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Identification of three novel myeloid cathelicidin cDNAs and their predicted peptides in buffalo (<i>Bubalus bubalis</i>)
No full text273-277Antimicrobial peptides (AMPs) are broad spectrum
antibiotics, which mostly act without specific receptors. Identification of
AMPs is important in the current scenario of emerging multi-drug resistant
bacteria. In the present study, in an attempt to identify new AMPs, myeloid
cathelicidin cDNAs were synthesized from buffalo (Bubalus bubalis)
bone marrow and were
amplified using specific primers. Sequence analysis of cloned cDNAs revealed three
novel myeloid cathelicidins. They were named based on
the number of active amino acids in the C-terminal region of their predicted
peptide sequences as BuMAP-28 (having an additional Gly at position 22nd),
BuMAP-29 (having an additional IIe at position 27) and BuMAP-34, compared to
BMAP-27, BMAP-28 and BMAP-34 of cattle. The BuMAPs showed 93%, 95% and 87% homology respectively with that of its cattle
counterpart. Predicted number of amino acids of the cDNAs was 159, 155 and 157
residues, with cationic C-terminal sequences of 28, 29 and 34, respectively, which correspond to putative antimicrobial domains. Several amino acid substitutions were observed in all the three
cathelicidins. The conformation of the peptides was predicted to be alpha
helical, having total net positive charge and hydrophobicity, similar to that
of BMAPs in cattle. Comparative analysis of the predicted peptides suggested
potential antimicrobial activity and the sequence variations detected might
enable the peptides to act as effective broad spectrum antimicrobial agents
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Promoter sequence analysis of ATP citrate lyase gene in buffalo and Vechur cattle
No full textA study has been carried out to analyze the promoter region of ATP citrate lyase (ACLY) gene in Murrah buffalo (Bubalusbubalis) and native Vechur cattle. PCR amplification of the promoter region generated a fragment of 768 bp in both Buffalo and Vechur cattle.Sequence analysis revealed a few elements/consensus sequences, which have potential role in regulation of transcription. TATA box is found to be absent in the promoter region whereas, TATA like sequence, CAAT box, Cyclic AMP responsive element (CRE), Fat Specific Element (FSE), Enhancer core, Nuclear factor-k Binding site (NF-kB), E- box and inverted CAAT box are present. Comparison of these sequences between ruminants and non-ruminants revealed the presence of important sequence motifs such as, CRE, inverted CAAT boxes and a CAAT box situated far away from the transcription initiation site, together with the absence of a TATA box. All these findings correlate with down regulation of the gene resulting in a low level of expression in ruminants. Phylogenetic analysis of the nucleotide sequences revealed clustering of buffalo, Vechur cattle and Cattle on one clade and Rat and Human delineated from other species and clustered out on a separate clade
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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