish Pacifastacus leniusculus was introduced to Europe several times, in large numbers. Its European populations are highly diverse genetically and belong to a single..

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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