565 research outputs found
Creation of an endA mutant strain in Pseudomonas aeruginosa PAO1 using gene replacement
Get PDFEndonuclease I is an enzyme encoded by the endA gene. This nuclease degrades double stranded DNA. Many Escherichia coli common laboratory strains contain a mutation in the endA gene that inactivates the DNA-specific endonuclease I. A mutation in this gene greatly increases plasmid DNA yields in such E. coli strains as well as improves the quality of DNA that is isolated. The purpose of this research is to create an endA mutant strain in Pseudomonas aeruginosa PAO1 using gene replacement, thereby leading to the development of a useful laboratory Pseudomonas strain for use as a cloning strain. To accomplish this, chromosomal DNA from P. aeruginosa PAO1 was isolated, and the endA gene was then amplified by PCR using specific primers designed to the flanking upstream and downstream sequence of the endA coding region. The resulting amplified 1100 bp DNA fragment containing the endA gene was cloned into pCR2.1. This newly created plasmid was named pCR2.1-endA. In order to create an insertionally inactivated endA gene, a GmR encoding cassette from pPS856 needed to be inserted into the SalI sites of the cloned endA gene. The pCR2.1-endA plasmid was digested using SalI restriction enzyme. A 4500 bp SalI fragment of pCR2.1-endA was isolated and then religated by T4 DNA ligase. The new plasmid created was called pCR2.1-endASalID. This plasmid was digested with SalI, and blunt ends were created with T4 DNA polymerase. Inactivation of the endA gene was accomplished by insertion of a blunt-ended, GmR encoding gene into the blunt-ended SalI site of the endA coding sequence. The resulting recombinant plasmid was called pCR2.1-endASalID(Gm1). A 1700 bp HindIII x PstI DNA fragment from pCR2.1-endASalID(Gm1), containing the insertionally inactivated endA gene, was isolated and cloned into the similarly digested pEX18Ap plasmid.High Honors
Biosystems Engineering Research Review 14
No full textThe Fourteenth Annual Research Review describes the ongoing research programme in Biosystems Engineering at University College Dublin. The research programme covers three focal areas: Food and Process Engineering; Bioresource Systems; and Bioenvironmental Engineering. Each area is divided into sub-areas as outlined in the Table of Contents which also includes the name of the research scholar (in bold); the research supervisor(s); the title of the research; the nature of the research programme; and the research sponsors. It also includes the noting of five awards for presentational excellence at the Fourteenth Annual Biosystems Engineering Research Seminar held in University College Dublin on Wednesday 11th March 2009.Author has checked copyrightIt is published by UCD School of Biosystems Engineering annually and printed by UCD Copi-Print. It is edited by myself and Enda Cummins.TS 20.06.1
Biosystems Engineering Research Review 14
Get PDFThe Fourteenth Annual Research Review describes the ongoing research programme in Biosystems Engineering at University College Dublin. The research programme covers three focal areas: Food and Process Engineering; Bioresource Systems; and Bioenvironmental Engineering. Each area is divided into sub-areas as outlined in the Table of Contents which also includes the name of the research scholar (in bold); the research supervisor(s); the title of the research; the nature of the research programme; and the research sponsors. It also includes the noting of five awards for presentational excellence at the Fourteenth Annual Biosystems Engineering Research Seminar held in University College Dublin on Wednesday 11th March 2009.Author has checked copyrightIt is published by UCD School of Biosystems Engineering annually and printed by UCD Copi-Print. It is edited by myself and Enda Cummins.TS 20.06.1
St. Enda Readers
No full textThis school reader contains one fable, The Old Tree and the Gardener attributed to Aesop (63). This is a difficult enough fable. The version of it here may not help clarify things. Its hint is to call the gardener rather greedy at the start of the last paragraph. He refused to spare the tree because of the fruit it had given or the songs it has provided his wife--but he will spare it for the honey he stands to get from it regularly. One possible point to get from this fable would have been that the right appeal gets a person to see it all positively, since the farmer ends up listing all of the tree's benefits past and present, even though he seems to be sparing it only for one advantage, honey. The binding is coming loose, and the book is in fair condition.This is a hardbound book (hard cover)D. O'Dal
Perlindungan Hukum Terhadap Anak Turut Serta Membantu Melakukan Tindak Pidana Penganiayaan (Studi Putusan Nomor 09/Pid.Sus.A/2016/PN.PKL)
No full textPemidanaan diartikan sebagai suatu upaya untuk menyadarkan para pelaku tindak pidana agar menyesali perbuatannya, dan mengembalikannya menjadi warga masyarakat yang baik, taat pada hukum, menjunjung tinggi nilai-nilai moral sosial dan keagamaan, sehingga tercapai masyarakat yang aman, tertib dan damai. Permasalahan dalam skripsi ini adalah bagaimana penerapan konsep diversi dan restorative justice sebagai upaya perlindungan terhadap anak pelaku tindak pidana, bagaimana bentuk perlindungan hukum bagi anak pelaku tindak pidana penganiayaan, bagaimana perlindungan hukum terhadap anak turut serta membantu melakukan tindak pidana penganiayaan berdasarkan putusan Nomor 09/Pid.Sus.A/2016/PN.PKL
Sifat penelitian yang digunakan adalah deskriptif dan jenis penelitian yang dipergunakan adalah penelitian yuridis normatif, yaitu penelitian yang menggunakan peraturan perundang-undangan sebagai dasar pemecahan permasalahan yang dikemukakan. Data yang dipergunakan adalah data sekunder dan metode pengumpulan data yang digunakan dalam penelitian ini adalah Penelitian kepustakaan (Library Research) dan penelitian lapangan (field research). Analisis data yang digunakan adalah data kualitatif.
Penerapan konsep diversi dan restorative justice sebagai upaya perlindungan terhadap anak pelaku tindak pidana dikembangkan sebagai suatu penyelesaian perkara anak yang memberikan perlindungan hukum terhadap anak tersebut yang tidak didasarkan pada suatu pembalasan lagi namun untuk memulihkan anak pada keadaan semula.. Bentuk perlindungan hukum bagi anak pelaku tindak pidana penganiayaan adalah dengan mengedepankan konsep diversi dan restorative justice yaitu semua yang terlibat dalam kasus pidana tertentu bersama-sama memecahkan masalah tentang bagaimana menangani akibat perbuatan anak dimasa yang akan datang. Perlindungan hukum terhadap anak turut serta membantu melakukan tindak pidana penganiayaan berdasarkan putusan Nomor 09/Pid.Sus.A/ 2016/PN.PKL adalah dengan menjatuhkan pidana pengawasan sesuai dengan konsep keadilan restoratif dan diversi yang untuk menghindari stigmatisasi terhadap anak yang berhadapan dengan hukum dan diharapkan anak dapat kembali ke dalam lingkungan sosial secara wajar.
Berdasarkan kesimpulan disarankan diharapkan adanya peraturan pelaksana terhadap undang-undang ini seperti Peraturan Pemerintah terkait diversi sebelum undang-undang ini pada akhirnya berlaku. Peraturan ini juga harus dikaitkan dengan adat istiadat dan kebiasaan di dalam wilayah tertentu sebagai penghargaan terhadap cara penyelesaian perkara anak di wilayah tersebut.Criminalization is defined as an effort to sensitize the perpetrators of crime to regret their actions, and return them to be good citizens, in accordance with the law, upholding social moral values and relationships, promoting a safe, orderly and peaceful society. The problem in this thesis is the problem of applying the concept of diversion and restorative justice as a protection for children who participate in crime, how is the form of legal protection for children who take acts of abuse, how to protect the law against children who help and assist in acts of abuse in accordance with decision No. 09 / Pid. Sus.A / 2016 / PN.PKL
The type of research used is the type of research and the type of research used is normative juridical research, that is, research that uses laws and regulations as guidelines for solving the problem put forward. The data used are secondary data and data collection methods used in this study are library research (library research) and field research (field research). Analysis of the data used is qualitative data.
Application of the concept of diversion and restorative justice as an effort to protect children supported by cases of children who provide legal protection against these children which is not based on further retaliation to improve children in the original country .. prison persecution is by promoting the concept of diversion and restorative justice is all that is involved in certain cases together solve the problem of how to deal with children's problems in the future. Legal protection against children participates in assisting criminal offenses based on decision No. 09 / Pid.Sus.A / 2016 / PN.PKL is by violating licensing in accordance with the concept of restorative justice and diversion aimed at preventing stigmatization of children in conflict with the law and is expected children can return to the natural social environment.
Based on the conclusions that are expected there are implementing regulations for this law such as Government Regulations related to diversion before this law finally comes into force. This regulation must also be challenged with customs and customs in certain areas as an appreciation of how to deal with the cases of children in the region.105 HalamanSkripsi Sarjan
International business assessment of Renault in India
No full textObjective of this report is to discuss and analyze strategic decisions taken by Renault Group to enter in a foreign automobile market India. It also highlights mode of entry as well challenges Renault faced in establishing itself in Indian market. Author keywords: International management, Renault, Indian automobil
In what extent active management brings more value to its investors than passive management?
No full textThe main purpose of this study is to understand in what extent active management brings more value to investors than passive management. This study attempts to answer the theories stating that passive management performs better on the long term than active management.
Since a few years, financial markets are marked by speculation and mainly the rise of algorithmic trading, also called high-frequency trading.
Sources used in this dissertation come from secondary and primary data. The former were collected in books, journals, academic articles, newspapers, and Internet to constitute the literature review. The primary data were gathered by realising semi-structured interviews of fund managers, using active management methods.
In the first part of this dissertation, the literature review is a critical analysis of the subjects surrounding the study. The author defines and identifies the factors impacting fund managers’ portfolios. The managers’ psychology is seen as one of those factors and is developed with the help of Behavioural Finance.
The second part of the study implement the primary data gathered from the interviews. The information is presented, analysed, and used to answer the research questions, as stated in the introduction.
This qualitative dissertation describes and analyses the factors impacting fund managers and their portfolios. It has for objective to understand why active management is known to not perform better than passive management, and what value could it bring to investors.
This research contributes in helping fund managers to use the right investment strategies and philosophies to earn higher returns, and investors to recognise the interest active management can have by giving them the tools necessary for their choices.
As it is a qualitative study, mathematics and algorithms do not enter the scope of this study. Author keywords: Asset management, behaviour, psychology, finance, market, returns, value investing, indexin
Empowerment effects on consumers' behaviour and attitudes in the outdoor sports market
No full textBecause of consumers wake up and discover of the industrial practices, firms had to find a
new strategy to answer to the new consumers‘ wants. To face their increasing want for ethic
marketing and industry, one of the new strategies is consumers‘ empowerment. We decided to
explore the effects of this strategy on consumers of the outdoor market, a market having a strong
like for ethic and using products with a high level of technique. To explore these effects, we
choose to focus on three attitudinal variables (Attitude, Attachment, and Perceived quality) and
one behavioral variable (purchase intention). Author keywords: Empowerment, co-creation, new product development, innovation, alternative marketin
How effectively do major oil producing companies use derivatives in hedging market-based risk?
No full textThe researcher had established the goal to lighten up the crude oil market from the perspective of oil producing companies using derivatives as a part of their risk management strategies. Firstly, the researcher investigated that derivatives are an effective tool of a risk management during the market volatility, however, there was no empirical evidence found toward their effect on company’s enterprise value. Secondly, it has been established that the crude oil market is currently dominated more by speculators rather than hedgers, however, oil producing companies themselves mostly act as hedgers in the market. Author keywords: Finance, Derivatives, Oil industr
A personal filmic exploration of contemporary Irish-Australian identity
No full textThis thesis consists of two parts: a documentary film and a written exegesis. The film, Secret family recipes, explores a personal experience of migration and documents issues of personal identity within broader family, community, and intercultural contexts. The documentary uses the device of cake baking to provide a narrative spine for the journey of exploration. The filmmaker, Enda Murray, journeys from Sydney back to his birthplace in Ireland in 2007 and helps his elderly mother bake her annual Christmas cake. In the course of this journey, he talks to his mother and peers about their memories of growing up and ponders on his own early family life in Ireland. He then returns to Australia and bakes a cake with his two daughters (ages six and four), using this occasion to reflect on his current family situation. The exegesis provides a background context in Irish-Australian history and culture. It examines the major influences on the author’s work as an artist and draws on a range of literature to critique the production of Secret family recipes against the context of Irish documentary, Irish migrant documentary, and Irish-Australian accented cinema. The exegesis argues that Secret family recipes uses elements of ‘performative documentary’, defined by Bill Nichols as documentary that includes the author as a performing character in the film. It also argues that the documentary uses elements of ‘domestic ethnography’, a term coined by Michael Renov to describe filmmaking that explores the complexity of communal or blood ties between the subject and his or her family. This is a form of supplementary autobiographical practice where the subject constructs self-knowledge through the familial other. This research project proposes a new framework of ‘domestic performativity’ within documentary that combines elements of performative documentary and domestic ethnography. This thesis argues that domestic performativity allows a stylised representation of the subject’s voice and combines elements of documentary and ethnography to produce an enhanced autobiographical product
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