1,721,031 research outputs found
Glycocomposition of the apocrine interdigital gland secretions in the fallow deer (Dama dama)
No full textThe secretions of the tubular interdigital glands were investigated by conventional (PAS, Alcian-Blue at different pH, Low and High Iron Diamine) and lectin (Con-A, UEA-I, LTA, WGA, GSA-II, GSA-IB4, SBA, PNA, ECA, DBA, MAL-II and SNA) histochemical methods in adult males and females of different age of fallow deer during the breeding season. Sialidase digestion and deacetylation pre-treatment were also employed in conjunction with lectin histochemistry. The glandular epithelium consisted of a single layer of low columnar cells with typical apical protrusions. No substantial differences of the above histochemical staining in relation to sex and age were observed. Conventional histochemical staining revealed that the interdigital glands secreted neutral glycoproteins whereas acidic glycocomponents did not seem to be present. Lectin histochemical technique allowed us to disclose a great heterogeneity of glycoproteins with N- and O-linked oligosaccharides containing a-D-Man/a-D-Glc, GlcNAc, a-Fuc, terminal b-D-Gal-(1-3)-D-GalNAc, -D-Gal-(1-4)-D-GlcNAc, a-Gal and b-GalNAc residues. b-GalNAc and disaccharide b-D-Gal-(1-3)-D-GalNAc were also found as subterminal to sialyl moieties. The lack of sexual and age-related differences in the glucidic content of the glandular secretions seems to indicate that the glycoderivatives may play only an accessory role in the production of odoriferous signals in fallow deer
Characterization of glycoconjugates in the secretory epithelium of equine ampulla ductus deferentis
No full textThe present work was undertaken to
determine the glycoconjugates secreted by the
epithelium of the equine ampulla ductus deferentis,
using conventional (PAS, AB pH 2.5, AB pH 1.0) and
lectin histochemical procedures in conjunction with
enzymatic digestion and chemical treatment. The
presence of abundant apical cytoplasmic blebs suggests
that the equine ampulla secretes its products mainly in
an apocrine manner. Glandular cells secrete neutral and
acidic sialylated glycoconjugates as revealed by
conventional histochemical procedures. Lectin
histochemistry helped us to discover the following
histological positive sites: the mucosal cells, the
glandular epithelial cells, the apical cytoplasmic blebs
and the basal cells. The ampullary secretions contained
both glycoproteic material (revealed by Con-A-, LCA-,
GSA-II-, WGA-, RCA-I- positivity) and sialomucins
(evidenced by the reactivity of GSA-II, SBA, PNA and
RCA-I after sialidase digestion) having different
functional roles. The mucosal cells reacted with Con-A,
LCA, and also with sialidase/GSA-II-, SBA-, PNA-, and
RCA-I sequences, contributing to the chemical
heterogeneity of ampullary secretions. DBA lectin was a
specific marker for basal cells.
The results obtained were compared with our
previous findings regarding the differences in the lectin
binding pattern of the plasma-membrane of equine
sperm collected from epididymal cauda and the ampulla
ductis deferentis. Our results support other studies that
indicate that ampullary secretions are involved in
altering the plasma-membrane glycoconjugates of
spermatozoa, contributing to their maturation
A NOVEL APPROACH FOR IMPLEMENTING OF A LOG-SIGMOID FUNCTION ON A FPGA DEVICE USING THE SFLOAT24 MATH LIBRARY – AN MODELLING –
No full textAn Application in Bank Credit Risk Management System employing a BP Neural Network based on sfloat24 custom math library using a low cost FPGA device
No full textGlycoconjugates in small antral ovarian follicles of the river buffalo (Bubalus bubalis L.)
No full textTypes and distribution patterns of glycoconjugates in antral ovarian follicles were investigated in the buffalo, using periodic-acid Schiff (PAS), high iron diamine (HID), low ion diamine (LID) and lectin histochemical staining methods. HID and LID staining procedures were preceded in some cases by digestion with testicular hyaluronidase, Streptomyces hyaluronidase, chondroitinase ABC and heparitinase (heparinase III). Lectin staining was performed with the use of 12 horseradish peroxidase (HRP) lectin conjugates. Some lectin staining procedures were preceded by neuraminidase digestion and saponification. Large amounts of isomeric chondroitin sulphates and a minor quantity of heparan sulphate and hyaluronic acid and/or chondroitin were found in follicular fluid. Lectin staining of buffalo follicular fluid revealed glycoconjugates with different glucidic determinants such as beta-N-acetylgalactosamine, beta-galactose-(1-3)-N-acetylgalactosamine, beta-galactose(1-4)-N-acetylglucosamine, N-acetylglucosamine, alpha-fucose and alpha-glucose/alpha-mannose, and sialic acid residues. Glycosaminoglycans were absent in the zona pellucida of oocytes in small antral follicles. Acidic glycoconjugates in the zona pellucida were caused by sulphated groups and sialic acid residues. Our data show few internal glucidic residues, such as N-acetylglucosamine in the buffalo zona pellucida but many subterminal beta-N-acetylgalactosamine, alpha- and beta-galactose determinants masked by sialic acids. These findings demonstrate that buffalo follicular fluid has a very heterogeneous composition that is similar to that found in small and large bovine follicles. No differences in composition of the follicular fluid were observed in the follicles examined
Histochemistry of carbohydrates expressed by the digestive apparatus of the Umbrina cirrosa (L.) fries.
No full textLectin-binding affinity to the zona pellucida of buffalo (Bubalus bubalis) antral oocytes
No full textCorrigendum to “Presence and function of kisspeptin/KISS1R system in swine ovarian follicles” (Theriogenology (2018) 115 (1–8), (S0093691X1830147X), (10.1016/j.theriogenology.2018.04.006))
No full textThe authors regret the following changes to the author group G. Basinia, F. Grassellia, S. Bussolatia, R. Ciccimarraa, M. Maranesib, A. Bufalarib, C. Dall'Agliob, F. Parilloc,#, M. Zeranib,c,*. a Dipartimento di Scienze Mediche Veterinarie, Università di Parma, 43126 Parma, Italy. b Dipartimento di Medicina Veterinaria, Università di Perugia, 06126 Perugia Italy. c Scuola di Bioscienze e Medicina Veterinaria, Università di Camerino, 62024 Matelica Italy. # Deceased. * Corresponding author: tel.: +39 0755857642; fax +39 0755857654. E-mail address: [email protected] (M. Zerani). And to the acknowledgements and figures
Lectin-staining pattern in extratesticular rete testis and ductuli efferentes of prepubertal and adult horses
No full textThis study was undertaken to determine the lectin affinity of the extratesticular rete testis and ductuli efferentes epithelial cells in adult and prepubertal horses, using ten different lectin horseradish peroxidase conjugates: Con-A, LCA, WGA, GSA-II, SEA, PNA, RCA-I, DBA, UEA-I, and LTA. In some cases, treatments with sialidase and KOH preceded the lectin staining. In sexually mature and immature horses the results showed the presence of different kinds of sialoglycoconjugates with the terminal sialic acid linked to D-Ga1NAc and beta-D-Gal residues in the rete testis. In the apical surface and cytoplasm of epithelial cells lining the ductuli efferentes of the adult horse, glycoconjugates with alpha-D-Man and/or alpha-D-Glc, GlcNAc, D-GalNAc and beta-D-Gal residues were evidenced, whereas in the prepubertal horse only the apical surface of the ductuli efferentes epithelial cells resulted reactive toward some lectins. The differences observed in the presence of glycoconjugates between adult and prepubertal horse ductuli efferentes, suggest a hormonal control of the function of these tracts of the post-testicular ducts
A SVM ALGORITHM FOR TORQUE CONTROL OF INVERTER FED INDUCTION MOTOR DRIVE
No full textIn the paper a Space Vector Modulation (SVM) algorithm suitable for torque and flux control of induction motor fed by a VSI is described. It allows the generation of the inverter switching patterns as a function of the reference values both of the electromagnetic torque and of the controlled flux. Experimental comparisons with traditional direct torque control and field oriented control techniques are performed
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