1,721,187 research outputs found
Forgetting RSPO1
No full textThis clinical condition, which is extremely rare, is very interesting
for researchers involved in studies regarding sex determination
processes, because the molecular analysis of such patients could
lead to the discovery of genes involved in testis differentiation in
the absence of the master gene SRY. Unfortunately, the article
includes a number of inaccuracies and omissions regarding the state
of the art on clinical features and genetic studies of this condition in
human patients and animal models. Consequently some aspects of it
ought to be revised or clarified
Molecular Mechanisms of Sexual Development
No full textGonadal cellular organization is very similar in all vertebrates, though different processes can trigger bipotential gonads to develop into either testes or ovaries. While mammals and birds, apart from some exceptions, show genetic sex determination (GSD), other animals, like turtles and crocodiles, express temperature-dependent sex determination. In some groups of animals, GSD can also be overridden by hormone or temperature influences, indicating how fragile this system can be. This review aims to explain the fundamental molecular mechanisms involved in mammalian GSD, mainly referring to mouse as a major model. Conceivably, other mammals might show a molecular mechanism different from the commonly investigated murine species
F.I.S.H. mapping in Cattle (Bos taurus) is not yet out of fashion
No full textPhysical mapping of genes by fluorescence in situ hybridization (FISH) seems to be out of fashion in
species whose assembled genome sequences are available. However, in this work we evidence the existence of
errors in gene location in the Btau_4.0 assembly. We show that DFNA5 and CHCHD6 genes are located on
BTA4 and BTA22, respectively, instead of BTA10 and BTA3, as displayed by Btau_4.0. This report emphasizes
the need to verify the data on physical localization of genes in the cattle genome (at least by taking into account
comparative data reported in available papers) and the need to improve the cattle genome assembly. Our results
indicate that FISH mapping in cattle is still useful
Stability of tumor markers CA 19.9, CA 125, and CA 15.3 in serum obtained from plain tubes and tubes containing thixotropic gel separator
No full text
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Molecular analysis of a XY mare with gonadal dysgenesis
No full textIn this study, cytogenetic analysis of an infertile mare revealed a 64, XY karyotype. The XY sex-reversed animal had a female phenotype with gonadal dysgenesis. Using Southern blot analysis, we tested for the presence of two Y-specific genes SRY and ZFY by using DNA isolated from peripheral blood leukocytes. The results showed that at least the DNA-binding domain of the SRY gene was deleted from the Y chromosome of the XY mare but that the ZFY gene was present on this chromosome
- …
