1,721,058 research outputs found
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Scanning and transmission electron microscopic study on mesenchymal stem cells derived from equine adipose tissue
No full textIMMUNOLOCALIZATION OF LEPTIN AND ITS RECEPTOR IN THE SWEAT GLAND AND SEBACEOUS GLAND OF THE HORSE.
No full textLeptin (Ob) is a hormone primarily secreted by adipocytes of subcutaneous and visceral fat which binds to a
specific receptor (Ob-R). It is considered an important factor involved in the skin biology. Ob plays a critical
role in the regulation of wound healing processes (1) and it seems to be involved in the control of hair
follicle cycle (2) as well as in the biology of sebaceous gland (3).
The study of Ob in the skin of domestic animals can be of great importance to improve our knowledge in
the biology of the integumentary system. We recently identified the Ob-R in the skin of some domestic
animals such as dog, sheep and cow. The aim of this work is to continue this investigation by evaluating the
presence of Ob and its receptor in the skin of the horse.
Normal skin samples, collected from five animals, were fixed in 10% neutral-buffered formalin. Skin
sections were microwaved in 10 mM citric acid (pH 6.0) for antigen retrieval. The endogenous peroxidase
activity was blocked with 3% peroxidase-blocking solution and non-specific binding was blocked with
normal goat serum. The sections were incubated with mouse monoclonal anti Ob (Fitzgerald) or goat
polyclonal anti Ob-R (Santa Cruz Biotechnology) antibody for 24 hours and, successively, they were
incubated with the goat anti-mouse or goat anti-rabbit biotin conjugate antibody, respectively. The
reaction was detected with the Vectastain ABC kit and visualized with diaminobenzidine.
By observing immunohistochemical treated sections, we evidenced an intense positivity to both Ob and its
receptor in the cells of the apocrine sweat glands in all subjects considered. Moreover, leptin receptor
positivity was observed in the basal cells of sebaceous glands. The staining for Ob was localized in the
cytoplasm of the cells while the staining for Ob-R was both in the cell cytoplasm and membrane. No
staining was observed in other structures of skin.
The result obtained suggests a role of Ob in the regulation of the sweat and sebaceous gland activity.
Apocrine sweat gland cells are both source and target of leptin then, an autocrine or paracrine control by
the hormone can be supposed. Since the cells of sweat glands express only the leptin receptor, it is likely an
endocrine control by the leptin on sweat glands according to Chen et al., (2002)
Identificazione e localizzazione immunoistochimica della leptina e del suo recettore nell'apparato digerente del cervo
No full textImmunohistochemical identificatio and localization of leptin and its receptor in the digestive tract of dee
LEPTIN AND ITS RECEPTOR IN WILD AND DOMESTIC SWINE CARPAL GLANDS
No full texttransmission of scent information and are located in the subcutaneous layer of the carpus caudomedial
surface.
Their histological and histochemical features have been extensively investigated in both domestic and wild
pigs and have provided important information regarding secretion chemical characteristics. The
aim of the present work is to highlight the presence of leptin and its receptor in these glands and to verify
the possibility of difference expressions between wild and domestic subjects, with the pourpose of
providing data useful to better understand the mechanisms regulating their functionality.
Eight clinically healthy adult male pigs, four domestic and four wild, were used. Carpal gland specimens
were obtained immediately after slaughter, fixed in 4% formaldehyde solution and subsequently processed
for embedding in paraffin, following routine tissue preparation procedures.
The immunohistochemical reaction was visualized, utilising the avidin!biotin!complex and the DAB as the
chromogen. The following primary antibodies were used: anti!Ob rabbit polyclonal antibody and anti!Ob!R
goat polyclonal antibody.
The immunohistochemical study showed a strong positivity for leptin and its receptor in the carpal glands
of the animals examined. In particular, the immuno!reaction seems to affect only the cytoplasm of the dark
cells while the clear ones are always negative. Immuno!positivity for leptin and its receptor was not
observed in the epithelium of the ducts or in the connective tissue. In addition, there were no appreciable
differences between domestic and wild swine. Recent studies conducted on some exocrine glands have allowed researchers to highlight how the same are
able to secrete leptin and may represent a target of the action of this protein. This has enabled
them to understand how the action of the Ob protein is much more extensive than first imagined. In this
sense the results obtained in the present study confirm what has previously been stated and allow us to
hypothesize that this gland is able to produce leptin and is itself a target of this molecule that may act by in
autocrine/paracrine manner without being influenced by the different conditions of life
Apoptotic cell death in canine hair follicle
Full text linkApoptotic cell death is an essential homeostatic mechanism involved in the control of cellular turnover in a variety of adult tissues. Cytoplasmic and nuclear condensation morphologically define this process whose biochemical hallmark is extensive DNA fragmentation into discrete oligonucleosomic units. Hair follicle growth and regression has been shown to be correlated with apoptosis in humans, mice, rats and guinea pigs. The present study was carried out to evaluate its implication in canine hair biology in order to define the spatio-temporal relationship between apoptosis and the hair cycle in dogs. As assessed by terminal deoxy-nucleotidyl transferase-mediated d-UTP nick-end-labelling (TUNEL) and by basic histological and ultrastructural assays, apoptotic cells appeared both in the growing and in the regressing follicle epithelium showing the well characterized morphological features described in the previous relevant literature
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