1,721,048 research outputs found
A novel recombinant of Potato virus Y provides new indications on the genome portions involved in venial necrosis induction in tobacco
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“Aggiornamento sulle avversità delle piante ornamentali, aromatiche ed orticole”
No full textTre esperti fitopatologi, Prof.ssa Maria Grazia Bellardi e Dr Lisa Cavicchi (Università di Bologna), Dr Giuseppe Parrella (CNR, Portici, Napoli), sono stati a disposizione per affrontare assieme i più importanti problemi fitopatologici dovuti a virus e fitoplasmi. I casi esposti sono stati quelli che negli ultimi otto anni si sono verificati nelle coltivazioni al chiuso e/o all’aperto della Piana di Albenga. Oltre agli aspetti sintomatologici e diagnostici, si è affrontato il tema della Difesa e della Prevenzione. Alle tre brevi esposizioni è seguito un dibattito nel corso del quale i Partecipanti si sono rivoltii direttamente ai Relatori per ulteriori chiarimenti
Efects of Alfalfa Mosaic Virus on the chemical composition of sweet basil (Ocimum basilicum L.) essential oil
No full textDETECTION OF ALFALFA MOSAIC VIRUS BY IC-RT-PCR IN VIBURNUM OPULUS L.
No full textViburnum opulus L. (“snowball”, Caprifoliaceae) is a deciduous shrubs used as decorative plants for its fragrant white flowers that bloom in spring. In May 2011, snowball plants showing a virus-like disease were observed in two ornamental public gardens in Imola (Bologna, Emilia Romagna region). Symptoms appeared distributed on only part of the foliage, and consisted of chlorotic rings and spots, line-patterns and, in some cases, vein clearing on younger leaves. Since only Alfafa mosaic virus (AMV) and Cucumber mosaic virus (CMV) have been reported to naturally infect this Viburnum species, and considering that snowball could be an important source of virus infection, symptomatic plants were examined by applying Immunocapture Reverse Transcription-Polymerase Chain Reaction (IC-RT-PCR). Tests were performed on leaf extracts using two polyclonal antisera against an AMV isolate from lettuce and CMV isolate from tomato, respectively. Only AMV was found infecting symptomatic V. opulus. The CPAMV1/CPAMV2 specific AMV primer pairs were used in RT-PCR reactions. A DNA fragment of c.a. 750 bp, covering the entire coat protein gene (CP) of AMV, was obtained after IC-RT-PCR and the product was gel purified and cloned in pGEMT Easy for sequencing. Comparison of the CP sequence of AMV-V. opulus with the CP reference sequences of some AMV isolates, revealed the maximum nucleotide identities with subgroup I isolates. AMV has been previously found infecting V. opulus in Italy in 1995, but the leaf symptomatology observed was only vein yellowing
Molecular and serological detection of Parietaria mottle virus in Phytolacca americana, a new host of the virus
No full textParietaria mottle virus (PMoV) is an emerging virus in Mediterranean countries, responsible for severe disease in tomato and pepper crops in the field and protected cultivation. The principal wild reservoir of PMoV is Parietaria officinalis, and only few additional wild plants have been described as natural reservoirs of the virus. During field survey in southern Italy, several plants of Phytolacca americana showing virus-like symptoms were collected. Serological and molecular assays showed that these plants were infected by PMoV. Sequence comparison of the movement protein gene of the PMoV isolate from P. americana showed the greatest similarity to the corresponding sequence from tomato plants growing nearby. These results indicate that P. ameriacana is a new natural host of PMoV, and further investigation is warranted to establish the potential of this host as reservoir of the virus in the field
Vein yellowing of Hibiscous rosa-sinensis caused by Eggplant mottled dwarf virus in Southern Italy
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