441 research outputs found
Letter from unknown author to Michel-Dmitri Calvocoressi, undated
No full textAn undated letter from an unknown author to French critic and musicologist Michel-Dmitri Calvocoressi
Letter from unknown author to Michel-Dmitri Calvocoressi, undated
No full textAn undated letter from an unknown author to French critic and musicologist Michel-Dmitri Calvocoressi
Letter from unknown author to Michel-Dmitri Calvocoressi, undated
No full textAn undated letter from an unknown author to French critic and musicologist Michel-Dmitri Calvocoressi
Single cell analyses of ES cells reveal alternative pluripotent cell states and molecular mechanisms that control self-renewal
No full textAnalyses of gene expression in single mouse embryonic stem cells (mESCs) cultured in serum and LIF revealed the presence of two distinct cell subpopulations with individual gene expression signatures. Comparisons with published data revealed that cells in the first subpopulation are phenotypically similar to cells isolated from the inner cell mass (ICM). In contrast, cells in the second subpopulation appear to be more mature. Pluripotency Gene Regulatory Network (PGRN) reconstruction based on single-cell data and published data suggested antagonistic roles for Oct4 and Nanog in the maintenance of pluripotency states. Integrated analyses of published genomic binding (ChIP) data strongly supported this observation. Certain target genes alternatively regulated by OCT4 and NANOG, such as Sall4 and Zscan10, feed back into the top hierarchical regulator Oct4. Analyses of such incoherent feedforward loops with feedback (iFFL-FB) suggest a dynamic model for the maintenance of mESC pluripotency and self-renewal
Retelling Dmitri Karamazov’s Story in an Interactive Graphic Novel
No full textThis thesis discusses the subject and media of Dmitri Karamazov an interactive graphic novel with Augmented Reality component. Dmitri Karamazov is adapted from Dostoevsky’s novel the Brothers Karamazov. The author uses a fannish, feminine reading strategy to interpret Dostoevsky's character Mitya, transforms the original narrative and retells the story with the assistance of AR technology. The use of AR in Dmitri Karamazov highlights the fanfiction nature of this interactive graphic novel. It shows how a reader can actively participate in literary interpretation, criticism, writing, rewriting, adapting and creating in a new layer of reality. In terms of literature appreciation and consumption, AR encourages people to break away from their traditional passive-reader roles, and provides a virtual space for people to assume authorship of the materials they encounter.</p
Eksperimentasi Permasalahan Teknik-teknik pada Cello Concerto No.1 Bagian Pertama “Allegretto” Karya Dmitri Shostakovich
No full textABSTRAK Penguasaan teknik bagi seorang resitalis merupakan suatu keharusan, sesuai dengan tingkat kesulitan teknis yang ingin dicapai. Penulis mengacu pada penandaan teknis oleh pemain cello Mstislav Rostropovich untuk fingering dan bowing dalam repertoar pertama Cello Concerto No. 1 karya Dmitri Shostakovich yang kemudian ditemukan ketidakcocokan dalam preferensi pertimbangan teknis dan membuat penulis ingin mencoba bereksperimen untuk memecahkan masalah kesulitan teknis dalam repertoar tersebut. Technical Problems Experimentation in The Cello Concerto No.1, First Movement "Allegretto" by Dmitri Shostakovich ABSTRACT A recitalist's mastery of technique is a must, according to the level of desired achievements of technical difficulties. The author refers to the technical markings by cellist Mstislav Rostropovich for fingerings and bowings in the first cello concerto No.1 by Dmitri Shostakovich, which later was found discrepancies in the preferences of technical considerations, thus have made the author want to try to solve technical problems in the repertoire in a more personal way.
ClusterDraw web server: a tool to identify and visualize clusters of binding motifs for transcription factors
No full textEnergy spectrum measured by the telescope array surface detector
No full textTwo conflicting measurements of the ultra high energy cosmic ray (UHECR) flux have been reported by the Akeno Giant Air Shower Array (AGASA) and the High Resolution Fly's Eye (HiRes) experiments. HiRes observes a 5 suppression at eV, which is in agreement with the prediction of Greisen-Zatsepin-Kuz'min (GZK) theory. AGASA, in contrast, sees the flux extended well beyond eV with no visible break, suggesting that the flux is limited only by the rate at which the sources can produce the UHECR and not by interaction of energetic particles with the cosmic microwave background, thus challenging the relativistic invariance principle. In response to this discrepancy, a new experiment named the Telescope Array (TA) has been deployed, which combines the detection elements used separately by HiRes and AGASA. We describe the TA surface detector (SD) analysis using a technique new to the field, which consists of a detailed Monte-Carlo (MC) simulation of the SD response to the natural cosmic rays, validating the MC by comparing its distributions with the data, and calculation of the SD aperture from the MC. We will also describe our reconstruction procedure, based solely upon the data, and its application to both data and the MC. Finally, we will describe the energy spectrum resulting from this analysis, which is found to be in excellent agreement with the HiRes result, and as such, is the first confirmation of the GZK effect by a ground array of scintillation counters.Ph. D.Includes bibliographical referencesby Dmitri Ivano
Temporal waves of coherent gene expression during <i>Drosophila</i> embryogenesis
No full textAbstract
Motivation: Animal development depends on localized patterns of gene expression. Whole-genome methods permit the global identification of differential expression patterns. However, most gene-expression-clustering methods focus on the analysis of entire expression profiles, rather than temporal segments or time windows.
Results: In the current study, local clustering of temporal time windows was applied to developing embryos of the fruitfly, Drosophila melanogaster. Large-scale developmental events, involving temporal activation of hundreds of genes, were identified as discrete gene clusters. The time-duration analysis revealed six temporal waves of coherent gene expression during Drosophila embryogenesis. The most powerful expression waves preceded major morphogenetic movements, such as germ band elongation and dorsal closure. These waves of gene expression coincide with the inhibition of maternal transcripts during early development, the specification of ectoderm, differentiation of the nervous system, differentiation of the digestive tract, deposition of the larval cuticle and the reorganization of the cytoskeleton during global morphogenetic events. We discuss the implications of these findings with respect to the gene regulatory networks governing Drosophila development.
Availability: Data and software are available from the UC Berkeley web resource http://flydev.berkeley.edu/cgi-bin/GTEM/dmap_dm-ag/index_dmap.htm
Contact: [email protected]
Supplementary information: Supplementary data are available at Bioinformatics online.</jats:p
Time warping of evolutionary distant temporal gene expression data based on noise suppression
No full textAbstract Background Comparative analysis of genome wide temporal gene expression data has a broad potential area of application, including evolutionary biology, developmental biology, and medicine. However, at large evolutionary distances, the construction of global alignments and the consequent comparison of the time-series data are difficult. The main reason is the accumulation of variability in expression profiles of orthologous genes, in the course of evolution. Results We applied Pearson distance matrices, in combination with other noise-suppression techniques and data filtering to improve alignments. This novel framework enhanced the capacity to capture the similarities between the temporal gene expression datasets separated by large evolutionary distances. We aligned and compared the temporal gene expression data in budding (Saccharomyces cerevisiae) and fission (Schizosaccharomyces pombe) yeast, which are separated by more then ~400 myr of evolution. We found that the global alignment (time warping) properly matched the duration of cell cycle phases in these distant organisms, which was measured in prior studies. At the same time, when applied to individual ortholog pairs, this alignment procedure revealed groups of genes with distinct alignments, different from the global alignment. Conclusion Our alignment-based predictions of differences in the cell cycle phases between the two yeast species were in a good agreement with the existing data, thus supporting the computational strategy adopted in this study. We propose that the existence of the alternative alignments, specific to distinct groups of genes, suggests presence of different synchronization modes between the two organisms and possible functional decoupling of particular physiological gene networks in the course of evolution.</p
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