5 research outputs found
Adenocarcinoma of humerus shaft treated with en bloc excision and long locking compression plate with fibular strut and iliac bone grafting: a case report
No full textAdenocarcinoma of the humerus shaft is a rare malignant tumor, often secondary to metastasis from a primary site such as the lung, breast, or gastrointestinal tract. Management of these cases is challenging due to the complex anatomy, the need to preserve limb function, and the high risk of recurrence. This case report highlights a unique approach involving en bloc excision, long locking compression plate (LCP) fixation, fibular strut grafting, and iliac bone grafting. A 55-year-old female presented with persistent pain and swelling in the midshaft of right humerus, accompanied by reduced range of motion. Imaging revealed a lytic lesion, and biopsy confirmed metastatic adenocarcinoma. After a multidisciplinary evaluation, an en bloc excision of the tumor was performed. Reconstruction involved internal fixation with a long LCP, augmented by a vascularized fibular strut graft and autologous iliac bone grafting. Postoperative care included adjuvant chemotherapy and physiotherapy. Postoperative outcomes were favorable, with evidence of graft integration and functional recovery at six months, alongside significant pain reduction and absence of local recurrence at one-year follow-up. The combination of biological and mechanical reconstruction techniques provided durable outcomes and restored limb function. This report underscores the importance of a multidisciplinary approach and highlights the efficacy of autograft-based biological reconstruction in managing extensive diaphyseal defects. The chosen method demonstrates a viable alternative to endoprosthetic reconstruction, particularly in patients with long-term survival potential
Investigation of Different Window and Wall Materials for Solar Passive Building Design
No full textAbstractThe energy consumption associated with the cooling of the buildings is huge. In India buildings consume about 33% of country's power production for cooling and day lighting. The building enclosures such as walls, roofs and glasses play very vital role in reducing cooling loads in the buildings. The proper combination of window glass materials and wall materials can cut down the cooling costs extensively. In the present work, five different glass materials such as clear, bronze, grey, green and blue-green glass materials were selected and four different building materials such as burnt brick, cinder concrete, dense concrete and fly ash brick either side plastered with cement plaster were selected. Total twenty building models with various combinations of window glass and wall materials were designed in licensed Design builder 4.3.0.039 version and thermal analysis was carried out in Energy plus 8.1 software package. Thermal performance of various building models in four different climatic zones such as hot and dry, temperate, warm and humid and composite were investigated. From the results of the study, it is observed that fly ash brick wall building model with grey window glass is found to be energy efficient in all Indian climatic zones from the reduced cooling load point of view among all studied combinations in East, West, North and South orientations. From the results it is observed that the fly ash brick buildings with grey glass window is observed to be the most energy efficient combination for reducing cooling loads as they gain the least heat gain in south orientation (21.51 kWh) for Ahmedabad region. The results of the study help in designing energy efficient passive buildings
Analysis of Differentially Expressed Rice Genes Reveals the ATP-Binding Cassette Transporters as Candidate Genes Against the Sheath Blight Pathogen, Rhizoctonia solani
No full textSheath blight is a serious rice disease worldwide, and genes involved in resistance remain unclear. In the present study, a virulent field isolate of Rhizoctonia solani was used to inoculate detached leaves of a sheath blight-resistant rice cultivar, Jasmine 85; a suppression subtractive cDNA library was constructed using RNA isolated 16 h postinoculation (hpi); and differentially expressed genes were identified from the cDNA library. A total of 159 uniquely expressed sequence tags were identified, including 105 from rice with enrichment in categories related to cellular response, molecular signaling, and host defense. Coupled with gene expression studies by DNA microarray, 27 highly induced genes involved in signal transduction and defense responses were identified within 16 hpi. Three members of the ATP-binding cassette (ABC) transporter gene family (OsABC1, OsABC9, and OsABC12) encoding pleiotropic drug resistance-like ABC transporters were mapped to different sheath blight resistance quantitative trait loci (QTLs), and their differential expressions were validated. Three high-resolution melting (HRM) markers were developed from these ABC gene family members to distinguish alleles between sheath blight-susceptible cultivar Lemont and resistant cultivar Jasmine 85. Association of sheath blight resistance to these HRM markers was examined in 77 recombinant inbred lines derived from the cross between Jasmine 85 and Lemont. The OsABC9 gene located in a major sheath blight resistance QTL qShB9-2 showed a major contribution to sheath blight resistance. These results are useful for marker-assisted selection and functional validation of the ABC genes in sheath blight disease resistance. [Figure: see text] The author(s) have dedicated the work to the public domain under the Creative Commons CC0 “No Rights Reserved” license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law, 2022
The Medicago truncatula Yellow Stripe1-Like3 gene is involved in vascular delivery of transition metals to root nodules
No full text13 Pags.- 5 Figs. © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology.Symbiotic nitrogen fixation carried out in legume root nodules requires transition metals. These nutrients are delivered by the host plant to the endosymbiotic nitrogen-fixing bacteria living within the nodule cells, a process in which vascular transport is essential. As members of the Yellow Stripe-Like (YSL) family of metal transporters are involved in root to shoot transport, they should also be required for root to nodule metal delivery. The genome of the model legume Medicago truncatula encodes eight YSL proteins, four of them with a high degree of similarity to Arabidopsis thaliana YSLs involved in long-distance metal trafficking. Among them, MtYSL3 is a plasma membrane protein expressed by vascular cells in roots and nodules and by cortical nodule cells. Reducing the expression level of this gene had no major effect on plant physiology when assimilable nitrogen was provided in the nutrient solution. However, nodule functioning was severely impaired, with a significant reduction of nitrogen fixation capabilities. Further, iron and zinc accumulation and distribution changed. Iron was retained in the apical region of the nodule, while zinc became strongly accumulated in the nodule veins in the ysl3 mutant. These data suggest a role for MtYSL3 in vascular delivery of iron and zinc to symbiotic nitrogen fixation.This research was funded by a European Research Council Starting
Grant (ERC-2013-StG-335284) and the Spanish State Research
Agency (AEI) grant (AGL2015-65866-P) to MGG, and a AEI grant
(AGL2016-75226-R) to JA and AA-F co-financed by the European
Regional Development Fund (FEDER). RC-R and FJJ-P were supported by a Formación del Personal Investigador fellowship (BES2013-062674 and BES-2017-082913, respectively). IA was the
recipient of a Juan de la Cierva- Formación postdoctoral fellowship
from Ministerio de Ciencia, Innovación y Universidades (FJCI-2017-
33222). VE was partially funded by the Severo Ochoa Programme
for Centres of Excellence in R&D from the AEI (grant SEV-2016-
0672) received by the Centro de Biotecnología y Genómica de Plantas
(UPM-INIA). Development of the M. truncatula Tnt1 mutant population was, in part, funded by the National Science Foundation, USA
(DBI-0703285) to KSM. AM and HK and the µXRF measurements
were supported by the Ministry of Education, Youth and Sports of
the Czech Republic with co-financing from the European Union
(grant ‘KOROLID’, CZ.02.1.01/0.0/0.0/15_003/0000336) and the Czech Academy of Sciences (RVO: 60077344).Peer reviewe
Nicotianamine synthase 2 Is required for symbiotic Nitrogen fixation in Medicago truncatula nodules
No full text14 Pags.- 6 Figs.- 1 Tabl. Copyright © 2020 The Authors. This is an open-access
article distributed under the terms of the Creative Commons Attribution License
(CC BY). The use, distribution or reproduction in other forums is permitted,
provided the original author(s) and the copyright owner(s) are credited and that
the original publication in this journal is cited, in accordance with accepted
academic practice. No use, distribution or reproduction is permitted which does
not comply with these terms.Symbiotic nitrogen fixation carried out by the interaction between legumes and diazotrophic bacteria known as rhizobia requires relatively large levels of transition metals. These elements are cofactors of many key enzymes involved in this process. Metallic micronutrients are obtained from soil by the roots and directed to sink organs by the vasculature, in a process mediated by a number of metal transporters and small organic molecules that facilitate metal delivery in the plant fluids. Among the later, nicotianamine is one of the most important. Synthesized by nicotianamine synthases (NAS), this molecule forms metal complexes participating in intracellular metal homeostasis and long-distance metal trafficking. Here we characterized the NAS2 gene from model legume Medicago truncatula. MtNAS2 is located in the root vasculature and in all nodule tissues in the infection and fixation zones. Symbiotic nitrogen fixation requires of MtNAS2 function, as indicated by the loss of nitrogenase activity in the insertional mutant nas2-1, phenotype reverted by reintroduction of a wild-type copy of MtNAS2. This would result from the altered iron distribution in nas2-1 nodules shown with X-ray fluorescence. Moreover, iron speciation is also affected in these nodules. These data suggest a role of nicotianamine in iron delivery for symbiotic nitrogen fixation.This research was funded by a European Research Council Starting Grant (ERC-2013-StG-335284) and a Ministerio de Economía y Competitividad (MINECO) grant (AGL2015-65866-P), to MG-G, and a MINECO grant (AGL2016-75226-R) to JA and AÁ-F. VE was partially funded by the Severo Ochoa Programme for Centres of Excellence in R&D from Agencia Estatal de Investigación of Spain (grant SEV-2016-0672) to CBGP. IA is recipient of a Juan de la Cierva- Formación postodoctoral fellowship from Ministerio de Ciencia, Innovación y Universidades (FJCI-2017-33222). Development of M. truncatula Tnt1 mutant population was, in part, funded by the National Science Foundation, USA (DBI-0703285) to KM.Peer reviewe
