1,721,107 research outputs found
Il cinema cinese della "melodia principale". Storia, istituti, linguaggi e narrazioni
Full text linkLa presente tesi è dedicata allo studio del cinema cinese di propaganda, con particolare riferimento al cosiddetto “cinema della melodia principale” (zhuxuanlü dianying 主旋律电影), categoria istituita nel 1987 che ha raccolto la tradizione propagandistica del cinema della Cina popolare e che rappresenta uno dei prodotti di maggior successo al botteghino cinese. La “melodia principale” è qui analizzata sotto due punti di vista. In primis, attraverso una prospettiva storica, che evidenzia lo sviluppo del cinema cinese di propaganda, impiegando come espediente la ricostruzione degli istituti che hanno controllato, regolamentato e supportato tale produzione, da fine Ottocento al 2022 – terminus ad quem dell’elaborato. In secondo luogo, si presenta un approfondimento sul “cinema della melodia principale” (1987-2022), nel quadro metodologico degli studi sui media e sul cinema cinese. È quindi proposta una periodizzazione delle trasformazioni di tale tipologia di cinema di propaganda e l’analisi di una selezione ragionata di “film della melodia principale”, basata sull’incontro delle teorie dell’analisi filmica e del racconto cinematografico con gli studi sinologici. L’obiettivo è quello di individuare gli elementi alla base dello storytelling propagandistico in termini di linguaggi e narrazioni, attraverso un approccio sensibile alle specificità linguistiche e culturali cinesi
A single point mutation (Glu85Arg) increases the stability of the thioredoxin from Escherichia coli.
No full textGlu85 in the Escherichia coli thioredoxin, which is localized in the loop between beta4 and beta5, was substituted with the Arg present in the corresponding position in Bacillus acidocaldarius thioredoxin. This suggested that it could play an important role in the structure and thermostability of this protein owing to its involvement in numerous interactions. The effects of the mutation on the biophysical properties were analysed by circular dichroism, spectrofluorimetry and limited proteolysis, supported by molecular dynamics data. As modelling predicted, an increase in stability for E85R due to additional H-bonds between the beta5 and alpha4 regions was observed
The machinery for oxidative protein folding in thermophiles.
No full textDisulfide bonds are required for the stability and function of many proteins. A large number of thiol-disulfide oxidoreductases, belonging to the thioredoxin superfamily, catalyze protein disulfide bond formation in all living cells, from bacteria to humans. The protein disulfide isomerase (PDI) is the eukaryotic factor that catalyzes oxidative protein folding in the endoplasmic reticulum; by contrast, in prokaryotes, a family of disulfide bond (Dsb) proteins have an equivalent outcome in the bacterial periplasm. Recently the results from genome analysis suggested an important role for disulfide bonds in the structural stabilization of intracellular proteins from thermophiles. A specific protein disulfide oxidoreductase (PDO) has a key role in intracellular disulfide shuffling in thermophiles. Here we focus on the structural and functional characterization of PDO correlated with the multifunctional eukaryotic PDI. In addition, we highlight the chimeric nature of the machinery for oxidative protein folding in thermophiles in comparison with the mesophilic bacterial and eukaryal counterparts
The peroxiredoxins as molecular guardians in the oxidative stress in Sulfolobus solfataricus.
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Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Involvement of peroxiredoxin bcp2 in oxidative stress in Sulfolobus solfataricus.
No full textReactive oxygen species (ROS) such as superoxide and hydroxyl radicals, hydrogen peroxide, are potent oxidant capable of damaging all cellular components including DNA, proteins and membrane lipids. In order to protect against the toxicity of ROS, organisms are equipped with an array of defence mechanisms. Among these, peroxiredoxins (Prxs) (1), a family of thiol-specific antioxidant proteins, have received significant attention in the last years. Prxs exert their protective antioxidant role in cells through their peroxidase activity, whereby hydrogen peroxide, peroxynitrite and a wide range of organic hydroperoxides are reduced and detoxified.
These enzymes have been identified in each domain of life: Eucarya (2), Bacteria (3)and Archaea (4). Prxs use a redox-active cysteine to reduce peroxides and can be divided into two groups, the 1-Cys and 2-Cys Prxs, based on the cysteinyl residues involved in the catalysis.
[i]S.[/i] [i]solfataricus[/i], is an aerobic hyperthermophilic Archaea whose genome has been sequenced. The genome analysis shows the presence of four putative Prxs (Bcp1, Bcp2, Bcp3, Bcp4) whose physiological roles in the oxidative stress are underway.
We report the involvement of [i]bcp2[/i] gene in oxidative stress, the characterization of the recombinant protein rSsBcp2, its capability to scavenge H2O2 and to protect DNA against the cleavage caused from thiol-mixed –function oxidation (MFO) system
Computational Analysis of the Thermal Stability in thioredoxins: a Molecular Dynamics approach.
No full textThe knowledge of the relationship between the three-dimensional structure of a protein and its biological and stability is one of the most challenging problem in protein chemistry, since offers the possibility of changing both the specific action of a protein and its stability. In this work, we have approached the problem with studies on a protein family, the thioredoxins, using homology procedures, molecular dynamics simulations in vacuo at 300 K and 500 K and in water solution at 300 K, to determine the relationship between the three-dimensional structure of these proteins and their thermal stability. A comparative analysis, using computational approach, was performed between two thioredoxins with different resistance to temperature. Results obtained using the molecular dynamics techniques and minimization procedures give explanations of the experimental data, underlining that these techniques are able to correlate the increase in protein stabilization with the conformational and structural changes caused by single amino acid replacement. In addition, we report the factors that can be used as a guide in protein engineering and in site-directed mutagenesis to increase or decrease thermal stabilization for this protein family
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