399 research outputs found
Assembly of the CD8alpha/p56(lck) protein complex in stably expressing rat epithelial cells.
No full textAssembly of the CD8alpha/p56(lck) protein complex in stably expressing rat epithelial cells.
No full textAbstractWe have previously characterized the biogenesis of the human CD8α protein expressed in rat epithelial cells. We now describe the biosynthesis, post-translational maturation and hetero-oligomeric assembly of the human CD8α/p56lck protein complex in stable transfectants obtained from the same cell line. There were no differences in the myristilation of p56lck, or in the dimerization, O-glycosylation and transport to the plasma membrane of CD8α, between cells expressing either one or both proteins. In the doubly expressing cells, dimeric forms of CD8α established hetero-oligomeric complexes with p56lck, as revealed by co-immunoprecipitation assays performed with anti-CD8α antibody. Moreover, p56lck bound in these hetero-oligomeric complexes was endowed with auto- and hetero-phosphorylating activity. The present study shows that: (1) the newly synthesized p56lck binds rapidly to CD8α and most of the p56lck is bound to CD8α at steady state; (2) CD8α/p56lck protein complexes are formed at internal membranes as well as at the plasma membrane; and (3) about 50% of complexed p56lck reaches the cell surface
Biosynthesis, Membrane Translocation, and Surface Expression of Sindbis Virus E1 Glycoprotein
No full text“Metal regulation of metallothionein gene expression in mammals” in: Copper transport and its disorder: molecular and cellular aspects (A.Leone and J.F. Mercer Eds.)
No full textPost-translational processing of an O-glycosylated protein, the human CD8 glycoprotein, during the intracellular transport to the plasma membrane
No full textPost-translational processing of an O-glycosylated protein, the human CD8 glycoprotein, during the intracellular transport to the plasma membrane
No full textPost-translational processing of an O-glycosylated protein, the human CD8 glycoprotein, during the intracellular transport to the plasma membrane
No full textReturning culture to peacebuilding : contesting the liberal peace in Sierra Leone
No full textThis thesis investigates the advantages and limitations of applying culture to the analysis of violent conflict and peacebuilding, with a particular focus on liberal peacebuilding in Sierra Leone. While fully aware of the critique of the concept of culture in terms of its uses for the production of difference and ‘otherness,’ it also seeks to respond to the critique of liberal peacebuilding on the account of its low sensitivity towards local culture, which allegedly undermines the peace effort. After a careful examination of the terms of discussion about culture enabled by theoretical approaches to conflict in Chapter 2, the thesis presents a theoretical framework for the analysis of cultural aspects of conflict and peace based on the processes and effects of meaning-generation (Chapter 3), developing the conceptual apparatus and vocabulary for the subsequent empirical study. Instead of bracketing out the recursive nature of cultural theorising, the developed approach embraces the recursive dynamics which arise as a result of cultural ‘embeddedness’ of the analyst and the processes which s/he seeks to elucidate, mirroring similar dynamics in the cultural production of meaning and knowledge. The framework of ‘embedded cultural enquiry’ is then used to analyse the practices of liberal peacebuilding as a particular culture, which shapes the interaction of the liberal peace with its ‘subjects’ and critics as well as framing its reception of the cultural problematic generally (Chapter 4). The application of the analytical framework to the case study investigates the interaction between the liberal peace and ‘local culture,’ offering an alternative reading of the conflict and peace process in Sierra Leone (Chapter 5). The study concludes that a greater attention to cultural meaning-making offers a largely untapped potential for peacebuilding, although any decisions with regard to its deployment will inevitably be made from within an inherently biased cultural perspective
Biosynthesis and oligosaccharide structure of human CD8 glycoprotein expressed in a rat epithelial cell line.
No full textBiosynthesis and oligosaccharide structure of human CD8 glycoprotein expressed in a rat epithelial cell line
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