1,721,001 research outputs found
Evaluating the efficacy of EFTX-016 ASO in inhibiting the most prevalent lung cancer KRAS mutations
Get PDFAntisense oligonucleotides (ASOs) regulate cellular gene expression by hybridizing to a complementary RNA sequence and subsequently activating the RNase H1 degradation pathway. Recent advances in oligomer chemical modifications have paved the way for next-generation ASOs with much greater stability in vivo, showing great potential for ASOs as a cancer therapeutic. EFTX-016 ASO is a selective and potent ASO sequence currently under development and designed to knockdown mutant KRAS expression for cancer treatment. In this study, we characterize the efficacy of a screen of variously modified EFTX-016 ASOs to downregulate KRAS mRNA in representative cancer cell lines. We found that free-uptake of the pan-KRAS EFTX-016 ASO sequence (ASO16), which simultaneously targets the four most common KRAS mutations (G12C, G12V, G12D, and G13D), much less potently knocks down KRAS mRNA than the G12C mutant-specific EFTX-016 ASO sequence (ASO16-G12C). Further modifying ASO16-G12C with locked nucleic acid(s) (LNAs) significantly reduced G12C mutant KRAS expression in vitro while moderately sparing wild type activity. Future studies aim to uncover the clinical potential of the ASO16-G12C sequence by continuing to improve its KRAS knockdown efficiency in vitro, wild type KRAS sparing capacity, and evaluating its ability to modulate growth in lung tumor microenvironments.Bachelor of Scienc
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Data Management and Sharing Plan for: Tumor Endothelial Cell Regulation of Pro-Metastatic Fibrin Matrices
No full textThe Data Management and Sharing Plan describes the scientific data to be generated and/or used in the research and outlines a strategy for managing and sharing project data
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
TUMOR-HOMING CYTOTOXIC INDUCED NEURAL STEM CELLS FOR THE TREATMENT OF PRIMARY AND METASTATIC NON-SMALL CELL LUNG CANCER
Get PDFLung cancer accounts for more deaths than any other type of cancer. 44% of all brain metastases are due to non-small cell lung cancer (NSCLC), and the median survival for these patients, particularly if leptomeningeal metastases (LMM) are present, is only 3 months. Radiation is frequently used to treat NSCLC LMM, but this treatment alone cannot eradicate LMM. New therapies are desperately needed to treat this deadly disease, both in the primary stage and metastatic stages. Neural stem cells (NSCs) offer a useful method of drug delivery in the treatment of NSCLC because of their inherent tumoritropic properties. NSCs can be genetically engineered to secrete cytotoxic proteins to deliver a potent payload directly to tumors. In order to facilitate a readily available supply of autologous, tumor-homing cells, human induced neural stem cells (hiNSCs) can be transdifferentiated from an individual patient’s fibroblasts. A second generation of hiNSCs called hiNeuroS using a cell sphere-based culture system has shown significant improvement in migratory capacity compared to first-generation hiNSCs. This dissertation investigates the use of intravenous (IV) first-generation hiNSCs to track down and treat primary NSCLC and intracerebroventricular (ICV) second-generation hiNeuroSs to treat NSCLC LMM when combined with radiation.Doctor of Philosoph
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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