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Chloropastic ycf2 Gene Expression in Stressed Plants
No full textThe chloroplast ycf2 gene plays a vital unknown function in the higher plants: gene silencing or reduction in mRNA synthesis induce cell death (Drescher et al., 2000).
Using the SSH technique, Bernardi et al. (2008) isolated a cDNA sequence in cold tolerant plants of Olea europaea L. showing a very high expression of the ycf2 gene induced by cold treatment.
The olive gene was isolated and sequenced. It presents a 6385 nucleotide length and the sequence is highly conserved in the plants, also in the rudimentary plastid genome of the non-photosynthetic parasitic Epifagus virginiana (Wolfe et al., 1992), thus confirming an essential role for the plant survival. The hypothetical protein sequence is constituted of 2278 amino acids presenting an ATPase domain and a DUF825 domain with unknown function.
We have focused our work on the fruit development of O. europaea in order to verify if ycf2 may have a specialized function in non-photosynthetic tissues during seed maturation, as reported for tomato by Richards et al. (1994). Semi-quantitative RT-PCR using mRNA extracted at different maturation stages indicated that mRNA is present in fruits of all stages, although the higher concentration was found in leaves.
Analysis of ycf2 transcripts in poplar plants treated with ozone and in plane infected with Ceratocystis fimbriata, agent of the colored canker, confirmed a differential expression of the gene after the stressing treatments.
All the results point to a defense activation of ycf2 both in biotic and abiotic stresses
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Multiple primers pairs polymerase chain reaction for the detection of Human Papillomavirus types
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Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Le difficoltà di risoluzione dei problemi matematici
No full textLe problematiche concernenti la risoluzione dei problemi, ovvero il problem solving, non sono tuttavia ancora riconosciute ufficialmente come disturbo a sé stante, ma vengono fatte ricadere nella categoria del disturbo dell’apprendimento matematico spesso definito anche discalculia. Tale disturbo è stato riconosciuto, tra gli altri, dall’Organizzazione Mondiale della Sanità e dall’American Psychiatric Association a fianco dei disturbi che interessano, ad esempio, l’area della lettura o della scrittura. Il capitolo prende in esame i processi cognitivi e metacognitivi implicati nella soluzione dei problemi, la diagnosi del disturbo e le proposte d’intervento
Il trattamento nei campus estivi per DSA
No full textGli interventi riabilitativi fino ad oggi proposti sono molto diversi fra loro per modelli di riferimento, obiettivi e metodi. Alcuni interventi sono specializzati per agire su una funzione cognitiva specifica (ad esempio l’intervento di tipo fonologico), mentre altri hanno obiettivi che si riferiscono alla riabilitazione di molteplici funzioni cognitive (ad esempio gli interventi di potenziamento cognitivo). In letteratura questi diversi tipi di interventi sono stati proposti e studiati per il singolo, mentre pochi ad oggi sono gli interventi appositamente pensati per i gruppi. Nel presente contributo vengono descritte modalità per l'intervento in piccolo gruppo, sulla base dell'analisi della letteratura disponibile e in considerazione dell'esperienza svolta nei campus estivi organizzati
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