1,720,994 research outputs found
Aspetti citologici, citofluorimetrici e molecolari dell'infiltrato linfoide intratiroideo in citologia aspirativa per ago sottile.
No full text.INTRODUZIONE: L'infiltrato linfoide intratiroideo (TLI) nella tiroidite di Hashimoto (HT) rappresenta il substrato su cui può insorgere un linfoma tiroideo (TL). La citologia aspirativa per ago sottile (FNC) è uno strumento elettivo per diagnosticare e studiare la HT poiché non vi sono, per questa patologia, non ha generalmente indicazioni chirurgiche per fini diagnostici o terapeutici. Scopo del nostro studio è stato classificare il TLI nella HT comparando gli aspetti citologici ed i dati citofluorimetrici (FC) e valutando il rapporto κ/λ delle catene leggere ed il loro assetto molecolare.MATERIALI E METODI: FNC è sono stati eseguiti su 34 pazienti con HT che lamentavano ingrandimento palpabile, diffuso o nodulare, della tiroide. Due o tre prelievi sono stati eseguiti ed utilizzati per preparare strisci tradizionali, FC e sospensioni cellulari in RNAlaterTM per determinazioni molecolari. FC è stata eseguita utilizzando i seguenti anticorpi fluoresceinati: CD3, CD5, CD10, CD19, catene leggere κ e λ. In quattro casi, dalle cellule aspirate e sospese in RNAlaterTM, DNA ad alto peso molecolare è stato estratto e processato mediante Polymerase Chain Reaction (PCR) per amplificare le sequenze genomiche codificanti per la regione variabile (VDJ) delle catene pesanti. Un analisi statistica è stata infine eseguita per valutare possibili associazioni tra presentazione clinico-ecografica, pattern citologico e fenotipo del TLI. L'espressione delle catene leggere è stata valutata come percentuale delle cellule esprimenti le singole catene (≤20%, >20%) e come rapporto κ/λ.RISULTATI: I casi studiati sono stati classificati citologicamente come “lymphocytic”, “lymph-node like” e “mixed”. FC ha evidenziato linfociti T (CD3+,CD5+) in tutti i casi e linfociti B (CD19+,CD10+/-) in 22 casi. Le catene leggere sono risultate espresse in 30 casi; in 13 casi in meno del 20% delle cellule ed in 17 casi in più del 20%. Cinque casi mistrarono piccoli sbilanciamenti del rapporto κ/λ e la PCR ha mostrato diffuse bande nel gel e curve gaussiane all' heteroduplex. L'analisi statistica mostrò associazioni significative tra pattern “lymphocytic” e fenotipo T e tra “lymph-node like” pattern e fenotipo B. Una associazione statisticamente significativa è stata osservata tra restrizione per le catene leggere e bassa espressione delle catene stesse (P<0.005).CONCLUSIONI: Il pattern citologico del TLI nella HT è rappresentativo della presentazione clinica e del fenotipo cellulare prevalente. Piccoli sbilanciamenti delle catene leggere non sono supportati da riarrangiamenti genetici delle catene pesanti. FNA associato alla tipizzazione citofluorimetrica può contribuire allo studio e alla diagnosi differenziale tra TLI florida e TL
Histological and fine needle aspiration cytological features of Hashimoto thyroditis-associated ?angiomatoid? papillary thyroid carcinoma
No full textOccult lymph node metastasis from desmoplastic small round cell tumor diagnosed by fine needle aspiration cytology. A case report.
No full textDesmoplastic small round cell tumor (DSRCT) is a rare but well-defined neoplasm generally forming in the abdominal or pelvic cavity of young males and has distinct clinical, immunohistochemical and molecular features. Cytologic features of DSRCT have been described on fine needle aspiration of primary tumors. An occult lymph node metastasis of DSRCT diagnosed through the cytologic features, a basic immunocytochemical panel and DNA ploidy evaluation on cytospins obtained by fine needle aspiration is reported. CASE: Aspiration cytology was performed on an inguinal lymph node from a 20-year-old male. A Diff-Quik-stained smear showed mature lymphocytes and groups of undifferentiated, small cells with scanty cytoplasm, dense and coarse chromatin, and small nucleoli. Basic immunocytochemical stains showed negativity for leukocyte-common antigen and neuron-specific enolase and positivity for cytokeratin cocktail (Cam 5.2), vimentin and desmin, the last with characteristic paranuclear dotlike positivity. DNA ploidy evaluation showed an aneuploid histogram with a low 5c exceeding rate. CONCLUSION: Cytologic and immunocytochemical features suggest the diagnosis of DSRCT on fine needle aspiration cytology samples even in cases of a metastatic, unknown primary tumor. Because of the tumor's aggressiveness, a rapid and accurate diagnosis is required
Contabilità dei singoli agenti inquinanti: descrizione di un approccio metodologico per la Salvaguardia Ambientale
No full textMetodologia innovativa per controlli ambiental
Fine needle aspiration biopsy of hepatic focal fatty change. A report of two cases
No full textIn rare instances, hepatic steatosis produces a circumscribed, nodular lesion described as focal fatty liver change (FFLC). The ultrasonographic and computed tomographic patterns are those of an isointense or hyperechoic nodule, sometimes simultating metastasis. CASES: Fine needle aspiration biopsy was performed under ultrasonographic control in two men aged 65 and 67 years who had previously undergone emicolectomy and gastrectomy for adenocarcinoma. Routine hepatic ultrasound showed solitary nodules, of 3 and 4 cm in diameter. The microscopic patterns were similar and highly cellular in both cases. Cells were isolated or organized in sheets and characterized by large, intracytoplasmic, clear vacuoles that displaced nuclei to the periphery of the cells, flattening them against the cytoplasmic membrane and giving these cells a signet-ring appearance. Nuclei were generally round and nucleolated or dense and hyperchromatic when flattened onto the cytoplasmic membrane. Normal hepatocytes were interspersed in the background, and in some areas of the slides hepatocytes with one or more small intracytoplasmic vacuoles with cytologic features intermediate between those of vacuolated cells and normal hepatocytes were present. Digested periodic acid-Schiff staining, performed on destained, fixed smears, gave negative results. The cytologic diagnosis was FFLC. Clinical and echographic follow-up confirmed the cytologic diagnosis. CONCLUSION: The ultrasonographic and microscopic features of FFLC may mimic those of metastasis. A proper cytologic diagnosis may contribute to the diagnostic workup of these rare lesions
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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