1,721,031 research outputs found
A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function
Full text linkAntonin W, Holroyd C, Fasshauer D, Pabst S, Fischer von Mollard G, Jahn R. A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function. EMBO JOURNAL. 2000;19(23):6453-6464.Sets of SNARE proteins mediate membrane fusion by assembling into core complexes. Multiple SNAREs are thought to function in different intracellular trafficking steps but it is often unclear which of the SNAREs cooperate in individual fusion reactions. We report that syntaxin 7, syntasin 8, vti1b and endobrevin/ VAMP-8 form a complex that functions in the fusion of late endosomes. Antibodies specific for each protein coprecipitate the complex, inhibit homotypic fusion of late endosomes in vitro and retard delivery of endocytosed epidermal growth factor to lysosomes. The purified proteins form core complexes with biochemical and biophysical properties remarkably similar to the neuronal core complex, although each of the four proteins carries a transmembrane domain and three have independently folded N-terminal domains. Substitution experiments, sequence and structural comparisons revealed that each protein occupies a unique position in the complex, with syntaxin 7 corresponding to syntasin 1, and vti1b and syntaxin 8 corresponding to the N- and C-terminal domains of SNAP-25, respectively. We conclude that the structure of core complexes and their molecular mechanism in membrane fusion is highly conserved between distant SNAREs
Interpreting the ash profile along ombrotrophic bog profiles: atmospheric dust depositions vs. mineralization processes
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Comparative evaluation of the mineralogical composition of Sphagnum peat and their corresponding humic acids, and implications for understanding past dust depositions
No full textWith 6.5 m of peat accumulated during the past ca. 15,000 years, Etang de la Gruère (EGr) represents the longest continuous record of atmospheric dust deposition in northern hemisphere. This paper presents a comparative study of the mineralogical composition of Sphagnum peat samples and their corresponding humic acids (HA) from a peat core collected at EGr bog. The purpose of this study was to better understand the dominant process responsible for the amount and distribution of mineral matter in ombrotrophic (i.e., rain-fed) peat. Specifically, the goal was to separate the relative importance of the changing rates of atmospheric mineral dust deposition (during the past two millennia) from the mineralization of organic matter (OM), for the distribution of the ash fraction of the peat profile.
The results suggest that variations in ash content at EGr are mainly the result of an increase in the rate of supply of dust particles and cannot be attributed simply, or exclusively, to differences in the degree of OM decay: evidence is provided by both acid insoluble ash (AIA) profiles and the correlations among lithogenic elements (Al, Si, Ti, Y, and Zr), total ash content and AIA. Moreover, our findings suggest that part of the AIA occurring in peat extremely is stable, and is unaffected by the extreme chemical conditions used to extract the HA fraction: this may be partly due to the inherent stability of quartz and “heavy minerals” such as zircon and rutile, but organic coatings developed on mineral surfaces during peat diagenesis, or the formation of some other kind of organo-mineral complex, might also play a role. Consequently, testimonies of past dust depositions can be observed also into the recalcitrant HA fraction of the peat
Interpreting the ash profile along ombrotrophic bog profiles: atmospheric dust depositions vs. mineralization processes
No full textMagma chamber evolution prior to the Campanian Ignimbrite and Neapolitan Yellow Tuff eruptions (Campi Flegrei, Italy).
No full textGoing Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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