1,720,968 research outputs found
Estudio morfológico y molecular de especies de monilinia bajo condiciones controladas en la provincia de Mendoza
Full text linkPosterEEA MendozaFil: Rodríguez Romera, Mariela Carmen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Paolinelli, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Paolinelli, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: González Erbin, Omar Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Diaz, Mariano Emanuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; ArgentinaFil: Diaz, Mariano Emanuel. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; ArgentinaFil: Pizzuolo, Pablo. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias; Argentin
The role of melanin in the grapevine trunk disease pathogen Lasiodiplodia gilanensis
Full text linkLasiodiplodia (Botryosphaeriaceae) includes fungi that are considered
among the most aggressive to grapevine, capable of causing cankers and necrotic
lesions which eventually lead to death of host plants. A common characteristic of this
genus is the presence of melanin in conidia and mycelium. Melanin is produced by
the oxidation of phenolic and/or indolic compounds. For some fungi, this pigment is
an essential factor for pathogenicity. This study characterized the types and the roles
of melanin produced by Lasiodiplodia gilanensis. Using specific melanin inhibitors, L.
gilanensis was shown to synthesize DOPA-melanin, DHN-melanin, and pyomelanin.
DOPA-melanin was shown to be involved in production of aerial mycelium and protection
against enzymatic lysis and oxidative stress; DHN-melanin to be involved in
ramification of mycelium when exposed to nutrient deficiency; and pyomelanin to be
related with hyphae development. The fungus used tyrosine as a precursor of DOPAmelanin
and as carbon and nitrogen sources, and produced melanin inside the piths
of infected plants. Genes involved in melanin synthesis were conserved among the
Botryosphaeriaceae, highlighting the importance of melanin in this family.EEA MendozaFil: Rangel Montoya, Edelweiss Airam. Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE). Departamento de Microbiología; MéxicoFil: Paolinelli, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Paolinelli, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina; ArgentinaFil: Rolshausen, Philippe. University of California Riverside. Department of Botany and Plant Sciences; Estados UnidosFil: Hernandez Martinez, Rufina. Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE). Departamento de Microbiología; Méxic
The Role of Diversity in Mediating Microbiota Structural and Functional Differences in Two Sympatric Species of Abalone Under Stressed Withering Syndrome Conditions
No full textWithering syndrome (WS) is a gastro-intestinal (GI) infectious disease likely affecting all abalone species worldwide. Structural and functional changes in abalone GI microbiotas under WS-stressed conditions remain poorly investigated. It is unclear if interspecific microbiota differences, such as the presence of certain microbes, their abundance, and functional capabilities, may be involved in the occurrence of this disease. Bacterial microbiotas of healthy Haliotis fulgens and Haliotis corrugata are mainly composed by Tenericutes, Proteobacteria, Fusobacteria, and Spirochaetes. We previously reported species-specific structural and functional profiles of those communities and suggested that they are of consequence to the different susceptibility of each species to WS. Here, we address this question by comparing the structure and function of healthy and dysbiotic microbiota through 454 pyrosequencing and PICRUSt 2, respectively. Our findings suggest that the extent to which WS-stressed conditions may explain structural and functional differences in GI microbiota is contingent on the microbiota diversity itself. Indeed, microbiota differences between stressed and healthy abalone were marginal in the more complex bacterial communities of H. corrugata, in which no significant structural or functional changes were detected. Conversely, significant structural changes were observed in the less complex bacterial microbiota of H. fulgens. Moreover, structural alterations led to a significant downregulation of some metabolic activities conducted by GI bacteria. Accordingly, results suggest that gastro-intestinal bacterial diversity appears to be related with both the health of abalone and the etiology of WS.EEA MendozaFil: Cicala, Francesco. CICESE. Department of Biological Oceanography; MéxicoFil: Cicala, Francesco. CICESE. Department of Biomedical Innovation; MéxicoFil: Cisterna-Céliz, José Alejandro. CICESE. Department of Biological Oceanography; MéxicoFil: Paolinelli, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Paolinelli, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moore, James D. University of California at Davis. Bodega Marine Laboratory; Estados UnidosFil: Sevigny, Joseph. University of New Hampshire. Hubbard Center for Genome Studies; Estados UnidosFil: Rocha-Olivares, Axayácatl. CICESE. Department of Biological Oceanography; Méxic
Lasiodiplodia gilanensis used as model for understanding the pathogenicity of Botryosphaeriaceae
No full textLasiodiplodia spp. cause degenerative diseases, dieback and plant death in a wide range of woody plants. To understand the fungal behavior of the genus we are using Lasiodiplodia gilanensis as a model. A transcriptional study revealed that the fungus is capable of using the phenylpropanoid precursors and salicylic acid to avoid the host defense response of the plant. On another hand, several genes encoding enzymes involved in different melanin synthesis pathways; 3,4-dihydroxyphenylalanine (DOPA)-melanin, 1,8-dihydroxynaphthalene (DHN)-melanin and pyomelanin; were identified and their production evauated, concluding that the fungus use diferent types of melanin to overcome environmental stress. An in silico analysis shows the presence of those genes in all the available genomes of Botryosphaeriaceae in the GeneBank, evidencing the importance of the melanin in this family. L. gilanensis produces siderophores of catechol and hydroxamate-type as well as naturally esterified fatty acids that might have a role in plant growth regulation. A plethora of hydrolytic enzymes is also produced, including xylanases, ligninases, cellulases, pectinases, cutinases, and hemicelluloses. Another secondary metabolite produced is oxalic acid, reported as a pathogenicity factor in other fungi, which role is under evaluation. Microscopical observations showed that the fungus uses the starch deposited in the ray cells as carbon source, induces the production of suberin and phenolic compounds and colonizes the vascular cambium, ray parenchyma, and the vascular bundles. These studies extend our understanding of the pathogenicity of a widely distributed pathogenic fungus with our final goal to control the diseases it causes.Fil: Rangel Montoya Edelweiss. Consejo Nacional de Ciencia y Tecnología de México. Centro de Investigación Científica y de Educación Superior de Ensenada Baja California; MéxicoFil: Paolinelli, Marcos. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hernández Martínez Rufina. Consejo Nacional de Ciencia y Tecnología de México. Centro de Investigación Científica y de Educación Superior de Ensenada Baja California; México11th International Workshop on grapevine trunk diseasesPentictonCanadáOregon Wine Research Institut
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Characterization of Lasiodiplodia species associated with grapevines in Mexico
Full text linkBotryosphaeria dieback is one of the most prevalent grapevine trunk diseases (GTDs), and is caused by fungi in the Botryosphaeriaceae. Fungi invade grapevine vascular systems mainly through pruning wounds, and cause cankers and necrotic
lesions, which lead to grapevine decline and death. Lasiodiplodia theobromae has been reported as a highly virulent pathogen of grapevine, and was previously reported in Mexican vineyards. The taxonomy of Lasiodiplodia was recently revised, adding new species, and some were reduced to synonymy. This study aimed to characterize Lasiodiplodia producing grapevine dieback symptoms in Sonora and Baja California, Mexico.
Using the phylogenetic markers tef1-α and ITS regions, Lasiodiplodia brasiliensis, L. crassispora, L. exigua, and L. gilanensis were identified. Lasidiplodia exigua was the most prevalent species. Lasiodiplodia brasiliensis and L. gilanensis were very virulent to
‘Cabernet Sauvignon’ plants, while L. exigua and L. gilanensis were less virulent, and L. crassispora did not produce lesions at 2 months post-inoculation. The optimum temperature of the Lasiodiplodia spp. was 28°C, but all four species grew up to 37°C, and
the isolates of L. exigua grew slowly at 40°C. This is the first report of the four of Lasiodiplodia species in vineyards of Mexico.EEA MendozaFil: Rangel Montoya, Edelweiss A. Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE). Departamento de Microbiología; MéxicoFil: Paolinelli, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Paolinelli, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Rolshausen, Philippe E. University of California, Riverside. Department of Botany and Plant Sciences; Estados UnidosFil: Valenzuela Solano, Cesar. Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP). Campo Experimental Costa de Ensenada; MéxicoFil.: Hernandez Martinez, Rufina. Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE). Departamento de Microbiología; Méxic
Characterization of grapevine wood microbiome through a metatranscriptomic approach
No full textGrapevine trunk diseases threaten wine and table grape production worldwide, primarily by reducing yields and, in its
advanced stages, causing plant death. Among those diseases, the complex etiology disease known as hoja de malvón (HDM)
significantly concerns Argentinian and Uruguayan viticulture. At least four different fungi are associated with this disease,
but their role and interactions with other wood microorganisms are understudied. In this sense, analyzing grapevine wood
microbiome composition could help understand microbial interactions occurring in HDM onset. Hence, a metatranscriptomic
study was performed for the microbiome characterization of mature field-grown Vitis vinifera cv. Malbec, leaf-symptomatic
or leaf-asymptomatic. The microbiome was mainly represented by Dothideomycetes and Actinobacteria. In the plant with
more marked symptoms, higher levels of the Basidiomycota Arambarria destruens and Phellinus laevigatus were detected.
Despite this particular difference, discriminating symptomatic from asymptomatic plants based on the presence or abundance
of HDM pathogens was not possible. Alpha diversity and rank-abundance curve analyses indicated that plants with
foliar symptoms have lower microbial evenness than asymptomatic plants. The co-occurrence network modeled microbial
interkingdom interactions. Molecular data generated in this study will help develop future targeted molecular quantification
for specific taxa.EEA MendozaFil: Paolinelli, Marcos. Instituto Nacional de Investigación Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Escoriaza, Maria Georgina. Instituto Nacional de Investigación Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Cesari, Cecilia Alicia. Instituto Nacional de Investigación Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Garcia Lampasona, Sandra Claudia. Instituto Nacional de Investigación Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Garcia Lampasona, Sandra Claudia. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias, Instituto de Biología Agrícola de Mendoza (IBAM), Mendoza, ArgentinaFil: Garcia Lampasona, Sandra Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Mendoza; ArgentinaFil: Hernandez Martinez, Rufina. Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE); Méxic
- …
