1,720,967 research outputs found
An evaluation of an enzyme immunoassay method for immunoreactive trypsin in dried blood spots
No full textA novel monoclonal antibody based enzyme immunoassay (EIA) method for the measurement of the human cationic trypsinogen (NeoScreen, AGEN Biomedical Ltd., Acacia Ridge, Australia) in dried blood spots for the neonatal screening of cystic fibrosis was evaluated. The calibration standards provided as dried blood spots by AGEN are highly unstable and must be replaced with user prepared materials. Reference values from control individuals were obtained by parametric methods. A preliminary comparison with a polyclonal antibody based RIA method (Trypsik, SORIN Biomedica, Saluggia, Italy) was performed. Regression analysis between the RIA and the EIA methods gave a coefficient of correlation of 0.58 for RIA values < 40 •g/L and of 0.77 for RIA values ≥ 40 •g/L. Average CV of the within-run imprecision for the EIA method was 19.6% and for the RIA method 28.8%. CVs of the between-run imprecision at low, intermediate and high values for the EIA method were 23.7%, 15.8%, 15.6% and for the RIA method 20.6%, 14.4%, 11.2%. The diagnostic accuracy analyzed by a Receiver Operating Characteristics (ROC) curve of the RIA method gave a maximum accuracy of 190.9 while that of a simulated ROC curve for the EIA method was 193.0. We found that the precision and the diagnostic accuracy of the EIA method (AGEN) are equal to or better than those of one of the RIA methods
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Is Surgical treatment of lipoblastoma always necessary?
No full textLipoblastoma is an uncommon, benign mesenchymal tumor with an excellent prognosis despite its potential to local invasion and rapid growth. However, in the literature, a spontaneous resolution has never been reported, and, consequently, the need for a complete surgical excision has never been questioned. The authors report a case of a 2-day-old boy with congenital diffuse lipoblastoma in the left thigh, which forced us to withhold from surgical treatment to avoid the risk of mutilation in a patient so young. The lesion was followed-up by imaging, and a complete spontaneous resolution of the diffuse lipoblastoma was shown by magnetic resonance imaging (MRI) at 1-year follow-up. In the literature, a complete surgical excision is recommended. The results of this case suggest that a "wait and see" approach is justified at least in infants with huge invasive lesions requiring a mutilating excision
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Estesa trombosi dei seni venosi durali in un neonato con deficit di AT III: valutazione dell'esordio con encefalografia transfontanellare, TC ed RM, follow-up evolutivo mediante RM ed encefalografia transfontanellare
No full textPLASMA AND RED BLOOD CELL FATTY ACID OF VERY-LOW-BIRTH-WEIGHT INFANTS FED EXCLUSIVELY WITH EXPRESSED PRETERM HUMAN MILK.
No full textNeonatal screening for cystic fibrosis using blood trypsin with complementary meconium lactase: an advisable strategy for the population of southern Europe
No full textIntroduction: Neonatal screening for cystic fibrosis (CF) is now included in several newborn screening programs, based on the view that early presymptomatic diagnosis and therapy for CF reduces the morbidity and mortality from that disease. However, screening for CF is complicated by a relatively high rate of false-positive and a substantial frequency of false-negative results. To reduce the rates of these aberrant results, we have developed a neonatal screening strategy that combines estimation of immunoreactive blood trypsin (IRT) with the meconium lactase activity assay (LACT) for those blood samples that produce the highest 1% of IRT. Methods: This system was used to screen 157 992 newborns in Northeastern Italy from September 1988 to August 1991. Meconium collected just after birth was smeared on filter paper and dried. Immunoreactive trypsin was measured in dried blood spots collected when the infants were 3-5 days old. If the level of IRT exceeded 30 μg/1, LACT was determined in the dried meconium sample. If LACT exceeded 0.5 U/g, a confirmatory sweat test was performed on the infant (mean age, 22 days). If LACT was negative but the IRT level exceeded 40 μg/1, a repeat filter paper blood specimen was collected for IRT measurement. If the IRT level was > 17 μg/1 in this specimen, sweat testing was performed on the infant (mean age, 40 days). Results: We found an incidence of 1:3510 for CF, with 86% of patients identified by LACT among those diagnosed by IRT screening. The initial IRT cut-off level of 30 μg/1 selected 0.83% of screened infants as positive but the complementary use of LACT reduced the rate of sweat tests or requests forrepeat specimens to 0.31%. No false-negative results were encountered, with the exception of three cases of meconium ileus. Discussion: This screening strategy seems to reduce the frequency of false-positive results, while also reducing the rate of false-negative results in neonatal screening for CF. © 1995
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