1,720,971 research outputs found
Masculinization of XX Drosophila transgenic flies expressing Ceratitis capitata DSXM isoform.
No full textThe Doublesex (DSX) transcription factor regulates somatic sexual differentiation in Drosophila melanogaster. Female and male isoforms (DSXF and DSXM) are produced due to sex-specific RNA splicing. Here we show that in the distantly related dipteran Ceratitis capitata, the DSXM male-specific isoform is conserved and able to induce masculinization of both somatic and germline tissues when ectopically expressed in XX Drosophila transgenic individuals
The transformer gene in Ceratitis capitata provides a genetic basis for selecting and remembering the sexual fate.
No full textThe medfly Ceratitis capitata contains a gene (Cctra) with structural and functional homology to the Drosophila melanogaster sex-determining gene transformer (tra). Similar to tra in Drosophila, Cctra is regulated by alternative splicing such that only females can encode a full-length protein. In contrast to Drosophila, however, where tra is a subordinate target of Sex-lethal (Sxl), Cctra seems to initiate an autoregulatory mechanism in XX embryos that provides continuous tra female-specific function and act as a cellular memory maintaining the female pathway. Indeed, a transient interference with Cctra expression in XX embryos by RNAi treatment can cause complete sexual transformation of both germline and soma in adult flies, resulting in a fertile male XX phenotype. The male pathway seems to result when Cctra autoregulation is prevented and instead splice variants with truncated open reading frames are produced. We propose that this repression is achieved by the Y-linked male-determining factor (M)
XX and XY male-only medflies obtained by RNAi against the master sex determining Ceratitis transformer gene. Capri, .
No full textTowards the analysis of transcriptome from sexed Ceratitis capitata embryos: how to produce XX male embryos by transgene-mediated RNAi and identify male biased/male determining genes.
No full textIn the agricultural pest Ceratitis capitata, the Mediterranean fruitfly (Medfly), the sex determination is controlled by a Y-linked male determining factor (M-factor) which influences either directly or indirectly the state of activity of the female determining master gene Cctraep (Cctra epigenetic) at 5-7 hours of oviposition. We have developed a Ceratitis transgenic sexing strain which produces male-only progeny (95% efficiency) by transgene-mediated RNAi against the female determiner Cctraep gene. C. capitata XX males are fully fertile and could be successfully mated with XX females thus leading to the production of female-only (XX) progeny. We have prepared polyA+ RNA from XX embryos and from mixed XY-XX embryos, both collected at 5-7 hours from oviposition. We have used a molecular subtractive approach to identify differentially expressed genes in XY versus XX at embryonic stages. We will outline the molecular strategy employed to approach this scientific problem and preliminary data describing the identification of 8 male-biased cDNA positive clones presently under analysis. We started in parallel a transcriptome analysis of the two samples to approach the identification of male-biased genes/male-determining genes by an in silico subtractive analysis
Toward a mRNAseq analysis to explore the transcriptome landscapes of Ceratitis capitata sexed early embryos.
No full textSex determination study of Medfly embryos using bioinformatic
Evolutionarily conserved Drosophila gene regulation by sex-specific splicing to control sex determination of Ceratitis capitata.
No full textThe transformer gene in Ceratitis capitata provides cell memory of Sex Determination.
No full textThe medfly Ceratitis capitata contains a gene (Cctra) with
structural and functional homology to the Drosophila
melanogaster sex-determining gene transformer (tra).
Similar to tra in Drosophila, Cctra is regulated by
alternative splicing such that only females can encode a
full-length protein. In contrast to Drosophila, however,
where tra is a subordinate target of Sex-lethal (Sxl), Cctra
seems to initiate an autoregulatory mechanism in XX
embryos that provides continuous tra female-specific
function and act as a cellular memory maintaining the
female pathway. Indeed, a transient interference with Cctra expression in XX embryos by RNAi treatment can cause
complete sexual transformation of both germline and soma
in adult flies, resulting in a fertile male XX phenotype. The
male pathway seems to result when Cctra autoregulation is
prevented and instead splice variants with truncated open
reading frames are produced. We propose that this
repression is achieved by the Y-linked male-determining
factor (M)
The transformer gene in Ceratitis capitata provides a genetic basis for selecting and remembering the sexual fate.
No full textThe medfly Ceratitis capitata contains a gene (Cctra) with structural and functional homology to the Drosophila melanogaster sex-determining gene transformer (tra). Similar to tra in Drosophila, Cctra is regulated by alternative splicing such that only females can encode a full-length protein. In contrast to Drosophila, however, where tra is a subordinate target of Sex-lethal (Sxl), Cctra seems to initiate an autoregulatory mechanism in XX embryos that provides continuous tra female-specific function and act as a cellular memory maintaining the female pathway. Indeed, a transient interference with Cctra expression in XX embryos by RNAi treatment can cause complete sexual transformation of both germline and soma in adult flies, resulting in a fertile male XX phenotype. The male pathway seems to result when Cctra autoregulation is prevented and instead splice variants with truncated open reading frames are produced. We propose that this repression is achieved by the Y-linked male-determining factor (M)
Transformer is the key switch gene in the sex determination of the mediterranean fruitfly Ceratitis capitata.
No full text- …
