1,721,146 research outputs found
Alessandro de Maigret, The Bronze Age Culture of Ḫawlān aṭ-Ṭiyāl and al-Ḥadā (Republic of Yemen). A First General Report. Contributions by Sandor Bökönyi, Bruno Castiello, Lorenzo Costantini, Francesco di Mario, Francesco G. Fedele, Vincenzo Francaviglia, Adolfo Gianni, Bruno Marcolongo, Alberto M. Palmieri, Annalisa Zarattini (Istituto italiano per il Medio ed Estremo Oriente, Centro Studi e Scavi archeologici). IsMEO, Rome, 1990
No full textRobin Christian. Alessandro de Maigret, The Bronze Age Culture of Ḫawlān aṭ-Ṭiyāl and al-Ḥadā (Republic of Yemen). A First General Report. Contributions by Sandor Bökönyi, Bruno Castiello, Lorenzo Costantini, Francesco di Mario, Francesco G. Fedele, Vincenzo Francaviglia, Adolfo Gianni, Bruno Marcolongo, Alberto M. Palmieri, Annalisa Zarattini (Istituto italiano per il Medio ed Estremo Oriente, Centro Studi e Scavi archeologici). IsMEO, Rome, 1990. In: Bulletin critique des annales islamologiques, n°9, 1992. pp. 203-204
Fibroblasts behavior after N-acetylcysteine and amino acids exposure: extracellular matrix gene expression.
No full textReactive oxygen species (ROS) are chemically reactive molecules with impaired electrons that make them unstable and able to react easily with a great variety of molecules. The main targets of ROS are DNA, proteins, and membrane phospholipids. In the skin, ROS are able to affect the production of collagen and elastin, the main components of the extracellular matrix (ECM). This action contributes to the skin's aging. N-Acetylcysteine (NAC) is an acetylated cysteine residue with excellent anti-oxidant activity that boosts glutathione (GSH) levels. This study evaluates the effect of a solution of NAC and amino acids, which is used in aesthetic medicine as an intra-dermal injective treatment, on fibroblast behavior. To this aim, the expression levels of some ECM-related genes (HAS1, HYAL1 ELN, ELANE, MMP2, MMP3, MMP13, COL1A1, COL3A1) were analyzed on cultured dermal fibroblasts using real-time reverse transcription polymerase chain reaction (RT-PCR). All but two collagen genes were up-regulated after 24 hr of treatment
Regenerative Dentistry and Stem Cells: A Multilineage Differentiation as a Safe and Useful Alternative Way of Harvesting and Selection Adipose Derived Mesenchymal Stem Cells
No full textThis review aims to address procedures and indications for the application of the adipose-derived stem cells (ADSCs) for regenerative dentistry. ADSCs have rarely been used in this particular field; conversely, experience from other clinical fields and basic research seems to recommend the suitability of this application
Comparison of Bio-Revitalizing Injective Products: A Study on Skin Fibroblast Cultures
No full textBio-revitalization is a commonly used technique in aesthetic medicine for improving skin quality and appearance by intra-dermal injection of hyaluronic acid (HA)-containing compounds. The present study compares different HA-containing injectables regarding their effects on cultured skin fibroblasts over time (24, 48, and 72 hr) by using RT-PCR and a panel of genes involved in dermal integrity. Human dermal fibroblasts were seeded on a layer of five different commercial medical devices containing 6.2 mg/mL 10 mg/mL 10%, 13 mg/mL and 20 mg/mL, respectively, of HA. The products differ not only in HA concentration but also in the content and quality of other ingredients; moreover, one of these products contained cross-linked HA. Differences among medical devices were found. In particular, HA concentration seems to be inversely correlated to elastin gene activation. Regarding the neutrophil elastase gene, the two medical devices with the higher concentration of HA displayed the greater effect. Genes encoding for hyaluronan synthase 1, hyaluronidase 1, and desmoplakin were enhanced, but the HA content of the different products did not seem to be directly related to gene activation. Therefore, the explanation for the differences must be studied further with respect to elements that are distinctive for each device. For the physician, it is important to choose which drugs or medical devices can be used and in what protocols. The present study performed a comparison that can be useful in better addressing the skin improvement therapies for aging and in its prevention
Non-syndromic Cleft Palate: An Overview on Human Genetic and Environmental Risk Factors
Full text linkThe epithelial and mesenchymal cells involved in early embryonic facial development are
guided by complex regulatory mechanisms. Any factor perturbing the growth, approach
and fusion of the frontonasal and maxillary processes could result in orofacial clefts
that represent the most common craniofacial malformations in humans. The rarest and,
probably for this reason, the least studied form of cleft involves only the secondary
palate, which is posterior to the incisive foramen. The etiology of cleft palate only is
multifactorial and involves both genetic and environmental risk factors. The intention
of this review is to give the reader an overview of the efforts made by researchers to
shed light on the underlying causes of this birth defect. Most of the scientific papers
suggesting potential environmental and genetic causes of non-syndromic cleft palate are
summarized in this review, including genome-wide association and gene–environment
interaction studies
Human genetic factors in nonsyndromic cleft lip and palate: An update
No full textNonsyndromic cleft lip and/or palate (or orofacial cleft, OFC) is a malformation characterized by an incomplete separation between nasal and oral cavities without any associated anomalies. The last point defines the distinction between syndromic and nonsyndromic OFC. Nonsyndromic OFC is one of the most common malformations among live births and is composed of two separate entities: cleft lip with or without cleft palate (CL+/-P) and cleft palate isolated (CPI). Because of the complex etiology of nonsyndromic OFC, which is due to the differences between CL+/-P and CPI, and the heterogeneity of each group, caused by the number of genes involved, the type of inheritance, and the interaction with environmental factors, we reviewed those genes and available loci in the literature whose involvement in the onset of nonsyndromic OFC has more sound scientific evidence. Genetic studies on human populations have demonstrated that CL+/-P and CPI have distinct genetic backgrounds and, therefore, environmental factors probably disclose only these malformations. In CL+/-P several loci, OFC from 1 to 10 have been identified. The first locus, OFC1, has been mapped to chromosome 6p24. Other CL+/-P loci have been mapped to 2p13 (OFC2), 19q13.2 (OFC3) and 4q (OFC4). OFC5-8 are identified by mutations in the MSX1, IRF6, PVRL1, and TP73L gene, respectively. OFC9 maps to 13q33.1-q34, whereas OFC10 is associated with haploinsufficiency of the SUMO1 gene. In addition, MTHFR, TGF-beta3, and RARalpha play a role in cleft onset. In CPI one gene has been identified (TBX22) at present, but others are probably involved. Greater efforts are necessary in order to have a complete picture of the main factors involved in lip and palate formation. These elements will permit us to better understand and better treat patients affected by OFC
A comparison between genetic portraits of normal osteoblasts and osteosarcoma cell lines
No full textOsteosarcoma (OS) is the most frequent malignant bone tumor occurring in young patients in the first two decades of life. Metastases are the cause of 90\% of cancer deaths for patients with OS. OS of the jaw is rare and aggressive malignancy constitutes approximately 5-13\% of all cases of skeletal OS. Chemotherapy plus surgery are the first choice for treatment.Because OS cell lines (OCLs) should share a common pathway with primary OS and new drugs are screened in in vitro systems, new insight about the genetic profiling of OCLs is of paramount importance to a better understanding of the molecular mechanism of this rare tumor and detecting a potential target for specific therapy.The SAOS2 and TE85 cell lines were analysed using DNA microarrays containing 19,000 genes. Several genes in which expression was significantly differentially expressed in OCLs vs. normal osteoblast (NO) were detected.The differentially expressed genes cover a broad range of functional activities: (a) cell cycle regulation, (b) cell differentiation, (c) apoptosis, and (d) immunity.The reported data can be relevant to a better understanding of the biology of OS and as a model for comparing the effect of drugs used in OS treatment
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Aquolab ® ozone-therapy is an efficient adjuvant in the treatment of chronic periodontitis: A case-control study
No full textAim: The objective of this study was to compare the efficacy of supportive periodontal therapy (i.e., scaling and root planning [SRP]) alone versus Aquolab ® ozone therapy used in association with SRP in the treatment of chronic periodontitis in adult patients. Materials and Methods: A total of 20 patients with a diagnosis of chronic periodontitis (40 localized chronic periodontitis sites) were enrolled. None of these patients have previously received any surgical or nonsurgical periodontal therapy and demonstrated radiographic evidence of moderate bone loss. Two nonadjacent sites in separate quadrants were selected in each patient to verify treatment efficacy (split-mouth design). Microbial analysis was analyzed at baseline and the 7 th day after treatment. SPSS program and paired simple statistic t-test were used to detect statistically significant differences. Results: There was a statistically significant reduction of Tannerella forsythia loading in sites treated with ozone therapy respect to those treated with SPR alone. A similar trend was obtained also for additional 5 species and for total bacterial loading (CBT). These results were obtained with a single local application of ozone therapy just after SPR and with a molecular control 7 th day after treatment. Conclusion : Aquolab ® ozone therapy in is effective in reducing the CBT in pockets of patients affected by periodontitis. It is an efficacy medical device to be used as adjuvant therapy to be added to SRP in the management of moderate to severe chronic periodontitis
- …
