186,326 research outputs found
Studio e caratterizzazione molecolare di rotavirus circolanti in Albania ed in differenti aree italiane
No full textI Rotavirus sono una delle cause più importanti di gastroenterite nei bambini con età minore di 5 anni. L’analisi dei genotipi G e P dei Rotavirus in circolazione è di fondamentale importanza nella valutazione della appropriatezza della vaccinazione di massa dei bambini in tutto il mondo. Nel complesso, 592 campioni di feci sono stati raccolti a Tirana (Albania), nel Salento (Sud Italia), e in tre diversi ospedali di Roma (Italia Centrale). Dei campioni totali, il 31% è risultato positivo ai Rotavirus in Albania, il 78% nel Salento, e il 38% a Roma. I campioni raccolti a Tirana e Roma sono stati G-P tipizzati, mentre i campioni raccolti nel Salento solo G tipizzati. Complessivamente, le combinazioni più frequentemente isolate in Italia sono state G4-P[8] (48%), G1-P[8] (28%), e G2-P[4] (9%), mentre in Albania sono state G9-P[8] (il 77%), G4-P[8] (9%), G1-P[8] (7%) e G4-P[4] (3%). L’analisi filogenetica è stata effettuata anche per valutare la parentela genetica dei ceppi.Rotaviruses are one of the most important causes of gastroenteritis in children under 5 years old. Analysis of G and P rotavirus genotypes in circulation is crucial in evaluating the appropriacy of mass vaccination of children worldwide. Overall, 592 stool samples were collected in Tirana (Albania), the Salento peninsula (South Italy), and three different hospitals in Rome (Central Italy). Of the total samples, 31% were rotavirus positive in Albania, 78% in the Salento, and 38% in Rome. The samples collected in Tirana and Rome were G–P typed, whereas the samples collected in the Salento were only G typed. Overall, in Italy the most frequent combinations were G4P[8] (48%),G1P[8] (28%), and G2 P[4] (9%); in Albania they were G9 P[8] (77%), G4 P[8] (9%), G1 P[8] (7%), and G4 P[4] (3%). Phylogenetic analysis was also performed to assess the genetic relatedness of the strains
A strategy to increase the specificity of Syber Green I qRT-PCR in hepatitis A detection
No full textVarious methods for the recovery and detection of HAV have been suggested, and molecular tests have recently provided
an effective replacement for the traditional methods. Real-time RT-PCR technology offers many advantages over
conventional RT-PCR in terms of rapidity and specificity. Most procedures are based on the TaqMan chemistry, but
some researchers have used the SYBR Green I approach, which is less expensive and simpler to carry out. However
the formationof primer-dimers needs to be distinguished from specific products through a melting curve analysis. This
study focused on a strategy to increase the specificity of Syber Green I chemistry, thus nullifying the primer-dimers
interference. To this end, forward and reverse primers were specially designed for hairpin loop formation, a strategy
widely used to improve the specificity and the efficiency of PCR. Two different concentrations of primers were assayed
(200 nM and 400 nM) in a one-step, real-time RT-PCR procedure, evaluating the specificity of the amplicons and the
optimization of the real-time protocol. We demonstrated that this approach can increase the specificity of the Syber
Green I qRT-PCR performance with a good reproducibility of the method. Because of the simplicity of the assay and
the lower costs involved, this procedure could be a valid alternative to HAV monitoring from environmental matrices
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Ottimizzazione degli interventi processistici e delle pratiche gestionali degli impianti di trattamento reflui finalizzate alla rimozione dei virus
No full textAppropriate Similarity Measures for Author Cocitation Analysis
No full textWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
No full textWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Dr. Edward P. Wimberly, ITC, July 2011
No full textThis video is a conversation with Dr. Edward P. Wimberly. Dr. Wimberly talks about his book, "No Shame in Wesley's Gospel: A Twenty-First Century Pastoral Gospel". Brad Ost, AUC Woodruff Library, is the interviewer
Author Rights and Scholarly Publishing
No full textOriginally posted at
http://blog.library.gsu.edu/2014/10/24/author-rights-and-scholarly-publishing/</p
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