262,290 research outputs found
Specificity of PluR and PauR towards different signaling molecules.
No full text<p>PluR (<b>A</b>) senses its cognate signaling molecule PPYD, whereas unrelated signaling molecules, like C8-HSL or DAR, CHDA, CHDB and IPS, are not sensed. To test the specificity of PluR the reporter system pBAD24-His-<i>pluR</i> and pBBR1-<i>pcfA</i><sub><i>P</i>.<i>l</i>.</sub>-<i>lux</i> was used. Similarly, PauR (<b>B</b>) specifically senses its native signaling molecules with the highest specificity towards DAR compared to the DAR-precursors, CHDA, CHDB and IPS. The PauR-specific reporter plasmid system composed of pBAD24-His-<i>pauR</i> and pBBR1-<i>pcfA</i><sub><i>P</i>.<i>a</i>.</sub>-<i>lux</i> was used. Cells harboring the promoter-less reporter plasmid in combination with each PluR and PauR did not exhibit significant <i>pcfA</i> promoter activity. Furthermore, cells harboring the empty pBAD24 plasmid, and therefore no <i>pluR</i> or <i>pauR</i>, with the respective reporter plasmid as well did not exhibit significant <i>pcfA</i> promoter activity. RLUs are shown for 2 h after addition of the depicted signaling molecule. Reference line was set to 370 RLUs to underline the background of the system. RLU, relative light units. (<b>C</b>) Comparison of the structures of the signaling molecules used in this study.</p
The TYDQYI-motif in the SBD of PauR is essential for the overall functionality of the receptor and DAR-sensing.
No full text<p>The most drastic effects on DAR-sensing were gained with the replacement of S38A, T62A, Y66A, D75A and D75N in the SBD of PauR and a decreased effect on DAR-sensing were gained with the replacement of Y90C and I113S in PauR (lower right quadrant). The PauR derivatives Y40A, Y40F, D75E and Q76A dramatically influenced the structure of PauR and decrease its ability to bind and activate <i>pcfA</i><sub>P.a.</sub> promoter (lower left quadrant). The activity of <i>pcfA</i><sub>P.a.</sub> promoter was measured via luminescence as read-out and pictured values were taken 2 h after addition of 0.1% arabinose (lower axis) or 3.5 nM DAR (left axis) and compared to PauR wild type, which values were set to 100% (upper right quadrant). To evaluate the different derivatives, a cut-off of 70% was set for each value. RLU (relative light units) values for all PauR derivatives and PauR wild type are depicted in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0124093#pone.0124093.s005" target="_blank">S4 Table</a>.</p
The conserved motifs in the SBD of PluR and PauR are essential but not sufficient for ligand-binding specificity.
No full text<p>(<b>A</b>) Stepwise replacement of the non-conserved amino acids in PluR to the conserved WYDPWG-motif of AHL-sensors effects the conformation and decreases its ability to activate <i>pcfA</i><sub>P.l.</sub> promoter compared to PluR wild type (wt). The quadruple replacement of PluR-T62W/Q76P/C90W/S115G effects most dramatically the conformation compared to PluR wild type. (<b>B</b>) Stepwise replacement of the non-conserved amino acids of PluR decreases its ability to sense its native signaling molecule PPYD, however C8-HSL-sensing could not be gained. <b>(C)</b> Replacement of Y90C and I113S in PauR decreased the ability of PauR-Y90C/I113S to activate <i>pcfA</i><sub>P.a.</sub> promoter activity approximately to 50% compared to PauR wild type. (<b>D</b>) DAR-sensing was decreased approximately about 70% in the PauR-Y90C/I113S derivative compared to PauR wild type (wt), however, PPYD-sensing could not gained. (<b>A</b>) and (<b>C</b>): RLUs are shown 2 h after induction and value of PluR or PauR wild type was set to 100% and compared to the respective derivatives. (<b>B</b>) and (<b>D</b>): The RLU values are depicted 2 h after the addition of the distinct signaling molecules, either 3.5 nM PPYD, 3.5 nM DAR or 100 nM C8-HSL. Reference line was set to 370 RLUs to compare better with <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0124093#pone.0124093.g002" target="_blank">Fig 2</a>.</p
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Cash is not enough: child discipline, humanitarian assistance, and Syrian refugees in Lebanon
No full textSubmission original under an indefinite embargo labeled 'Open Access'. The submission was exported from vireo on 2022-11-15 without embargo termsThe student, Brayden Paur, accepted the attached license on 2022-07-15 at 16:53.The student, Brayden Paur, submitted this Thesis for approval on 2022-07-15 at 17:00.This Thesis was approved for publication on 2022-07-19 at 12:07.DSpace SAF Submission Ingestion Package generated from Vireo submission #18347 on 2022-11-15 at 18:21:20The effects of cash programs in humanitarian settings on caregiver’s use of child discipline has not been widely studied. Violent discipline on children can have long adverse effects lasting into adulthood. Poverty and food insecurity are both correlated with violent discipline and present a potential pathway for cash transfers to affect this outcome. I use three years of data from 2018, 2019, and 2020 for Syrian refugees in Lebanon to assess the impact of two large cash programs: Multi-Purpose Cash and Cash for Food. Assistance for each program is distributed in a bottom up approach based on households’ proxy mean test (PMT) score, a proxy for vulnerability, that allows for the use of a regression discontinuity design (RDD). Findings suggest that neither program affects parents’ use of discipline. In this context, large monthly cash transfers, each worth approximately 50% of monthly household expenditure on average, are not enough to improve violent discipline outcomes. To address violent discipline, other research has found that cash plus programs, which in addition to cash transfers, include programming to instruct caregivers how to discipline children in a non-violent manner effectively decreases use of violent discipline
Bilberry juice modulates plasma concentration of NF-kappaB related inflammatory markers in subjects at increased risk of CVD
No full textProtein sequence comparison of QS LuxR family members and the non-AHL sensors PluR and PauR.
No full text<p>(<b>A</b>) Modular domain structure of LuxR-type regulators, with a N-terminal signal-binding domain (SBD) and a C-terminal DNA-binding domain (DBD) [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0124093#pone.0124093.ref008" target="_blank">8</a>], containing the helix-turn-helix "HTH LUXR" motif (SMART00421) [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0124093#pone.0124093.ref025" target="_blank">25</a>]. (<b>B</b>) Comparison of the protein sequence identity of PluR from <i>P</i>. <i>luminescens</i>, PauR from <i>P</i>. <i>asymbiotica</i> and TraR from <i>A</i>. <i>tumefaciens</i>. The identity was compared either of the full-length protein sequence, only the signal-binding domain (SBD) and only the DNA-binding domain (DBD). To calculate identity of the protein sequences the LALIGN software from SIB (Swiss Institute of Bioinformatics) was used [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0124093#pone.0124093.ref026" target="_blank">26</a>]. (<b>C</b>) Sequence alignment of the protein sequences of PauR from <i>P</i>. <i>asymbiotica</i> (<i>P</i>.<i>a</i>.), PluR from <i>P</i>. <i>luminescens</i> (<i>P</i>.<i>l</i>.), QscR from <i>Pseudomonas aeruginosa</i> (<i>P</i>.<i>a</i>.), SdiA from <i>Escherichia coli</i> (<i>E</i>.<i>c</i>.), TraR from <i>Agrobacterium tumefaciens</i> (<i>A</i>.<i>t</i>.) and LuxR from <i>Vibrio fischeri</i> (<i>V</i>.<i>f</i>.). The SBD is depicted with a blue bar and the DBD with a green bar. Within the SBD the six conserved amino acids, displaying the WYDPWG-motif of AHL-sensors, are marked with red asterisks and the three conserved amino acids in the DBD are marked with blue asterisks. Amino acids with a consensus of 60–100% are shown, positions with a lower coverage are marked with a dot. The RasMol colouring of the amino acids and the alignment was generated with CLC Mainworkbench 7 (CLC Bio Qiagen, Hilden, Germany).</p
Protecting Animals 36: Author Witi Ihimaera
No full textIn this very special episode of Knowing Animals I am joined by beloved New Zealand author Witi Ihimaera. Witi has written many books featuring nonhuman animals. He offers us a non-colonial lens through which to think about the human/nonhuman relationship
Author Under Sail The Imagination of Jack London, 1893-1902
No full textIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Intro -- Title Page -- Copyright Page -- Dedication -- Contents -- Acknowledgments -- Introduction -- 1. Spirit Truth -- 2. From Absorption to Theatricality and Back Again -- 3. "I Will Build a New Present" -- 4. Sons as Authors -- 5. Fathers as Publishers -- 6. The Daughter as Author -- 7. Lovers as Authors -- 8. At Sea with the Family -- 9. Yellow News, Yellow Stories -- 10. The Return Home -- Notes -- Bibliography -- Index -- About Jay WilliamsIn Author Under Sail, Jay Williams offers the first complete literary biography of Jack London as a professional writer engaged in the labor of writing. It examines the authorial imagination in London's work, the use of imagination in both his fiction and nonfiction, and the ways he defined imagination in the creative process in his business dealings with his publishers, editors, and agents. In this first volume of a two-volume biography, Williams traverses the years 1893 to 1902, from London's "Story of a Typhoon" to The People of the Abyss. The Jack London who emerges in the pages of Author Under Sail is a writer whose partnership with publishers, most notably his productive alliance with George Brett of Macmillan, was one of the most formative in American literary history. London pioneered many author models during the heyday of realism and naturalism, blurring the boundaries of these popular genres by focusing on absorption and theatricality and the representation of the seen and unseen. London created an impassioned, sincere, and extremely personal realism unlike that of other American writers of the time. Author Under Sail is a literary tour de force that reveals the full range of London as writer, creative citizen, and entrepreneur at the same time it sheds light on the maverick side of machine-age literature.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
Author in Essay by I. A. Goncharov “Pepiniere”
No full textFeatures of the embodiment of the author’s position in the essay by I. A. Goncharov “Pepiniere” are considered. The relevance of the study is due to the poorly studied poetics of this work. A review of the scientific literature on relevant topics is performed. Methodological and theoretical definitions are given. The scientific novelty of the article is in the fact that for the first time attention is paid to artistic techniques that allow to identify the author's position in the specified literary text. The author of the article grounds her opinion from the fact that, despite the dominance of the subjective point of view, other characters’ views stand out in the work. It is concluded in the study that the text of the work represents a biographical author and author-creator. It was established that the position of the author-creator is expressed through the title, epigraphs, which are quotes, as well as through different points of view, including the author-character, the author-narrator, the characters of the work. The author of the article dwells in detail on different ways of expressing the points of view of the author-character and the author-narrator. It is proved that the point of view of the author-character and the author-narrator can intersect, they are interchanged. The author's development of the term comic “point of view” is presented in the article
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