1,721,005 research outputs found
Transgenic livestock technologies
No full textRecombinant DNA technology and gene transfer were set about forty years ago and since then have provided powerful tools for the production of biological molecules, the development of new varieties of crop and for generating key animal models in biomedical research.
Following the synthesis of recombinant insulin, many other pharmacological proteins have been produced by expressing heterologous genes in micro-organisms, plants and animals.
The application of gene transfer or “transgenesis” to animals has been developed mainly in mouse, while its use for generating transgenic livestock has encountered unanticipated resistances originated by social, economical and technical consideration. Apart from public concern and social consideration about the release of Genetically Engineered products on the market and GE-animals into the environment, that will not be discussed in this chapter, the development of transgenic livestock has been slow due to the technical difficulties faced in transferring this technology from mouse to large domestic animals and by the initial discouraging results, outlining the difficulties in obtaining the expected effect upon a genetic modification.
This review will survey the scientific advancement making the generation of GE swine and ruminants more accessible in the last few years, positively influencing the technical and, as a consequence, the economical issues related to their development
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Efficient generation of Gal-KO cell lines for SCNT by selection with biotin-conjugated IB4-lectin attached to streptavidin-coated magnetic beads.
No full textVariations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Expression of green fluorescent protein in pig blastocysts obtained by somatic cell nuclear transfer
No full textAnalysis of transgene expression in transfected pig cell to be used as donor in nuclear transfer
No full textSus scrofa V2G: locus transgenico ad alta efficienza di integrazione e di espressione a lungo termine di transgeni nel suino
No full textThe present invention concerns the field of gene integration and durable gene expression in specific genomic sites of transgenic animals suitable for the generation of tools for biomedical research and biotechnological applications
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