8,843 research outputs found
Pinguicula (Lentibulariaceae) in central Italy: taxonomic study
No full textA morphometric and taxonomic study of Pinguicula in central Italy was carried out. Six allopatric units occur in this area, all belonging to sect. Pinguicula: P. fiorii Tammaro & Pace, P. vulgaris L. subsp. vulgaris and four new taxa: P. vulgaris subsp. anzalonei Peruzzi & F. Conti subsp. nova, P. vulgaris subsp. ernica Peruzzi & F. Conti subsp. nova, P. vulgaris subsp. vestina F. Conti & Peruzzi subsp. nova and P. vallis-regiae F. Conti & Peruzzi sp. nova. A key to the Italian species and subspecies of Pinguicula is provided
The geological map of Sardinia (Italy) at 1:250,000 scale
No full textOver the last 25 years the Italian national geological mapping program of the Italian Geological Survey (CARG Project, italian: Progetto Carta Geologica) at 1:50,000 scale has led to significant improvements in the geological knowledge for the Island of Sardinia (Italy). As a result, about one half of the island now is covered by new geological maps with 1:10,000–1:25,000 accuracy and geological maps at the 1:50,000 scale whose explanatory notes are available electronically. At the beginning of the CARG Project a geological map for Sardinia Island at 1:200,000 scale was published [Carmignani, L. (1996). Carta Geologica della Sardegna (1:200.000). Servizio Geologico Nazionale, Regione Autonoma della Sardegna], summarizing all the geological information available at that time, and a book with explanatory notes for the map was later published [Carmignani, L., Oggiano, G., Barca, S., Conti, P., Salvadori, I., Eltrudis, ... Pasci, S. (2001). Geologia della Sardegna: Note Illustrative della Carta Geologica della Sardegna in scala 1:200.000, Memorie Descrittive della Carta Geologica d'Italia (Vol. 60). Roma: Servizio Geologico d'Italia, 283 pp]. The enclosed Geological map of Sardinia at 1:250,000 scale incorporates all maps of the CARG Project, unpublished author studies and recently published maps and represents the most updated synthesis of an area characterised by a complex geological evolution that, with few exceptions, can be considered continuous during the last 540 Ma. The main events that influenced the geology of the island are the Variscan orogen that deeply involved the passive margin of North Gondwana and then the complex episodes that occurred in the present-day Mediterranean area after the accretion of Pangea up to the opening of the Tyrrhenian basin
Design, synthesis and biological characterization of PfGAPDH inhibitors
No full textMalaria has a tremendous health, social and economic impact on people living in tropical and subtropical regions of the world. WHO estimated 214 million cases and 438 000 deaths in 2015 and 3.2 billion people are globally at risk of infection1. Available treatments are becoming progressively less effective, mainly because parasites are constantly acquiring resistance toward the drug in use. Therefore, there is an urgent need of developing therapeutic agents acting on new targets.
Plasmodium parasites (P. falciparum is the most lethal), in their amastigote phase, produce energy only through glycolysis. In this pathway, GAPDH catalyses the first energy productive step and thus represent a valuable target for drug discovery. Known GAPDH inhibitors that bind to the active site are small nonselective molecules (iodoacetamide, 3-bromopyruvate), which irreversibly react with cysteine residues of the enzyme, including the catalytic one. We designed and synthesized the first series of inhibitors characterized by a 3-bromoisoxazoline warhead2, inspired by 3-bromoacivicin, a known inhibitor of reactive cysteine containing enzymes3. The compounds were assayed for the inhibitory activity on the P. falciparum isolated enzyme showing a biphasic irreversible inactivation. MS/MS studies of the digested protein demonstrated the mild reactivity of the exploited warhead, which only reacts with catalytic Cys (activated by a His residues). The human ortholog of GAPDH is only partially inhibited (up to 25%). We explained this through a negative cooperativity that prevents the alkylation of all the four monomers of hGAPDH, resulting in selectivity among the two isoforms of GAPDH4. Compounds were also assayed on cultures of the bloodstream form of P. falciparum and human cell lines in order to determine their in vitro antiparasitic activity and toxicity.
The goal of this project is to obtain compounds with improved potency and pharmacokinetic properties, suitable for in vivo studies on animal model of P. falciparum infections.
1. http://www.who.int/mediacentre/factsheets/fs094/en/
2. Bruno, S.; Pinto, A.; Paredi, G.; Tamborini, L.; De Micheli, C.; La Petra, V.; Marinelli, L.; Novellino, E.; Conti, P.; Mozzarelli, A. J. Med. Chem. 2014, 57(17), 7465-7471.
3. a) Conti, P.; Pinto, A.; Wong, P.E.; Major, L.L.; Tamborini, L.; Iannuzzi M.C.; De Micheli, C.; Barrett, M.P.; Smith, T.K. ChemMedChem 2011, 6(2), 329-333. b) Tamborini, L.; Pinto, A.; Smith, T.K.; Major, L.L.; Iannuzzi, M.C.; Cosconati, S.; Marinelli, L.; Novellino, E.; Lo Presti, L.; Wong, P.E.; Barrett, M.P.; De Micheli, C.; Conti, P. ChemMedChem 2012, 7(9), 1623-1634.
4. Bruno, S.; Margiotta, M.; Pinto, A.; Cullia, G.; Conti, P.; De Micheli, C.; Mozzarelli, A. Bioorg. Med. Chem. 2016, 24(12), 2654-2659
The geological map of Sardinia (Italy) at 1:250,000 scale
No full textOver the last 25 years the Italian national geological mapping program of the Italian Geological Survey (CARG Project, italian: Progetto Carta Geologica) at 1:50,000 scale has led to significant improvements in the geological knowledge for the Island of Sardinia (Italy). As a result, about one half of the island now is covered by new geological maps with 1:10,000–1:25,000 accuracy and geological maps at the 1:50,000 scale whose explanatory notes are available electronically. At the beginning of the CARG Project a geological map for Sardinia Island at 1:200,000 scale was published [Carmignani, L. (1996). Carta Geologica della Sardegna (1:200.000). Servizio Geologico Nazionale, Regione Autonoma della Sardegna], summarizing all the geological information available at that time, and a book with explanatory notes for the map was later published [Carmignani, L., Oggiano, G., Barca, S., Conti, P., Salvadori, I., Eltrudis, ... Pasci, S. (2001). Geologia della Sardegna: Note Illustrative della Carta Geologica della Sardegna in scala 1:200.000, Memorie Descrittive della Carta Geologica d'Italia (Vol. 60). Roma: Servizio Geologico d'Italia, 283 pp]. The enclosed Geological map of Sardinia at 1:250,000 scale incorporates all maps of the CARG Project, unpublished author studies and recently published maps and represents the most updated synthesis of an area characterised by a complex geological evolution that, with few exceptions, can be considered continuous during the last 540 Ma. The main events that influenced the geology of the island are the Variscan orogen that deeply involved the passive margin of North Gondwana and then the complex episodes that occurred in the present-day Mediterranean area after the accretion of Pangea up to the opening of the Tyrrhenian basin
The radical right in Europe, between slogans and voting behavior. IHS Political Science Series No. 123, July 2011
No full textThe paper analyzes the radical right‘s attitudes toward the EU focusing in particular on the level of congruence between the programmatic statements of the central office and the voting behavior of their MEPs. It shows that although radical right parties represent a source of opposition to the EU, within the EP they express their dissent making use of the rules of the game, voting with the opposition more than the other forces do, but voting almost as much with the majority. The party public office in the EP is inserted in the legislative process and even more collusive with the other parties of both sides of the political spectrum than the Eurosceptical rhetoric and statements of central office makes the public believe
A highly efficient flow reactor process for the synthesis of N-Boc-3,4-dehydro-L-proline methyl ester
No full textThe multi-step preparation of N-Boc-3,4-dehydro-L-proline methyl ester using a modular flow reactor is
reported. The use of immobilised reagents and scavengers in pre-packed glass tubes allows us to obtain the pure product in 87% overall yield, 97% purity, and >98% enantiomeric excess without any additional purification step. Our flow-based protocol enables the rapid multi-gram synthesis (about 9 g/12 h) of the desired product
OTS1 and OTS2 SUMO proteases link plant development and survival under salt stress
No full textHigh salinity is an important factor limiting agriculture as major crops are salt sensitive. Understanding salt stress signalling is key to producing salt tolerant crops. The small ubiquitin-like modifier (SUMO) is a crucial regulator of signalling proteins in eukaryotes. Attachment of SUMO onto substrates is reversible and SUMO-proteases which specifically cleave the SUMO-substrate linkages play a vital regulatory role during SUMOylation. We have identified two SUMO proteases OTS1 and OTS2 that act redundantly to regulate salt stress responses in Arabidopsis. ots1 ots2 double mutants show extreme sensitivity to salt. However during non-salt conditions, ots1 ots2 double mutants are phenotypically similar to wild-type plants in terms of growth and development. Overexpressing SUMO1 in the ots1 ots2 double mutants severally diminishes plant size as quantified by rosette diameter even under non-stressed conditions. This reduction in plant growth is reminiscent of ots1 ots2 double mutants under salt stress. Our data indicates that overSUMOylation of target proteins can have severe effects on plant growth and that SUMO proteases like OTS1/2 are key to maintaining cellular balance of SUMOylation. We propose that upon environmental stress the hyperSUMOylation of key target proteins act to retard growth to survive stress periods. Addendum to: Conti L, Price G, O'Donnell E, Schwessinger B, Dominy P, Sadanandom A. Small ubiquitin-like modifier proteases OVERLY TOLERANT TO SALT1 and -2 regulate salt stress responses in Arabidopsis
I conti Alberti dalla dimensione regionale alla signoria appenninica
No full textI conti Alberti dalla dimensione regionale alla signoria appenninic
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