322,830 research outputs found
Assessing environmental activism among young adults: development and validation of the YA-EAS scale
No full textIn response to the ongoing climate crisis (Hopkins, 2020), young adults have become a significant force advocating for environmental causes, engaging in a range of actions from moderate activities like sit-ins and marches to more extreme forms such as blocking traffic and symbolic artifact destruction (Arya & Henn, 2021). Understanding the extent of environmental activism among young adults is therefore crucial to channeling this passion. Although measures like the Environmental Action Scale (Alisat & Reimer, 2015) exist to assess environmental activism, many of these instruments primarily target general populations without specific consideration for young adults. Given the unique challenges this demographic faces regarding environmental issues, there is an urgent need to develop and validate measurement scales tailored explicitly to their context. This research aims to address this gap by introducing the Young Adults Environmental Activism Scale (YA-EAS), a comprehensive tool designed to measure environmental activism specifically among young adults (age 18-26). The YA-EAS aims to differentiate between extreme and non-extreme behaviors. In Study 1, an exploratory factor analysis involving 300 participants revealed a reliable scale with two factors: "extreme activism," representing engagement in radical actions, and "non-extreme activism," indicating moderate or conventional involvement in environmental causes. Additionally, to assess the scale's structural validity, participants evaluated the relevance of YA-EAS items to environmental activism and the perceived extremism of the mentioned behaviors. In Study 2, using a new sample of young adults (N=300), a confirmatory factor analysis validated the two factors identified in Study 1. Furthermore, correlations were examined between the two YA-EAS subscales and relevant variables to evaluate the scale's convergent and discriminant validity
U1 small nuclear RNA chimeric ribozymes with substrate specificity for the Rev pre-mRNA of human immunodeficiency virus
No full textThe in vivo effectiveness of ribozymes strongly depends on the correct choice of the vector molecule. High levels of expression, stability, active conformation, and correct cellular localization are the most important features for a ribozyme vector. We have exploited the utilization of the U1 small nuclear RNA (snRNA) as a vector for specifically targeting a ribozyme into the nucleus. The Rev pre-mRNA of human immunodeficiency virus type 1 was chosen as target for testing the activity of the Ul-ribozyme. The catalytic core of the hammerhead motif, plus the recognition sequences, substituted the stem-loop III of the U1 snRNA. The resulting construct displays efficient cleavage activity in vitro. In addition, in the in vivo system of Xenopus laevis oocytes, the Ul-chimeric ribozyme accumulates in large amounts in the nucleus and produces a considerable reduction of Rev pre-mRNA levels. The Rev-specific ribozyme was also inserted in a derivative of the Ul snRNA mutated in the region of pairing with the 5' splice site, such as to match it with the suboptimal splice junction of the Rev precursor. This construct shows more efficient reduction of Rev pre-mRNA in vivo than the wild-type U1 vector
Use of adenoviral VAI small RNA as a carrier for cytoplasmic delivery of ribozymes
No full textThe in vivo effectiveness of therapeutic RNAs, like antisense molecules and ribozymes, relies on several features: RNA molecules need to be expressed at high levels in the correct cellular compartment as stable and active molecules. The exploitation of "natural" small RNA coding genes as expressing cassettes gives high chances to fulfill these requirements. We have investigated the utilization of the adenoviral VAI RNA as a cytoplasmatic carrier for expressing ribozymes against HIV-1. The conserved 5' leader sequence of HIV was chosen as a target, because it is present in all the viral transcripts and is highly conserved. Hammerhead ribozymes were substituted to different portions of the VAI RNA and the resulting chimera were tested in the in vivo system of Xenopus laevis oocytes for their level of accumulation, cellular compartmentalization, and assembly in specific ribonucleoparticles containing the La antigen. Interesting differences in the activity of the different chimera were found in both in vitro cleavage assays and S100 extracts of injected oocytes where the catalytic activity of the ribozymes in the RNP context can be analyzed
Two different snoRNAs are encoded in introns of amphibian and human L1 ribosomal protein genes
No full textWe previously reported that the third intron of the X.laevis L1 ribosomal protein gene encodes for a snoRNA called U16. Here we show that four different introns of the same gene contain another previously uncharacterized snoRNA (U18) which is associated with fibrillarin in the nucleolus and which originates by processing of the pre-mRNA. The pathway of U18 RNA release from the pre-mRNA is the same as the one described for U16: primary endonucleolytic cleavages upstream and downstream of the U18 coding region produce a pre-U18 RNA which is subsequently trimmed to the mature form. Both the gene organization and processing of U18 are conserved in the corresponding genes of X.tropicalis and H.sapiens. The L1 gene thus has a composite structure, highly conserved in evolution, in which sequences coding for a ribosomal protein are intermingled with sequences coding for two different snoRNAs. The nucleolar localization of these different components suggests some common function on ribosome biosynthesis
A novel small nucleolar RNA (U16) is encoded inside a ribosomal protein intron and originates by processing of the pre-mRNA
No full textWe report that the third intron of the L1 ribosomal protein gene of Xenopus laevis encodes a previously uncharacterized small nucleolar RNA that we called U16. This snRNA is not independently transcribed; instead it originates by processing of the pre-mRNA in which it is contained. Its sequence, localization and biosynthesis are phylogenetically conserved: in the corresponding intron of the human L1 ribosomal protein gene a highly homologous region is found which can be released from the pre-mRNA by a mechanism similar to that described for the amphibian U16 RNA. The presence of a snoRNA inside an intron of the L1 ribosomal protein gene and the phylogenetic conservation of this gene arrangement suggest an important regulatory/functional link between these two components
Inhibition of human immunodeficiency virus type 1 replication by nuclear chimeric anti-HIV ribozymes in a human T lymphoblastoid cell line
No full textHuman immunodeficiency virus (HIV) infection represents one of the most challenging systems for gene therapy. Thanks to the extended knowledge of the molecular biology of the HIV life cycle, many different strategies have been developed including transdominant modifications of HIV proteins, RNA decoys, antisense RNA, ribozymes, and intracellular antibody fragments. In this paper, we have tested in a human T lymphoblastoid cell line the antiviral activity of ribozymes specifically designed to co-localize inside the nucleus with the Rev pre-mRNA before it is spliced and transported to the cytoplasm. This result was obtained by inserting the ribozyme in the spliceosomal U1 small nuclear RNA (snRNA) and in a derivative that has perfect complementarity with the 5' splice site of the Rev pre-mRNA. These ribozymes were tested in human T cell clones and were shown to be very efficient in inhibiting viral replication. Not only were the p24 levels in the culture medium drastically reduced but so were the intracellular HIV transcripts. Control disabled ribozymes enabled us to show the specificity of the ribozyme activity. Therefore, these constructs have potential utility for gene therapy of HIV-1 infection
Diffusive author(s), cohesive author: Analysis of S/N (1994)
No full textThis study indicates the ways in which various aspects of the author(s) are brought forth in Dumb type’s performance art, the S/N production. Previous research has suggested a non-hierarchical organization of Dumb type and the absence of a “privileged author” in Dumb type’s collaborative work, S/N. However, the results that I have investigated from member’s interviews on the creative process of S/N along with my analysis of the recorded images of S/N, indicate a different aspect of the author(s). First, S/N was created through, so to speak, the collective ideas of the members of Dumb type. Further, S/N has at least nine quotations from previous performances, installations, and printed writings, besides the work-in-progress technique. Explicating one of the “author functions” as given by Michel Foucault, each text has plural subjects of the author. However, it has been revealed from members’ interviews that Teiji Furuhashi had a decision-making role in selecting the members’ ideas within the performance. Since then, S/N has had plural subjects of creation; however, Furuhashi is one of the subjects of creation along with the “privileged author.” S/N has plural authors (diffusive authors) yet at the same time, it has a “privileged author,” Teiji Furuhashi (cohesive author)
Stability and Change in Intolerance of Uncertainty and Its Association with Interpretation Bias in Social Situations: A Longitudinal Study of Italian Adolescents
No full textBackground: Intolerance of Uncertainty (IU) is a transdiagnostic factor measured using the Intolerance of Uncertainty Scale-Revised (IUS-R). This study evaluated the stability and change in adolescents' IU over a three-month period using a modified version of the scale. Methods: A two-wave study was conducted, with 290 adolescents responding to an online survey at baseline and 199 at follow-up. The original IUS-R was modified to probe the rating of the current perceived state of IU, rather than typical experience. The link between IU variability and the development of interpretation bias in ambiguous social situations at follow-up was explored. Structural Equation Modeling and Linear Mixed Model analyses were performed to assess the longitudinal measurement invariance and responsiveness of the Modified IUS-R scale. Results: The scale demonstrated good psychometric properties and full measurement invariance. Individual participants showed significant variability in baseline IU levels but not in the degree of change. A reliable change in scores was observed in 8% of adolescents. The Modified IUS-R predicted interpretation bias in social situations at follow-up. Conclusions: Significant inter-individual-level variation in IU suggests this tool may be useful for detecting changes in IU and predicting significant health outcomes. Future studies should further address the assessment of changing IU with longer timeframes
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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