1,720,958 research outputs found
Changes in reach-to-grasp behaviour over the course of training in rats
No full textOne complex task involving sequence of movements and movement refinement in the rat is the single-pellet reaching task, comprising orientation, transport and withdrawal in sequence. In turn, orientation comprises front wall detection, slot localization and nose poke until reach start. Video recordings of a rat in the reaching box highlighted three stages of temporal training: start of training (ST), forepaw dominance appearance (D) and fully trained (T). Regarding orientation, ST versus D and T presented a significant smaller frequency of approach to the front wall and a significant higher number of whisker cycles and nose touches during slot localization, involving a significant longer Orientation. At the ST stage, 44% of the trials were interrupted after nose poke, and poke took place at significant higher level from the shelf. The shelf was identified only when short whiskers contacted it, but the tongue and both forepaws were used without distinction to reach and grasp the pellet until a forepaw emerged as dominant at D stage. Regarding the temporal features of transport and withdrawal, comparing the D versus T stage revealed a significant longer duration. Finally, successes were significantly higher in T respect to D, meaning that after dominance emergence, more training was still necessary to improve reaching/grasping performance. This study provides evidence that, during training, the rats develop a strategy to obtain the pellets and then refine their movement pattern
The effects of olfactory bulb removal on single-pellet skilled reaching task in rats
No full textWe focused on how the rat uses olfactory cues in a single-pellet reaching task, which is composed of three successive learned responses, Orient, Transport, and Withdrawal. Orient comprised: front wall detection, slot localisation, and nose poke until reach start. High-speed video-recording enabled us to describe the temporal features of this sequence in controls vs. 3–5 and 12–14 days after bilateral bulbectomy in trials with (P trial) vs. without (no-P trial) pellet. In controls, the full sequence was complete in P trials, while it was interrupted after Orient in no P-trials. After bulbectomy, the full sequence was seen in both P and no-P trials at days 3–5 and 12–14 and there was an increase in Orient duration due to the increased time in slot/shelf localisation. Unlike in controls, in anosmic rats, the first nose contact with the front wall took place below the slot/shelf level, and the number of nose touches together with the number of whisker cycles was significantly higher at 3–5 but not at 12–14 days. The relationship between nose touches and whisker cycles was linear in all experimental conditions. Bulbectomy resulted in no changes in the Transport duration or the time the paw spent out of the slot. These findings suggest that olfaction allows the animal to orient itself in pellet localisation, and offers insight into the contribution of olfaction during different stages of natural behaviour in skilled reaching task
Long and short whiskers differently guide snout/pellet interaction in rat oral grasping
No full textWe studied the role of rat whisker/snout tactile sense during oral grasping, comparing control data with those obtained, respectively, 1-3 and 5-7 days after bilateral long or short whisker trimming and 3-5 and 8-10 days after bilateral infraorbital nerve (ION) severing. Two behavioural phases were identified: whisker-snout contact by nose-N or lip-L and snout-tongue contact. The second phase involved either: snout passing over stationary pellet (Still pellet); pellet rolling as the snout passed over it (Rolling pellet); pellet being pushed forward by the snout (Pushed pellet); or pellet being hit and pushed away (Hit/Lost pellet). In controls, success was 100%, with N-contact prevailing over L-contact in the first phase and Still pellet in the second. In long whisker-trimmed versus controls, success was still 100%, but L-contact increased in frequency, Pushed pellet prevailed and the second phase duration increased. In short whisker-trimmed versus controls, success remained 100%, with increased L-contact frequency; the first phase duration did not change, but the second phase increased since in pushed trials, the pellet rolled around the snout. In ION-severed versus controls, both phases changed drastically: L-contact frequency increased, Pushed pellet prevailed and contact was persistently maintained; Hit/Lost pellet emerged, Still and Rolling pellets disappeared and the oral-grasping sequence was not triggered. These results suggest that long and short whiskers, respectively, optimize the first and second phases of snout-pellet interaction and that whisker/snout sense is necessary to trigger oral grasping. Kinematic trajectory analysis supports the conclusion that movement from whisker to snout contact is an orientation response
Adaptive changes in the motor cortex during and after longterm forelimb immobilization in adult rats
No full textExperimental and clinical studies have attempted to evaluate the changes in cortical activity seen after immobilization-induced longterm sensorimotor restriction, although results remain controversial. We used intracortical microstimulation (ICMS), which provides topographic movement representations of the motor areas in both hemispheres with optimal spatial characterization, combined with behavioural testing to unravel the effects of limb immobilization on movement representations in the rat primary motor cortex (M1). Unilateral forelimb immobilization in rats was achieved by casting the entire limb and leaving the cast in place for 15 or 30 days. Changes in M1 were bilateral and specific for the forelimb area, but were stronger in the contralateral-to-cast hemisphere. The threshold current required to evoke forelimb movement increased progressively over the period in cast, whereas the forelimb area size decreased and the non-excitable area size increased. Casting resulted in a redistribution of proximal/distal movement representations: proximal forelimb representation increased, whereas distal representation decreased in size. ICMS after cast removal showed a reversal of changes, which remained partial at 15 days. Local application of the GABAA-antagonist bicuculline revealed the impairment of cortical synaptic connectivity in the forelimb area during the period of cast and for up to 15 days after cast removal. Six days of rehabilitation using a rotarod performance protocol after cast removal did not advance map size normalization in the contralateral-to-cast M1 and enabled the cortical output towards the distal forelimb only in sites that had maintained their excitability. These results are relevant to our understanding of adult M1 plasticity during and after sensorimotor deprivation, and to new approaches to conditions that require longterm limb immobilization
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Whisker and Nose Tactile Sense Guide Rat Behavior in a Skilled Reaching Task.
Full text linkSkilled reaching is a complex movement in which a forelimb is extended to grasp food for eating. Video-recordings analysis of control rats enables us to distinguish several components of skilled reaching: Orient, approaching the front wall of the reaching box and poking the nose into the slot to locate the food pellet; Transport, advancing the forelimb through the slot to reach-grasp the pellet; and Withdrawal of the grasped food to eat. Although food location and skilled reaching is guided by olfaction, the importance of whisker/nose tactile sense in rats suggests that this too could play a role in reaching behavior. To test this hypothesis, we studied skilled reaching in rats trained in a single-pellet reaching task before and after bilateral whisker trimming and bilateral infraorbital nerve (ION) severing. During the task, bilaterally trimmed rats showed impaired Orient with respect to controls. Specifically, they detected the presence of the wall by hitting it with their nose (rather than their whiskers), and then located the slot through repetitive nose touches. The number of nose touches preceding poking was significantly higher in comparison to controls. On the other hand, macrovibrissae trimming resulted in no change in reaching/grasping or withdrawal components of skilled reaching. Bilaterally ION-severed rats, displayed a marked change in the structure of their skilled reaching. With respect to controls, in ION-severed rats: (a) approaches to the front wall were significantly reduced at 3-5 and 6-8 days; (b) nose pokes were significantly reduced at 3-5 days, and the slot was only located after many repetitive nose touches; (c) the reaching-grasping-retracting movement never appeared at 3-5 days; (d) explorative paw movements, equal to zero in controls, reached significance at 9-11 days; and (e) the restored reaching-grasping-retracting sequence was globally slower than in controls, but the success rate was the same. These findings strongly indicate that whisker trimming affected Orient, but not the reaching-grasping movement, while ION severing impaired both Orient (persistently) and reaching-grasping-retracting (transiently, for 1-2 weeks) components of skilled reaching in rats
Differential motor neuron activity in rats during successful and failed grasping
No full textA substantial body of literature has focused on neural signals evoked by errors emerging during the execution of goal-directed actions. It is still unclear how motor cortex activity during movement execution relates to feedback error processing. To investigate this, we recorded primary motor cortex (M1) single-unit activity in rats during a grasping task. About half of the recorded neurons showed modulation of their firing activity that did not depend on success or failure, which we termed outcome-independent neurons. Other neurons showed a difference in their discharge profile when comparing successful and unsuccessful trials, which we called outcome-dependent neurons. Among both outcome-dependent and -independent neurons, we further distinguished neurons presenting their maximum firing rate in specific epochs as defined by the task. We compared the cortical distribution of outcome-independent and outcome-dependent neurons to cortical maps of complex forelimb movements evoked by intracortical microstimulation in additional animals. The majority of outcome-independent neurons was localized within the limb extension and paw open-closure movement representations. Outcome-dependent neurons were not clearly associated to particular motor representations. Cortical arrangement of neurons, both outcome-independent and outcome-dependent, and their correlation with distinct movement representations, can serve as indicator for anticipating potential outcomes before the conclusion of an action
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