1,721,579 research outputs found
Correct usage of a mutated G+1 splice site and transcript quantitation in lipoprotein lipase-deficient patient
No full textThe consequence on the splice mechanism of a mutation occurring at the donor splice site of intron 1 has been studied. We demonstrate that, in spite of the change at G+1 position, this site is still utilized and can produce correctly spliced transcript. Nevertheless the mRNA is detectable only after an 'in vitro' amplification. A procedure has been developed to reveal and quantify the minute amount present in the patient. The very low mRNA level results in a total lack of enzyme, the cause of the disease. The procedure can be useful in cases of rare transcripts and when the source is limited. Furthermore we analyse the interaction between the splice consensus sequence and the small nuclear RNA, that is the necessary intermediate of the splicing mechanism. We speculate on the reasons why cryptic sites are not utilized and only the authentic site can be used, although significantly destabilized by the mutation
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Le parole dopo la morte: forme e funzioni della retorica funeraria nella tradizione greca e romana
No full textVariations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Lipoproteins and apolipoproteins in human cerebrospinal fluid: A new role of lipoprotein(A) and apolipoprotein(A) in the neurological diseases characterized by bloodcerebrospinal fluid barrier dysfunction
No full textThe barrier between blood and CSF contributes to homeostasis of the CNS and
protects it from potentially harmful substances present in the blood.
Lipoproteins present in the CSF are clearly distinct from their plasma counterparts.
Human CSF lipoproteins contain mainly Apo AI and Apo E, the former deriving mostly
from plasma after crossing the blood–cerebrospinal fluid barrier, the latter being also
produced by CNS. Apo AI and E containing lipoproteins in the brain are key players in
transport and delivery of lipids, cholesterol homeostasis, and are also involved in CNS
remodeling mechanisms. On the other hand, the isoform apo E4 represents the most
important genetic risk factor for sporadic and familial late-onset Alzheimer’s disease and
is involved in brain injury and neurodegenerative diseases.
Apo B containing lipoproteins are not produced by CNS and the characterization of
normal human CSF lipoproteins did not allowed the isolation of low density lipoproteins. which is characterized by a dimensional polymorphism. Lipoprotein(a) is a well known
risk factor for athero-thrombosis. The pathological role of Lipoprotein(a) is strictly
associated with its plasma concentrations and the size of apo(a) isoforms, with inverse
relation. The pathophysiology of Lipoprotein(a) in cardio and cerebrovascular system is
widely studied. Recently, we demonstrated that, in neuroinflammatory and
neurodegenerative disorders, Lipoprotein(a) can cross a dysfunctional blood-CSF barrier
and be found in the CSF.
This chapter focuses on the physiological presence of the lipoproteins in CNS, on
the pathological aspects deriving from their isoforms, and in particular on the anomalous
presence in CSF of Lipoprotein(a
An optimal indirect control of underwater vehicle
No full textThis paper considers a new problem of control related to underwater vehicles. Due to the presence of a free fluid surface and to the vortex shedding of the vehicle lifting surfaces, the mathematical model is represented by an integral-differential equation. A new optimal control technique is investigated for this model, and its results compared with the use of a standard LQR method
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