1,721,004 research outputs found
Identification and characterization of the iridoid synthase involved in oleuropein biosynthesis in olive (Olea europaea) fruits
No full textThe secoiridoids are the main class of specialized metabolites present in olive (Olea europaea L.) fruit. In particular, the secoiridoid oleuropein strongly influences olive oil quality because of its bitterness, which is a desirable trait. In addition, oleuropein possesses a wide range of pharmacological properties, including antioxidant, anti-inflammatory, and anti-cancer activities. In accordance, obtaining high oleuropein varieties is a main goal of molecular breeding programs. Here we use a transcriptomic approach to identify candidate genes belonging to the secoiridoid pathway in olive. From these candidates, we have functionally characterized the olive homologue of iridoid synthase (OeISY), an unusual terpene cyclase that couples an NAD (P)H-dependent 1,4-reduction step with a subsequent cyclization, and we provide evidence that OeISY likely generates the monoterpene scaffold of oleuropein in olive fruits. OeISY, the first pathway gene characterized for this type of secoiridoid, is a potential target for breeding programs in a high value secoiridoid-accumulating species. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc
Seed-Specific Expression of AINTEGUMENTA in Medicago truncatula Led to the Production of Larger Seeds and Improved Seed Germination
No full textThe increase of seed size is of great interest in Medicago spp., to improve germination, seedling vigour and, consequently, early forage yield as well as for optimizing seeding techniques and post-seeding management. This study evaluated the effects of the ectopic expression of the AINTEGUMENTA (ANT) cDNA from Arabidopsis thaliana, under the control of the seed-specific USP promoter from Vicia faba, on seed size, germination and seedling growth in barrel medic (Medicago truncatula Gaertn.). All the transgenic T2 barrel medic lines expressing ANT produced seeds significantly larger than those of control plants. Microscopic analysis on transgenic T3 mature seeds revealed that cotyledon storage parenchyma cells were significantly larger and contained larger storage vacuoles than those of the untransformed control. Moreover, the percentage of germination was significantly higher and germination was more rapid in transgenic than in control seeds. Our results indicate that the seed-specific expression of ANT in barrel medic led to larger seeds and improved seed germination, and revealed a regulatory role for ANT in controlling seed size development. © 2014, Springer Science+Business Media New York
Ampelographic and genetic characterisation of ancestral grapevine (Vitis vinifera L.) accessions present in the Umbria Region (Central Italy)
No full textDuring an ongoing effort to recover and preserve local germplasm, 14 accessions of indigenous minor grapevine (Vitis vinifera L.) cultivars from the Umbria Region, Central Italy, were chosen because they had been neglected and were threatened with extinction. Their phenotypic and genetic characteristics were evaluated through an ampelographic study of their shoots, mature leaves, bunches, and berries and by genomic analysis using an international set of nine microsatellite (simple sequence repeat; SSR) markers (VVS2, VVMD5, VVMD7, VVMD27, VrZAG62, VrZAG79, VVMD25, VVMD28, and VVMD32). Comparisons of the SSR profiles of all 14 accessions with grapevine accessions in several databases permitted the identification of unique genotypes, as well as possible synonyms. Information on these older, neglected cultivars will help to reduce the genetic erosion of grapevine germplasm, improve conservation and possible recovery, and assist in the future production of new, distinctive wines
Medicago truncatula Functional Genomics - An Invaluable Resource for Studies on Agriculture Sustainability
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The CM2 and CM3 types of alpha-amylase inhibitor are associated whit triticum aestivum seed chromatin.
No full textLife cycle assessment analysis of Alfalfa and corn for biogas production in a farm case study
Full text linkIn the last years the greenhouse effect has been significantly intensified due to human activities, generating large additional amounts of Greenhouse gases (GHG). The fossil fuels are the main causes of that. Consequently, the attention on the composition of the national fuel mix has significantly grown, and the renewables are becoming a more significant component. In this context, biomass is one of the most important sources of renewable energy with a great potential for the production of energy. The study has evaluated, through an LCA (Life Cycle Assessment) study, the attitude of alfalfa (Medicago sativa) as “no food” biomass alternative to maize silage (corn), in the production of biogas from anaerobic digestion. Considering the same functional unit (1 m3 of biogas from anaerobic digestion) and the same time horizon, alfalfa environmental impact was found to be much comparable to that of corn because it has an impact of about 15% higher than corn considering the total score from different categories and an impact of 5% higher of corn considering only greenhouse gases. Therefore, the analysis shows a similar environmental load in the use of alfalfa biomass in energy production compared to maize. Corn in fact, despite a better yield per hectare and yield of biogas, requires a greater amount of energy inputs to produce 1m3 of biogas, while alfalfa, which requires less energy inputs in its life cycle, has a lower performance in terms of yield. The results show the possibility to alternate the two crops for energy production from an environmental perspective
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