112,190 research outputs found
Studies on the Effect of Nuclear-penetrating Anti-dsDNA Autoantibody IgG and Recombinant VH Domain in Lupus Nephritis-associated Kidney Cells
No full textThe aim of this study is to investigate the pathological impacts of the anti-dsDNA antibodies, that are basically causative factors in autoimmune diseases. The anti-dsDNA autoantibody 2C10 IgG and its recombinant fragment 2C10 VH domain were tested in the normal murine kidney mesangial cells (MES) and human cervical cancer cell (HeLa) to know the pathogenic aspect of these antibodies. It was observed that the 2C10 IgG and 2C10 VH domain penetrated into the target cells and reduced their cell proliferation and the penetrated autoantibodies simultaneously activated the signal molecules ERK1/2 and p38. Further observation revealed that 2C10 IgG and 2C10 VH persistently activated RSK-1 and upregulated BCL-2 expression. In mesangial cell line, the 2C10 IgG and 2C10 VH stimulated the transcription of pro-inflammatory genes TNF-α, IL-6 and IL-1β through ATF-2 activation enormously, but the same pattern was not observed in HeLa cells. The activation of these signal molecules was linked to the production of pro-inflammatory cytokines, because inhibition of the molecules suppressed the cytokine production. Collectively, the cell-and nuclear-penetrating autoantibodies 2C10 IgG and 2C10 VH seem to play a pathogenic role in mesangial cells through activation of p38, MAPKAPKK2, RSK-1, BCL-2 and ATF-2 and consequent production of pro-inflammatory cytokines.I. INTRODUCTION 1
II. MATERIALS AND METHODS 4
1. Cell Lines 4
2. Antids DNA Monoclonal Antibodies 4
3. Cell signaling Antibodies and Inhibitors 5
4. Cytokine Primers 5
5. Cell proliferation assay 6
6. Confocal Microscopy 6
7. Flow Cytometry for cell cycle analysis 7
8. Western Blotting 7
9. Quantitative realtime PCR 8
10. Statistic analysis 8
III RESULTS 9
1. AntidsDNA autoantibody (autoAb) 2C10 IgG and 2C10 VH have cell penetrating property 9
2. Penetrating autoAbs 2C10 IgG and 2C10 VH inhibit the cell survival in various cell lines 9
3. 2C10 IgG and 2C10 VH fragment activated the ERK1/2 and p38 molecules 15
4. 2C10 IgG and 2C10 VH increased the mRNA level of TNFα, IL6, IL1β in MES cells 20
IV. DISCUSSION 24
V. REFERENCES 28Maste
Tumor-associated Collagen Signatures: An Insight
No full textABSTRACT
Tumor microenvironment has a diverse capability to induce both beneficial and adverse consequences for tumorigenesis. It is a multifactorial process induced by the imbalance in the tumor cells and extracellular matrix (ECM). Collagen, the main component of ECM, is traditionally regarded as a passive barrier to resist tumor cell invasion. In recent years, collagen is marked to have its pivotal role to initiate and promote tumor progression. Remodeling of collagen has been appreciated in various benign and malignant tumors. These alterations can be identified and demonstrated as tumor-associated collagen signatures that can be demonstrated using second harmonic generation imaging. Recognition of these characteristic changes in the organization of collagen fiber may potentially serve as an early diagnostic marker in various pathological processes, such as hyperplastic, dysplastic, and cancerous tissues. This review focuses on the physiological and pathological orientation of collagen fibers in relation to epithelium that acts as an image-based biomarker.
How to cite this article
Pavithra V, Sowmya SV, Rao RS, Patil S, Augustine D, Haragannavar VC, Nambiar S. Tumorassociated Collagen Signatures: An Insight. World J Dent 2017;8(3):224-230.
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author-bios-SRD-19-0063.R1 – Supplemental material for The Network Structure of Police Misconduct
No full textSupplemental material, author-bios-SRD-19-0063.R1 for The Network Structure of Police Misconduct by George Wood, Daria Roithmayr and Andrew V. Papachristos in Socius</p
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Využití sociálních médií v B2B prodeji
No full textTato diplomová práce se zabývá tím, jak mohou B2B obchodníci využívat sociální média v prodeji. Na základě systematické rešerše literatury, autor zjistil, že akademici, zkoumající danou problematiku, navrhují další výzkum, a to: v kterých konkrétních krocích se dají využít sociální média v prodeji (Salo, 2017). Autor se na základě toho rozhodl zjistit, jaké sociální sítě, různé technologie a pluginy se dají využít v B2B prodeji - tzv. social sellingu. Social selling se v této práci týká primárně procesu akvizice a okrajově péčí o stávající zákazníky. Autor si vybral kvalitativní průzkum pomocí 10 hloubkových polo-strukturovaných rozhovorů, aby odhalil jak, která sociální média to jsou, tak i motivaci prodejců, proč tato média používat/nepoužívat. Aby autor dodržel správnost vyhodnocení výsledků, data byla analyzována pomocí Tématické analýzy, která v této studii vykrystalizovala 2 hlavní strategické přístupy v social sellingu. Tyto přístupy (tzv. Push a Pull strategie) obsahují praktické příklady a konkrétní aktivity, které mohou prodejci využívat v každodenní praxi. Tyto výsledky jsou prezentovány s důrazem na praktičnost a jednoduchost implementace. Tvoří proto hlavní přínos autorovo výzkumu. V poslední části autor zmiňuje výzvy a manažerská doporučení, které mohou obchodníci využít v každodenním pracovním životě.This diploma thesis focuses on social media usage in B2B sales. Based on the systematic literature review conducted by the author, he has found out that recent researchers (Salo, 2017) suggest further research in the area of how and in which sales phase should various social networking sites, technologies and plugins used. To further fill this research gap, author decided to identify these social media and their usage among B2B salespeople in the so-called social selling process. The social selling process in this thesis applies mainly to acquiring new prospects and tangentially to taking care of existing clients (follow-up step). Author has chosen a qualitative research method via conducting 10 in-depth semi-structured interviews to reveal these instruments as well as motivation of a sales person on why to use social media in the selling process. The collected data was analyzed using Thematic analysis to ensure the right procedure and to identify main themes which crystalized into 2 main strategic approaches in social selling. These approaches (Push and Pull) include practical examples of concrete activities which sales people can use in their daily jobs and are presented with focus on practicality and ease of implementation. These also form the main contribution of author`s research. In the last part, author mentions challenges in social selling and recommended managerial implications for salesforce
TeX v jednoduchém unixovém prostředí
No full textsummary:Při ladění TeXového dokumentu potřebujeme mnohokrát opakovaně pouštět TeX, podívat se, jak dopadl výsledek v prohlížeči DVI nebo PDF souboru, mrknout na výpis TeXu na terminálu, podívat se případně do logu a celou činnost opakovat. V tomto článku je ukázáno, jak tuto práci dělá autor článku. Proces "editor-TeX-kuk" je zde podporován jednoduchými unixovými nástroji: bashovým skriptem texloop, který si autor pro tyto účely vytvořil, dále terminálem Xterm a jednoduchým editorem, který umí navázat na klávesovou zkratku spuštění příkazu v systému. Čtenář se zde může inspirovat a přizpůsobit tyto nástroje svým vlastním potřebám. V článku je popsána funkce skriptu texloop, dále je neformálně rozveden dlouholetý vývoj autorova vztahu k textovým editorům a konečně je zde uvedena konfigurace terminálu Xterm, aby vyhovoval českému prostředí jak v kódování ISO-8859-2, tak v kódování UTF-8. Pro kódování UTF-8 si v závěru článku vygenerujeme TeXový formát csplain.summary:By debugging a TeX document it is necessary many times repeatedly to run TeX, to look for the result in DVI or PDF file, to gander the TeX output on the terminal, or eventually to have a look in the log-file, and all that action to repeat. In the paper it is show, how this work is made by author. The process '‘'editor-TeX-look' is supported by simple Unix tools: bash script texloop, created by author for these purposes, Xterm terminal and a simple editor, which is able to link to the shortcut key the activation of a system command. The reader could be inspired with the solution and to adapt these tools to his/her own needs. In the paper the function of the texloop script is described, and further the longstanding evolution of the author's relation to text editors is informal elaborated and finally a configuration of Xterm terminal, suitable for the czech environment with both ISO-8859-2 and UTF-8 encoding is introduced. For UTF-8 encoding the TeX format csplain is generated at the end of the paper
Investigation of three recent ATLAS V Centaur upper stage fragmentation events
No full textThree Centaur upper stage fragmentation events – 2009-047B, 2014-055B, 2018-079B – that took place in 2018and 2019 are studied. Ephemerides and Two-Line Ele-ments (TLEs) of the fragments, from three different datasources, are propagated to calculate the breakup epoch.Additionally, techniques developed by Tan and Reynolds[22] to obtain Gabbard plots, velocity & angular pertur-bations of the fragments, and event intensities, are ap-plied to the Centaur upper stage events. From the results,hypotheses regarding the causes of the events are postu-lated. The Centaur fragmentations stray from breakuppatterns exhibited by ’typical’ upper stage fragmenta-tions such as those pertaining to the historic explosiveDelta upper stage events. Only one of the three events,2018-079B, appears to have fragmented due to the com-bustion of leftover propellant. 2009-047B, on the otherhand, likely endured a structural failure – inferred fromclustered fragments and low fragment spreading speeds.A torus-shaped fragment distribution is observed for the2014-055B event, suggesting a collision with a smallpiece of debris may have taken place
Analýza perspektivy vedoucích prodeje na řízení pojišťovacích agentů v etnicky různorodých zastoupeních v Organizaci G, Malajsie.
No full textCílem této práce je odhalit klíčové faktory a výzvy spojené s řízením etnicky různorodého pojišťovacího zastoupení v oboru životního pojištění v Malajsii. Autor si klade za cíl zkoumat pohled vedoucích prodeje v Organizaci G v Malajsii na jejich způsob řízení agentů. Kombinované kvantitativní a kvalitativní metody budou použity prostřednictvím průzkumu a rozhovorů se 79 agenty a 12 vedoucími prodeje. Odpovědi budou analyzovány za účelem porozumění výzvám, kterým čelí vedoucí prodeje při řízení etnicky různorodého zastoupení, a zkoumat potenciální řešení. Autor navrhuje iniciativy a metody k posílení stylů vedení a zlepšení procesů pro udržení úspěšného zastoupení v kontextu etnické rozmanitosti.The goal of this thesis is to uncover the key factors and challenges in managing an ethnically diverse insurance agency in Malaysia's life insurance industry. The author aims to examine the sales managers' perspective in Organisation G, Malaysia, regarding their expression of managing their agents. Quantitative and qualitative methods will be employed through surveys and interviews among 79 agents and 12 sales managers. The responses will be analysed to understand the challenges sales managers face in managing a multi-ethnic agency and explore potential solutions. The author proposes initiatives and methods to enhance leadership styles and improve the processes for maintaining a successful agency in the context of multi-ethnicity
AUTHOR BOOK
No full textVytvořil jsem autorskou knihu o čtyřiceti stránkách s ilustracemi a minimem textu. Kniha je černobílá, dominuje v ní tušová kresba štětcem a perem. Kromě té je v knize ale i text, který slouží jako poetické doplnění ilustrací.
Obsah knihy se zaměřuje na úzký kruh přátel a zároveň vypovídá o autorovi jak vybranými motivy, situacemi tak linkou.
Vyznění knihy je spíše pocitové, má zanechat dojem, ne popisně vysvětlovat.ObhájenoI have created author book that has 40 pages with ilustrations and minimum of text. The book is black and white and contains ink drawings. There is also text poetically coresponding with pictures
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