74 research outputs found
Archaeology in the community - educational aspects: Greece: a case-study
No full textHeritage education in Greece reproduces and reassures the individual, social and national self. My purpose is to discuss the reasons for this situation and, by giving account of the recent developments in Western Europe and the new Greek initiatives, to improve the study of the past using non-traditional school education. In particular. Local History projects through the Environmental Education optional lessons allow students to approach the past in a more natural way, that is through the study of the sources and first hand material. The community itself is involved in the projects either as a geographical place where the children's activities are located and referred to or as a source of a different perspective which enhances the school's world view. Museum projects are not everywhere equally profitable in Greece, especially where they are not combined with other activities in general school planning. Being a teacher in a Greek school I started to set up similar projects within Environmental Education, in order to articulate a syllabus which might work as a model for my colleagues all over Greece. My project put emphasis - as New History did - on the ability of (and the necessity for) children to undertake small-scale academic research including Archaeology. It emphasised also the interaction between the community and schools, and the advance of long-term education for sustainable development. My involvement in the educational affair stimulated official and/or unofficial improvements which fit well with cross-developments announced in the Greek educational system as part of a very recent tendency in the socio-political sphere to alter the current situation. Children as not only long-term, but also short-term mediators of knowledge and attitudes constitute a major factor for change within the community
The isolation and analysis of the hap genes of Aspergillus nidulans
No full textTypescript (photocopy)Thesis (MSc) -- University of Melbourne, Faculty of Science, 1997Includes bibliographical references (leaves 123-141)The 5' regulatory region of the amdS gene of Aspergillus nidulans, which encodes an acetamidase required for growth on acetamide as a carbon and nitrogen source, contains a CCAAT sequence required for setting the basal level of transcription. Mobility shift assays have identified a factor in A. nidulans nuclear extracts that binds specifically to this CCAAT sequence. In Saccharomyces cerevisiae, the HAP3 and HAP5 genes encode components of a highly conserved multi subunit complex which is able to bind CCAAT sequences. The identification, cloning and sequencing of genes from A. nidulans with homology to HAP3 and HAP5, known as hapC and hapE respectively, is described here. The predicted amino acid sequences of the proteins encoded by the hapC and hapE genes share extensive sequence identity to conserved regions in HAP3 and HAP5 respectively. Furthermore, they both show identity to the histone-fold motif, a motif used widely as a means for protein-protein and DNA- protein interactions. A haploid carrying a hapC deletion has been created and is viable, but grows poorly on all media tested. This null mutant grows especially poorly on acetamide as a sole carbon and nitrogen source, indicating that hapC plays a role in amdS expression. In agreement with this notion, the hapC deletion results in reduced levels of amdS expression, particularly under conditions of carbon limitation. Nuclear extracts prepared from the hapC deletion mutant show no CCAAT specific binding to the amdS or gatA promoter, indicating that hapC encodes a component of the complex binding at this sequence. In the presence of the hapC deletion growth on acetamide and amd
Hydration and nanoscale aqueous morphologies in thermoresponsive double hydrophilic block copolymers with drug encapsulation potential
No full textBy synergy of Fourier transform infrared (FTIR) spectroscopy and small angle neutron scattering (SANS) experiments, we obtain a thorough understanding of the nanoscale aqueous assemblies of novel thermoresponsive and double hydrophilic poly(N-isopropylacrylamide)-block-poly(oligo ethylene glycol methyl ether acrylate) (PNIPAM-b-POEGA) copolymers. [1] The purely hydrophilic POEGA block maintains the stealth properties like PEO, [2] but adopts a more complex comb-shaped topology. Assemblies of this diblock copolymer have succeeded in encapsulating the hydrophobic drug indomethacin. [3] We focus on the influence of temperature as external stimulus and block length asymmetry, in order to broaden our understanding of the self-assembly of the drug delivery scaffolds. We resolve differences in morphologies and molecular hydration characteristics in assemblies of symmetric and asymmetric PNIPAM-b-POEGA diblock copolymers. [1] Upon heating, block-length dependent nanostructural transformations from hierarchical assemblies to more well-defined spherical morphologies are evidenced by SANS. Differences in the strength and/or amount of hydrogen bonding and hydrophobic interactions lead to distinct morphological transformations expressed by variations in cluster compactness and hydration. The methyl side group hydration, as evidenced by FTIR, sensitively depends on the PNIPAM block length. The understanding of these assemblies allows to optimize these scaffolds for drug encapsulation protocols. [1] Vagias, A; Papagiannopoulos, A; Kreuzer, L. P.; Giaouzi, D; Busch, S.; Pispas, S., Müller-Buschbaum, P. Effects of Polymer Block Length Asymmetry and Temperature on the Nanoscale Morphology of Thermoresponsive Double Hydrophilic Block Copolymers in Aqueous Solutions. Macromolecules 2021, 54,7298-7313 (with Journal Cover)[2] Salmaso, S; Caliceti, P. Stealth Properties to Improve Therapeutic Efficacy of Drug Nanocarriers. Journal of Drug Delivery 2013, 2013, 374252, 1-19[3] Giaouzi, D.; Pispas, S. Synthesis and Self-Assembly of Thermoresponsive Poly(N-Isopropylacrylamide)-b-Poly(Oligo Ethylene Glycol Methyl Ether Acrylate) Double Hydrophilic Block Copolymers. J. Polym. Sci., Part A: Polym. Chem. 2019, 57, 1467− 147
A computational index derived from whole-genome copy number analysis is a novel tool for prognosis in early stage lung squamous cell carcinoma
No full textAbstractSquamous cell carcinoma of the lung is remarkable for the extent to which the same chromosomal abnormalities are detected in individual tumours. We have used next generation sequencing at low coverage to produce high resolution copy number karyograms of a series of 89 non-small cell lung tumours specifically of the squamous cell subtype. Because this methodology is able to create karyograms from formalin-fixed paraffin-embedded material, we were able to use archival stored samples for which survival data were available and correlate frequently occurring copy number changes with disease outcome. No single region of genomic change showed significant correlation with survival. However, adopting a whole-genome approach, we devised an algorithm that relates to total genomic damage, specifically the relative ratios of copy number states across the genome. This algorithm generated a novel index, which is an independent prognostic indicator in early stage squamous cell carcinoma of the lung
Dynamics and cosmological evolution in Λ -varying cosmology
No full textWe study the dynamical properties of a large body of varying vacuum cosmologies for which dark matter interacts with vacuum. In particular, performing the critical point analysis we investigate the existence and the stability of cosmological solutions which describe de-Sitter, radiation and matter dominated eras. We find several cases of varying vacuum models that admit stable critical points, hence they can be used in describing the cosmic history. © 2020, The Author(s)
Vectorizing higher-order masking
No full textContains fulltext :
191739pre.pdf (Author’s version preprint ) (Open Access)
Contains fulltext :
191739pos.pdf (Author’s version postprint ) (Open Access)
Contains fulltext :
191739pub.pdf (Publisher’s version ) (Open Access
Appropriate extent of surgery for aspirin‐exacerbated respiratory disease
No full textThe current literature lacks strong guidelines regarding surgical management of patients with aspirin-exacerbated respiratory disease (AERD), who present with the clinical triad of chronic rhinosinusitis with nasal polyposis (CRSwNP), bronchial asthma, and aspirin/nonsteroidal anti-inflammatory drug intolerance. To further define the effectiveness of sinus surgery in treating AERD patients, this review article discusses current evidence regarding outcomes associated with more extensive surgery, the benefits of frontal sinus surgery on polyposis, and the role of Draf III intervention. Numerous studies suggest that Draf III frontal sinusotomy may be an efficacious early intervention due to increased neo-ostial patency and subsequent distribution of topical therapies. Future studies that further investigate the efficacy and safety of extensive surgery in AERD patients are warranted
Dynamics in varying vacuum Finsler–Randers cosmology
No full textIn the context of Finsler–Randers theory we consider, for the first time, the cosmological scenario of the varying vacuum. In particular, we assume the existence of a cosmological fluid source described by an ideal fluid and the varying vacuum terms. We determine the cosmological history of this model by performing a detailed study on the dynamics of the field equations. We determine the limit of General Relativity, while we find new eras in the cosmological history provided by the geometrodynamical terms provided by the Finsler–Randers theory. © 2020, The Author(s)
Cosmological singularities and analytical solutions in varying vacuum cosmologies
No full textWe investigate the dynamical features of a large family of running vacuum cosmologies for which Λ evolves as a polynomial in the Hubble parameter. Specifically, using the critical point analysis we study the existence and the stability of singular solutions which describe de-Sitter, radiation and matter dominated eras. We find several classes of Λ (H) cosmologies for which new analytical solutions are given in terms of Laurent expansions. Finally, we show that the Milne universe and the Rh= ct model can be seen as perturbations around a specific Λ (H) model, but this model is unstable. © 2018, The Author(s)
Dynamics of Einstein–Aether scalar field cosmology
No full textWe study the cosmological evolution of the field equations in the context of Einstein–Aether cosmology by including a scalar field in a spatially flat Friedmann–Lemaître–Robertson–Walker spacetime. Our analysis is separated into two separate where a pressureless fluid source is included or absent. In particular, we determine the critical points of the field equations and we study the stability of the specific solutions. The limit of general relativity is fully recovered, while the dynamical system admits de Sitter solutions which can describe the past inflationary era and the future late-time attractor. Results for generic scalar field potentials are presented while some numerical behaviours are given for specific potential forms. © 2019, The Author(s)
- …
