1,585 research outputs found

    Stable Isotope Probing and Raman Spectroscopy for Monitoring Carbon Flow in a Food Chain and Revealing Metabolic Pathway

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    Accurately measuring carbon flows is a challenge for understanding processes such as diverse intracellular metabolic pathways and predator-prey interactions. Combined with stable isotope probing (SIP), single-cell Raman spectroscopy was demonstrated for the first time to link the food chain from carbon substrate to bacterial prey up to predators at the single-cell level in a quantitative and nondestructive manner. Escherichia coli OP50 with different C-13 content, which were grown in a mixture of C-12- and fully carbon-labeled C-13-glucose (99%) as a sole carbon source, were fed to the nematode. The C-13 signal in Caenorhabditis elegans was proportional to the C-13 content in E. coli. Two Raman spectral biomarkers (Raman bands for phenylalanine at 1001 cm(-1) and thymine at 747 cm(-1) Raman bands), were used to quantify the C-13 content in E. coli and C. elegans over a range of 1.1-99%. The phenylalanine Raman band was a suitable biomarker for prokaryotic cells and thymine Raman band for eukaryotic cells. A biochemical mechanism accounting for the Raman red shifts of phenylalanine and thymine in response to C-13-labeling is proposed in this study and is supported by quantum chemical calculation. This study offers new insights of carbon flow via the food chain and provides a research tool for microbial ecology and investigation of biochemical pathways

    Annual report of the New Jersey State Museum, 1908, including a report of the birds of New Jersey: their nests and eggs: And notes on New Jersey fishes, amphibians and reptiles

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    In presenting its annual report for 1908, the New Jersey State Museum is carrying out the plan laid out by the Commission and the Curator, to make the Museum reports on the same plan as the Museum was established- purely educational. This report treats of the "Birds of New Jersey, their Nests and Eggs." The Museum feels that no subject could be taken for the report that would be more interesting and beneficial in the cause of education than this. The subject-matter of "The Birds, their Nests and Eggs" has been prepared by Mr. Witmer Stone, Curator of the Academy of Natural Sciences of Philadelphia, a gentleman having a national reputation as a naturalist. To the Bird Report are added some notes of the New Jersey Fishes, Amphibians and Reptiles by Henry W. Fowler

    Design of annulene-within-an-annulene systems by the altanisation approach. A study of altan-[n]annulenes

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    The altanisation strategy, devised to design molecules with large and paratropic perimeter circulations, is applied to the family of [n]annulenes to give, altan-[n]annulenes, i.e. [n,5]coronenes. Analytical expressions are obtained for the eigenvalues of the Hückel Hamiltonian for altan-[n]annulenes, and used in conjunction with selection rules derived from the ipsocentric approach to predict patterns of global ring current in these systems. Density-functional calculations performed on seven altan-[n]annulenes, three neutral and four charged, give current-density maps in essential agreement with the predictions obtained at the unperturbed Hückel level. All but one of the systems show patterns with the tropicities expected for isolated annulenes, in line with the altanisation concept. The apparent exception is altan-[11]annulene−, the only singlet system with a well defined open-shell character in the studied set. The key role of open-shell character can be accommodated by appropriate choice of the occupation numbers of the initial Hückel molecular orbitals, where the anion altan-[11]annulene− is considered as an [11]annulene inside the [22]annulene anion

    Enduring Liberalism: American Political Thought Since the 1960s

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    Robert Booth Fowler is professor of political science at the University of Wisconsin-Madison and the author of The Greening of Protestant Thought; Religion and Politics in America (with Allen Hertzke); and The Dance With Community: The Contemporary Debate in American Political Thought. With a New Foreword by Jefferson Decker.This Kansas Open Books title is funded by a grant from the National Endowment for the Humanities and the Andrew W. Mellon Foundation Humanities Open Book Program.Has the United States become more pluribus than unum? In terms of the nation's political beliefs, Robert Booth Fowler answers both yes and no. While his study affirms significant diversity among an elite cadre of public intellectuals, it vigorously denies it in a general public that collectively adheres to the same set of liberal core values. Enduring Liberalism pursues two objectives. One, it explores the political thought of public intellectuals and the general public since the 1960s. Two, it assesses contemporary and classic interpretations of American political thought in light of the study's findings. Fowler interprets the writings of public intellectuals like Robert Bellah, Jean Bethke Elshtain, Michael Walzer, William Bennett, Seymour Martin Lipset, William Galston, and others, as well as survey data of American political attitudes, to spotlight this oft-ignored divide between citizens and high-profile commentators, whose contentious debates are mistakenly assumed to reflect countrywide rifts. Fowler's argument is straightforward, but the interpretation is controversial. He recounts how the consensus liberal view in post-World War II American political thought collapsed among public intellectuals during the tumult of the 1960s and remains so to this day. His book examines the resultant diversity among contemporary public intellectuals, focusing on three predominant themes: concern for community, worry about the environment, and interest in civil society. In marked contrast to these disputatious commentators, Fowler finds the realm of popular opinion to be characterized by much greater consensus. Indeed, there seems to be a trend toward an even more general embrace of the liberal values that characterize our attitudes toward the individual, individual liberty, political equality, economic opportunity, and consent of the governed. Liberal values—above all the celebration of the individual and individual rights—have revolutionized the so-called private realms of life like family and religious communities to an extent unimagined in the 1950s. From these conclusions, Fowler demonstrates that most interpretations of American political thinking have exaggerated the extent of conflict and diversity in our nation's often raucous policy disputes. But he also cautions us not to overstate the public's widely shared liberal values and, by doing so, miss opportunities to facilitate problem solving or to recognize the ways in which our reform efforts may be constrained

    Stadi della fede e trasformazioni della vita adulta negli studi di J. Fowler

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    J.W. Fowler (1940-2015), Ministro della Chiesa unitariana metodista, docente di teologia all’Università di Emory (Atalanta, Georgia, USA), Direttore del Centro di Ricerca sulla fede e lo sviluppo morale presso quella stessa Università, fino al 2005, è un autore ancora non tradotto in Italia, per quanto abbastanza studiato a livello internazionale. Il saggio lo introduce al lettore italiano, analizzando principalmente un suo volume del 1984 (rieditato e revisionato nel 2000), Becoming Adult, Becoming Christian (New York, Jossey Bass/ Wiley). Fowler ha utilizzato la ricerca psicologica sullo sviluppo umano per ridefinire le fasi evolutive della religiosità, sia negli stadi evolutivi, sia negli stadi adulti. Il suo modello dipende dalla letteratura scientifica quanto da una lunga esperienza di consulenza personale e di azione pastorale. L’A. evidenzia l’estrema attualità e l’importanza degli studi di Fowler per la ridefinizione del senso religioso/religiosità, in tempi di cambiamenti rapidi e di multi-cultura, e le implicazioni, implicite ed esplicite che tali studi presentano, sia per l’educazione religiosa iniziale, sia per l’accompagnamento formativo negli stadi della vita adulta. Stages of faith and transformation of adult life in the studies of J. Fowler J. W. Fowler (1940-2015), Minister of the Methodist Church Unitarian, professor of theology at Emory (Atalanta, Georgia, USA), Director of the Research Center on faith and moral development at the same university, until 2005, is an author still not translated in Italy, as studied enough at the international level. The essay introduces the reader Italian, mainly by analyzing a book of 1984 (re-edited and revised in 2000), Becoming Adult, Becoming Christian (New York, Jossey Bass / Wiley). Fowler has used psychological research on human development to redefine the evolutionary phases of religion, both in the developmental stages, both in the adult stages. His model depends on the scientific literature as a long experience of personal counseling and pastoral action. The A. highlights the extreme relevance and importance of the studies of Fowler to the redefinition of the religious sense / religiosity, in times of rapid change and multi-culture, and the implications, both implicit and explicit that such studies have, both for early religious education and for training and counseling in the stages of adult life

    Resistance Distances in Fullerene Graphs

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    Resistance distances are computed for all 1812 C60 fullerenes and found to correlate with the number of pentagon adjacencies and hence with relative energy. Within the set, the unique isolated-pen-tagon isomer has the lowest resistance distance (RT = 479482/209), the lowest Wiener index (W = 8340) and the highest Balaban index (J = 2025/2224). The most stable C40 fullerene isomer, one of two with the lowest achievable number of pentagon adjacencies, also has the lowest resistance distance in the set
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