1,720,983 research outputs found
Low incidence of complications due to local anaesthesia for fiberoptic bronchoscopy (FB) by tracheal injection (TI)
No full textChronic treatment with a synthetic cannabinoid CP-55,940 alters G-protein expression in the rat central nervous system.. 1997 Mar;44(2):191-7
No full textMorphine withdrawal syndrome and G protein expression: a study of the time course in the rat central nervous system.. 1995 Nov 1;7(11):2334-40
No full textChronic treatment with a synthetic cannabinoid CP-55,940 alters G-protein expression in the rat central nervous system
No full textProlonged exposure of rats to the synthetic cannabinoid receptor ligand, CP-55,940 (0.4 mg/kg, i.p. for 11 days), induced tolerance to analgesia, to the reduction in spontaneous locomotor activity and the incidence of splayed hind limbs. One hour after the last injection on day 11, the rats were killed and in situ hybridization was used to investigate the effect of treatment on G-protein α-subunit expression throughout the brain. Chronic cannabinoid exposure markedly reduced Gα(s), Gα1 and Gα0 mRNA levels. The message for the αs-subunit was decreased in all the brain areas containing the basal autoradiographic signal; the decrease ranging from 25% in the thalamus to 45% in the mesencephalon. Also the basal Gal expression was reduced in tolerant rats showing the greatest decrease in the forebrain (63%) in the cerebellum (58%) and in the mesencephalon (38%). The reduction in Gα0 expression (25%) was more localized, being present only in the rostral portion of the brain (cortex, striatum and olfactory area). The alterations in α-subunits gene expression were not followed by any change in the amount of proteins. Our results indicate that, besides the receptor modification, alteration to the G-protein expression could be a molecular event associated with the development of cannnabinoid tolerance
Central pertussis toxin abolishes the intestinal inhibition induced by intracerebroventricular morphine
No full textRegulation of immune functions in rat splenocytes after acute and chronic in vivo treatment with CP-55,940, a synthetic cannabinoid compound.
No full textMorphine Withdrawal Syndrome and G Protein Expression: a Study of the Time Course in the Rat Central Nervous System
No full textWe followed the changes in G protein α subunit expression and levels throughout the brain during the naltrexone‐precipitated withdrawal syndrome in morphine‐dependent rats. Intraperitoneally injected naltrexone (10 mg/kg) in rats made tolerant to morphine resulted in sustained withdrawal. Additional naltrexone doses 6, 24 and 72 h later still induced a significant abstinence syndrome. At the fifth naltrexone injection (8 days later) counted signs were completely resolved but checked ones were not. Besides the behavioural modifications, opiate withdrawal affected G protein expression in the central nervous system. In situ hybridization showed that Gαs and Gαo mRNA, whose levels are increased in tolerance, changed further during opiate withdrawal. Specifically, the αs mRNA in the hypothalamus was reduced after the first naltrexone injection and reached the control level with subsequent doses. However, αo mRNA expression in the olfactory system remained elevated after repeated naltrexone injections, declining to the control value only after the fifth dose. The amounts of Gαs and Gαo protein closely followed the time course of mRNA. The relationship between behavioural and biochemical parameters is discussed, together with the regional selectivity of the modifications. Copyright © 1995, Wiley Blackwell. All rights reserve
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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