1,721,100 research outputs found
Osservazioni bio-etologiche su Peliococcus cycliger (Leonardi, 1908) (Homoptera: Pseudococcidae)
Peliococcus cycliger (Leonardi) (Homoptera: Pseudococcidae) produces three generations in a year in Apulia, on Olea europaea L. The mealybug is oviparous and parthenogenetic; males are rare. The mealybug dislikes high temperatures. The first generation of P. cycliger lives on the branches, the second protects itself in the bark crevices, or under Saissetia oleae (Oliv.) and Lichtensia viburni Sign. The first instar of the third generation overwinters inside waxy cocoons.
Crematogaster scutellaris Oliv. (Hymenoptera: Formicidae) attends to the mealybug and the endoparasitoid Anagyrus How. sp. (Hymenoptera, Encyrtidae) partially controls the scale.
Damage is not reported on olive-trees
SULL'ALLESTIMENTO DI PREPARATI MICROSCOPICI DI PUPARI DI ALEIRODIDI (Homoptera)
A method of preparing whitefly pupa! cases for phase contrast microscopy observations, without hea-
ting, is described.
Phase contrast observation is preferred on account of the detailed sight of morphological carachters,
without staining.
Specimens are placed in a 10% solution of KOH, without heating, for severa! hours. Then they are
placed in tetrahydrofuran, a very good wax solvent
The recovery of a forgotten copy of "Chermoteca italica". Boll. Zool. agr. Bachic. Ser.II, 33 (3) Special Issue. Proceedings of IX International Symposium on Scale Insects Studies: 510-511 (poster).
IDENTIFICATION OF A MECHANISM FOR ENZYMATIC REGULATION IN NARE
NarE (Neisseria ADP-ribosylating enzyme), identified in the virulent strain MC58 of Nesseria meningitidis, belongs to the family of bacterial ADP-ribosyltransferases [1]. These enzymes catalyze the transfer of the ADP-ribose unit from β-nicotinamide adenine dinucleotide (NAD+) to specific amino-acids (R, C, N, T, Q) with simultaneous release of nicotinamide (nam). In vitro assays showed that NarE is an arginine-specific ADP-ribosyltransferase and also possesses NAD-glycohydrolase (NADase) activity. Recently we showed that NarE contains an iron-sulfur cluster (Fe-S) [2] in which a single iron atom is coordinated with two C (C67 and C128) and two H (H46 and H57) [3]. The presence of a structured cluster is important for transferase activity, which is lost when the cluster is destroyed, while the NADase activity is only slightly decreased. Here we reported that NarE ADP-ribosylates itself in vitro in a dose and concentration dependent manner. Incubation with NH2OH and NaOH hydrolyzed the link between ADP-ribose and NarE suggesting arginine the ADP-ribose acceptor. Four arginine residues (R7, R33, R97, R124) are present in NarE, we mutated each single R to K to identify the modified residue. The mutant R7K does not bind ADP-ribose suggesting that R7 is likely the target of auto-modification. The ADP-ribose unit carries two negative charges and alters considerably the chemical property of the R-residue, which is involved in the binding of NAD and the protein substrate. So the auto-modification influences the NarE enzymatic activities, the ADP-ribosylated NarE decreases transferase activity while NADase proportionally enhances. These data provided evidence that the auto-modification is an intramolecular mechanism adopted by NarE to regulate the priority between the two enzymatic activities.
[1] Masignani V. et al Mol. Microbiology (2003) 50(3), 1055-1067
[2] Del Vecchio M. et al. J Biol. Chem. (2009) 284(48):33040-7
[3] Koehler C. et al J Biol. Chem. (2011) in pres
Valutazione dell’efficacia di alcuni insetticidi su Dociostaurus maroccanus (Thunberg) e sul suo predatore oofago Mylabris variabilis Pallas
Prove di efficacia di funghi entomopatogeni su Dociostaurus maroccanus (Thunberg) e sul suo predatore oofago Mylabris variabilis Pallas
Going Beyond Counting First Authors in Author Co-citation Analysis
The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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