1,721,004 research outputs found
Zymographic Analysis of Matrix Metalloproteinases (MMP-2 and MMP-9) in Cerebrospinal Fluid and Sera from Patients with Multiple Sclerosis
No full textThe gelatinase MMP-9like is involved in regulation of LPS inflammatory response in Ciona robusta
No full textMatrix metalloproteinases (MMPs) are a family of endopeptidases collectively able to degrade the components of
the extracellular matrix (ECM), with important roles in many biological processes, such as embryogenesis,
normal tissue remodelling, angiogenesis and wound healing. New views on the function of MMPs reveal that
they regulate inflammatory response and therefore might represent an early step in the evolution of the immune
system. MMPs can affect the activity of cytokines involved in inflammation including TGF-β and TNF-α. MMPs
are widely distributed in all kingdoms of life and have likely evolved from a single-domain protein which
underwent successive rounds of duplications. In this study, we focused on the Ciona robusta (formerly known as
Ciona intestinalis) MMP gelatinase homologue. Gene organization, phylogenetic analysis and 3D modeling supported
the closest correlation of C. robusta gelatinase with the human MMP-9. Real-time PCR analysis and
zymographic assay showed a prompt expression induced by LPS inoculation and an upregulation of enzymatic
activity. Furthermore, we showed that before of the well-known increase of TGF-β and TNF-α levels, a MMP-
9like boost occurred, suggesting a possible involvement of MMP-9like in regulating inflammatory response in C.
robusta
Prognostic and functional significant of mmp2 and mmp9 in breast cancer unveiled by proteomic analysis
No full text
DISCOVERY OF POTENTIAL BIOMARKERS IN MULTIPLE SCLEROSIS VIA CEREBROSPINAL FLUID PROTEOMIC PROFILING
No full textDISCOVERY OF POTENTIAL BIOMARKERS IN MULTIPLE SCLEROSIS VIA CEREBROSPINAL FLUID PROTEOMIC PROFILIN
Fluorescent naphthalimide-imidazolium hydrogels for biomedical applications
Full text linkBioimaging and in vivo imaging are cornerstone technologies in support of biomedical diagnosis. However, in some cases imaging methods have increased cancer risks for patients. Moreover, the most widely used diagnostic medical
imaging technique, X-ray imaging, is the largest man-made source of radiation exposure to the general population. Thus, the research of new efficient and less invasive materials for imaging is quite urgent. Supramolecular hydrogels have recently proved to be promising biological carriers to load versatile bioimaging agents for in vitro or in vivo bioimaging, thanks
to the ability to undergo reversible swelling and gel–sol transition in response to various physiological stimuli. In addition, the biodegradability and biocompatibility allowed the use of supramolecular gels also for cancer diagnosis, as they can be
facilely endocytosed into cells [1]. Remembering the good biological response of some imidazolium derived hydrogels [2], fluorescent imidazolium organic salts, that should own the double function of gelator and bioimaging agent, have been synthesized. New fluorescent hydrogels with interesting physico-chemical properties (rheology, gel-sol temperature transition and optical properties) have been tested for anti-proliferative activity, in vitro bioimaging on cancer cells and controlled release of gelator in physiological medium. Results evidence how these hydrogels can be potentially investigated as new theranostic media for anticancer researc
New Synthetic Nitro-Pyrrolomycins as Promising Antibacterial and Anticancer Agents
Full text linkPyrrolomycins (PMs) are polyhalogenated antibiotics known as powerful biologically active compounds, yet featuring high cytotoxicity. The present study reports the antibacterial and antitumoral properties of new chemically synthesized PMs, where the three positions of the pyrrolic nucleus were replaced by nitro groups, aiming to reduce their cytotoxicity while maintaining or even enhancing the biological activity. Indeed, the presence of the nitro substituent in diverse positions of the pyrrole determined an improvement of the minimal bactericidal concentration (MBC) against Gram-positive (i.e., Staphylococcus aureus) or -negative (i.e., Pseudomonas aeruginosa) pathogen strains as compared to the natural PM-C. Moreover, some new nitro-PMs were as active as or more than PM-C in inhibiting the proliferation of colon (HCT116) and breast (MCF 7) cancer cell lines and were less toxic towards normal epithelial (hTERT RPE-1) cells. Altogether, our findings contribute to increase the knowledge of the mode of action of these promising molecules and provide a basis for their rationale chemical or biological manipulation
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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