106 research outputs found
The application of molecular biology techniques to analyse diversity in Theileria parva populations in Zambia
No full textThis thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University.Theileria parva is a complex protozoan parasite causing East Coast fever in Eastern and Central Africa. Vaccination using live parasites is an effective control measure and has been used in Zambia based on locally isolated and introduced T. parva stocks. Diversity among T. parva populations was investigated in parasites from two Zambian provinces with different disease epidemiologies and control histories. Isolates from the pre-vaccination era, local and exotic stocks used for vaccination, and one recent field isolate were cloned and passaged in vitro to study genomic stability over time.
The results of the data from three genome-wide probes indicate a marked homogeneity and stability among the Zambian isolates in contrast to East African isolates. Results from Southern blot profiles and the polymorphic immunodominant molecule (PIM) sequence analysis suggest a common origin for the Zambian isolates from the pre-vaccination era, except for one isolate (Zam5) from Southern Province. This isolate showed characteristics suggesting a buffalo origin. Assays for genotype characterisation were developed using five allelic markers. Multilocus characterisation revealed identical profiles in a recent Zambian isolate from Southern Province and two components of an exotic cocktail vaccine, indicating the escape of one of the vaccine stocks in the field.
Characterisation of T. parva field populations by RFLP-PCR assays after immunisation revealed the presence of dominant genotypes from those that had been used for vaccination. Circumstantial evidence for the involvement of one of the exotic vaccine parasites in epidemics in Southern Province is presented and a hypothesis formulated for the rapid spread of this genotype. Analysis of the characterisation data suggested the existence of two groups of T. parva parasites of different origin. The classic T. parva group, characterised by a dimorphism of the p150, p104 and p32 loci and the absence of a p67 insert and a buffalo-derived group which showed a polymorphism of p150, p104 and p32 and the presence of a p67 insert. There is evidence that recombination occurs, resulting in parasites that have characteristics of both groups. The relevance of these recombinant parasites in the epidemiology of the disease seems low. Characterisation of larger samples from areas of regular buffalo-cattle contact is necessary to clarify this. Sequence analysis of the most discriminative locus (PIM) was undertaken and gene conversion could be the main mechanism generating diversity. A more appropriate nomenclature for T. parva is proposed based on the growing evidence of molecular differences among isolates and stocks.Belgian Administration for Development
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Air flow anomaly detection
No full textAuthor Parham ParvaMasterarbeit Johannes Kepler Universität Linz 2024Arbeit nach Ablauf der Sperre auf den öffentlichen PCs in den Bibliotheken der JKU+Medizin abrufba
Air flow anomaly detection
No full textAuthor Parham ParvaMasterarbeit Johannes Kepler Universität Linz 2024Arbeit nach Ablauf der Sperre auf den öffentlichen PCs in den Bibliotheken der JKU+Medizin abrufba
Correspondence to the Atlanta Inquirer from Johnny Parham, circa 1960
No full textThis is a handwritten letter to the Atlanta Inquirer from Johnny Parham. The letter is about a proposed agreement about ceasing the non-violent protest to desegregate schools. Parham describes his apprehensive sentiments about the agreement and its integrity. He is concerned that the movement would be in vain if the agreement was signed. The agreement is a compromise invalidating African Americans' legal entitlement to equal rights. Parham mentions the student movement, and African Americans have moved passed compromises but are in a stage of demanding their equal rights. 2 pages
Dr. Robert Threatt, Interviewed by Loretta Parham, September 24, 2012
No full textVideo interviews with a complementing monograph providing reflections of former presidents of Historically Black Colleges and Universities discussing leadership, mission, challenges, successes, and issues of race and education. Interviewer: Loretta Parham, CEO & Library Director, Atlanta University Center Robert W. Woodruff Library. Interviewee: Dr. Robert Threatt, President, Morris Brown College 1973-1991
Correspondence to the Atlanta Inquirer from Johnny Parham on Prejudice, circa 1960
No full textThe handwritten document is a letter to the Atlanta Inquirer accounting for prejudicial practices and how prejudice strikes minority settings and communities. Parham describes the nature of prejudice as being unpredictable, and that it cannot be singularly directed. The letter begins with a reference to an incident of anti-Semitism and the bombing of a synagogue by Neo-Nazis and describes the prohibition of the entry of a young Jewish man into a social invite because he was Jewish. The author connects it to the plight of negroes and establishes that there is ineffective legislation to protect others from such prejudiced acts against African Americans and other minorities. 4 pages
Dr. Johnnetta B. Cole, Interviewed by Loretta Parham, June 14, 2012
No full textVideo interviews with a complementing monograph providing reflections of former presidents of Historically Black Colleges and Universities discussing leadership, mission, challenges, successes, and issues of race and education. Interviewer: Loretta Parham, CEO & Library Director, Atlanta University Center Robert W. Woodruff Library. Interviewee: Dr. Johnnetta B. Cole, President, Spelman College 1987-1997; President, Bennett College 2002-2007
Dr. Joseph B. Johnson, Interviewed by Loretta Parham, September 17, 2012
No full textVideo interviews with a complementing monograph providing reflections of former presidents of Historically Black Colleges and Universities discussing leadership, mission, challenges, successes, and issues of race and education. Interviewer: Loretta Parham, CEO & Library Director, Atlanta University Center Robert W. Woodruff Library. Interviewee: Dr. Joseph B. Johnson, President, Grambling State University 1997-1991; President, Talladega College 1991-1998
Dr. Robert M. Franklin Jr., Interviewed by Loretta Parham, August 18, 2012
No full textVideo interviews with a complementing monograph providing reflections of former presidents of Historically Black Colleges and Universities discussing leadership, mission, challenges, successes, and issues of race and education. Interviewer: Loretta Parham, CEO & Library Director, Atlanta University Center Robert W. Woodruff Library. Interviewee: Dr. Robert M. Franklin Jr., President, Interdenominational Theological Center 1997-2002; President, Morehouse College 2007-2012
Dr. Barbara R. Hatton, Interviewed by Loretta Parham, September 24, 2012
No full textVideo interviews with a complementing monograph providing reflections of former presidents of Historically Black Colleges and Universities discussing leadership, mission, challenges, successes, and issues of race and education. Interviewer: Loretta Parham, CEO & Library Director, Atlanta University Center Robert W. Woodruff Library. Interviewee: Dr. Barbara R. Hatton, President, South Carolina State University 1992-1995; President, Knoxville College 1997-2005
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